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- PDB-6u6x: Human SAMHD1 bound to deoxyribo(C*G*C*C*T)-oligonucleotide -

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Basic information

Entry
Database: PDB / ID: 6u6x
TitleHuman SAMHD1 bound to deoxyribo(C*G*C*C*T)-oligonucleotide
Components
  • DNA SC-GS-SC-SC-DT
  • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
KeywordsHYDROLASE / SAMHD1 / HIV / restriction factor / innate immunity / dNTPase / deoxyribo-oligonucleotide / phosphorothioate
Function / homology
Function and homology information


Nucleotide catabolism / Hydrolases; Acting on ester bonds; Triphosphoric-monoester hydrolases / deoxynucleoside triphosphate hydrolase activity / triphosphoric monoester hydrolase activity / dGTP binding / dATP catabolic process / dGTPase activity / tetraspanin-enriched microdomain / DNA strand resection involved in replication fork processing / deoxyribonucleotide catabolic process ...Nucleotide catabolism / Hydrolases; Acting on ester bonds; Triphosphoric-monoester hydrolases / deoxynucleoside triphosphate hydrolase activity / triphosphoric monoester hydrolase activity / dGTP binding / dATP catabolic process / dGTPase activity / tetraspanin-enriched microdomain / DNA strand resection involved in replication fork processing / deoxyribonucleotide catabolic process / dGTP catabolic process / negative regulation of type I interferon-mediated signaling pathway / regulation of innate immune response / somatic hypermutation of immunoglobulin genes / RNA nuclease activity / double-strand break repair via homologous recombination / Interferon alpha/beta signaling / site of double-strand break / single-stranded DNA binding / protein homotetramerization / defense response to virus / nucleic acid binding / immune response / innate immune response / DNA damage response / GTP binding / RNA binding / zinc ion binding / nucleoplasm / identical protein binding / nucleus / plasma membrane
Similarity search - Function
HD domain profile. / HD domain / HD domain / SAM domain (Sterile alpha motif) / SAM domain profile. / Metal dependent phosphohydrolases with conserved 'HD' motif. / Sterile alpha motif. / HD/PDEase domain / Sterile alpha motif domain / Sterile alpha motif/pointed domain superfamily
Similarity search - Domain/homology
DNA / Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.58 Å
AuthorsTaylor, A.B. / Bhattacharya, A. / Wang, Z. / Ivanov, D.N.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI104476 United States
CitationJournal: Nat Commun / Year: 2021
Title: Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification.
Authors: Yu, C.H. / Bhattacharya, A. / Persaud, M. / Taylor, A.B. / Wang, Z. / Bulnes-Ramos, A. / Xu, J. / Selyutina, A. / Martinez-Lopez, A. / Cano, K. / Demeler, B. / Kim, B. / Hardies, S.C. / Diaz- ...Authors: Yu, C.H. / Bhattacharya, A. / Persaud, M. / Taylor, A.B. / Wang, Z. / Bulnes-Ramos, A. / Xu, J. / Selyutina, A. / Martinez-Lopez, A. / Cano, K. / Demeler, B. / Kim, B. / Hardies, S.C. / Diaz-Griffero, F. / Ivanov, D.N.
History
DepositionAug 30, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Sep 2, 2020Provider: repository / Type: Initial release
Revision 1.1Sep 22, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2Mar 16, 2022Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3Oct 11, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
B: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
C: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
D: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
E: DNA SC-GS-SC-SC-DT
G: DNA SC-GS-SC-SC-DT
F: DNA SC-GS-SC-SC-DT
H: DNA SC-GS-SC-SC-DT
hetero molecules


Theoretical massNumber of molelcules
Total (without water)253,30512
Polymers253,0438
Non-polymers2624
Water21612
1
A: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
D: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
E: DNA SC-GS-SC-SC-DT
H: DNA SC-GS-SC-SC-DT
hetero molecules


Theoretical massNumber of molelcules
Total (without water)126,6526
Polymers126,5224
Non-polymers1312
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
C: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
G: DNA SC-GS-SC-SC-DT
F: DNA SC-GS-SC-SC-DT
hetero molecules


Theoretical massNumber of molelcules
Total (without water)126,6526
Polymers126,5224
Non-polymers1312
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)69.360, 81.870, 106.000
Angle α, β, γ (deg.)69.720, 76.300, 82.770
Int Tables number1
Space group name H-MP1
Space group name HallP1
Symmetry operation#1: x,y,z

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Components

#1: Protein
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 / dNTPase / Dendritic cell-derived IFNG-induced protein / DCIP / Monocyte protein 5 / MOP-5 / SAM ...dNTPase / Dendritic cell-derived IFNG-induced protein / DCIP / Monocyte protein 5 / MOP-5 / SAM domain and HD domain-containing protein 1 / hSAMHD1


Mass: 61740.523 Da / Num. of mol.: 4 / Mutation: D311A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SAMHD1, MOP5 / Plasmid: pET30a / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q9Y3Z3, Hydrolases; Acting on ester bonds; Triphosphoric-monoester hydrolases
#2: DNA chain
DNA SC-GS-SC-SC-DT


Mass: 1520.251 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.17 Å3/Da / Density % sol: 43.19 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop
Details: 0.2 M sodium tartrate dibasic, 20% polyethylene glycol 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97923 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 24, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97923 Å / Relative weight: 1
ReflectionResolution: 2.58→61.66 Å / Num. obs: 65305 / % possible obs: 97.8 % / Redundancy: 2.9 % / Biso Wilson estimate: 67.28 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.05 / Rpim(I) all: 0.035 / Net I/σ(I): 12.3
Reflection shellResolution: 2.58→2.65 Å / Redundancy: 3 % / Rmerge(I) obs: 0.971 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 4856 / CC1/2: 0.619 / Rpim(I) all: 0.684 / % possible all: 97.9

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3U1N

3u1n


Resolution: 2.58→61.66 Å / SU ML: 0.3274 / Cross valid method: THROUGHOUT / σ(F): 1.95 / Phase error: 28.9907
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2365 2261 3.46 %
Rwork0.1955 63024 -
obs0.197 65285 97.76 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 99.29 Å2
Refinement stepCycle: LAST / Resolution: 2.58→61.66 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13222 0 277 12 13511
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.016513838
X-RAY DIFFRACTIONf_angle_d1.193418670
X-RAY DIFFRACTIONf_chiral_restr0.06981986
X-RAY DIFFRACTIONf_plane_restr0.00772363
X-RAY DIFFRACTIONf_dihedral_angle_d20.48325115
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.58-2.640.38611410.30883920X-RAY DIFFRACTION97.76
2.64-2.70.32471400.29113917X-RAY DIFFRACTION97.64
2.7-2.760.33371410.26893944X-RAY DIFFRACTION97.91
2.76-2.840.29051420.25643937X-RAY DIFFRACTION97.82
2.84-2.920.30851430.25393972X-RAY DIFFRACTION97.95
2.92-3.020.34181390.24683943X-RAY DIFFRACTION97.91
3.02-3.130.32411400.25263968X-RAY DIFFRACTION98.25
3.13-3.250.32831420.23093933X-RAY DIFFRACTION97.82
3.25-3.40.26731430.21953957X-RAY DIFFRACTION98.06
3.4-3.580.20831430.20073956X-RAY DIFFRACTION98.02
3.58-3.80.1981420.18473945X-RAY DIFFRACTION98.08
3.8-4.10.21631430.17423940X-RAY DIFFRACTION97.82
4.1-4.510.19971430.16113953X-RAY DIFFRACTION97.9
4.51-5.160.19451400.15593939X-RAY DIFFRACTION97.94
5.16-6.50.24091420.19623960X-RAY DIFFRACTION98.13
6.5-61.660.21821370.18213840X-RAY DIFFRACTION95.19
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.51396287049-0.238695690287-0.2861127046382.699229705290.05184090964061.445395936830.03355226087370.132805151204-0.3028922782270.0913060911844-0.0551750822615-0.6570443782390.08268081316890.385190115770.01603743991370.468977171792-0.000404126650698-0.004739022582380.534119663097-0.001609367551460.54788035099256.4328065969-17.164924458136.5813442747
26.95383094184-1.64975389131-2.308525843131.341449371470.3956415379190.8176471397650.104650875223-0.322942511738-1.0982065151-0.153591760734-0.05645592193120.2914940577270.3514210478950.195410278143-0.01569253818440.655975268565-0.0463763738407-0.09329552411380.574997480835-0.01665548297330.5901002331351.5830746595-21.99767505946.4678276265
34.67512465117-0.07996000620230.4312656333772.063748799310.005912247320192.15700523267-0.0253023569151-0.108720816501-0.3679249696950.1843001108760.0327845609388-0.1012383834290.207753887003-0.082299147496-0.03599227543130.535943811301-0.03153587986520.005003289809770.308060834962-0.07653146749780.4575491617941.9825657942-19.834443965736.4962694254
42.021286624030.7056592193740.1666338113542.824467320470.3213340595791.470430090590.04816810245870.132614011434-0.255019088293-0.279634266845-0.285719678394-0.01128851548420.211050248536-0.2193327168160.275720017540.598260873184-0.0899276933757-0.003203360815790.499183341309-0.09562200545670.64783396053128.7607738776-32.878957556431.7146505343
51.4093721971-0.658008675339-0.9524795564031.77770553703-0.434721547032.822545419960.02364189845020.1704853961440.136987563757-0.060014259363-0.3477426298490.5835185302770.509836182927-0.2297485215840.1930543245580.743679762358-0.08183653784380.03851982630260.569061168445-0.0903066897360.89646403591528.8154067697-34.487518919435.3239037621
62.77225145501-0.3786474353540.2580773347643.1231975068-0.5143613763871.51291981912-0.154355039791-0.0309485131553-0.5719986715430.7421108371830.2147070204320.9131830078780.216234519008-0.39878142504-0.02655231417750.667807724837-0.01096433022850.1509909498560.610451142125-0.08835460141470.98719078391620.5613662979-35.430075082134.651360519
74.911728687650.9937103807515.642724769433.67153541432-0.8062965950877.6001272471.2563881148-2.4262851177-0.5331569249361.73580123782-0.02106558024610.8593135699521.67269337358-0.271134068206-1.343416567860.993508033611-0.006074610009530.06995468716740.97131970443-0.04388943118970.80367838737423.7501470577-18.481731202246.1096428831
81.92269386826-0.4146818558760.4681592577314.88676297255-0.3831201701742.50680106808-0.06781456169770.0582734329190.167774458151-0.140977639157-0.153644971236-0.957112564983-0.1986871947180.289156938850.2414685996330.713545986081-0.0584629376308-0.03503154947540.7715401571030.06599979144460.66271893064752.8065047402-9.9891191431382.1472783649
91.519281125440.629914068807-0.5312248369993.81125135414-0.2402174613630.8401306989070.0625266974216-0.7145375525420.2672352640850.604260678553-0.0424026317099-0.886100019769-0.4379774194160.2202778869420.02938410284060.968905298744-0.138853152143-0.2008652099860.989506088846-0.009437113942880.73631740158654.2568855353-2.5346895939991.5792454077
102.593549038270.144715303670.3728072525523.40687611166-0.06556225876211.32428560182-0.0810086535713-0.7808825404550.2093816220650.5776592732440.05738062244780.139834665082-0.620233447284-0.191077277226-0.02250748118241.00576212360.08742854796960.002085569752641.02774244255-0.03635641553750.58677974864437.7037836627-5.8859048740895.3814854089
110.8657368914410.4202731005830.3863435438480.3026966028020.1945221996540.160391448723-0.446501631096-0.7004589522210.153876073392-0.3811440148660.06116687318560.383232521718-0.202737615863-0.1647856402990.4099731053821.468398817170.4212821432210.2016795716281.54808377623-0.3868895089751.2004339298120.39842074054.69705148075105.479550236
124.90922541185-0.306339691108-2.321660238654.85261592709-1.215626366135.930757022770.11358772341.717169756810.449502167758-0.07210222911520.9011068805910.92592359026-0.545148246237-0.0661778702774-0.9544841345731.075357920950.149840039076-0.1155755594611.67593874709-0.1444414937051.5559912954520.8344015435-8.070321100387.1291775487
132.69330533398-0.259936458174-1.863072606662.359375570070.5499173492222.15066848605-0.136431740992-0.0756439421304-0.407390609016-0.2014525230590.005968365878980.745527750165-0.263042881497-0.3313022418990.1656796829670.5789637260570.0284336709428-0.06647059800160.8555403355850.07456270235070.75369748102225.0208863266-20.501802545182.3713671337
140.693372203739-0.4633661486691.246775240440.297678132286-0.7419999569823.403173220240.0237846681481-0.2216829128820.131434397714-0.515014195744-0.3678644338140.9154990812970.911437191429-1.562577162670.437347029250.93561355237-0.272037243364-0.1995171533521.32127878493-0.01727965984871.53969783679.76525301966-27.430081938776.1414355904
152.571633857271.15552974238-0.6841260138742.834126083210.1744416599740.508173405364-0.2205373975690.0386027667477-1.45470612786-0.07057554557940.103343700660.7048078097840.211986918865-0.4801950571140.06468090736720.756798271436-0.08448621317520.1004864327971.084782350240.2366094313411.3725061843922.286094373-34.801331546588.8991469627
160.3547513871230.778012694445-0.7933268478742.49948561026-1.224542766312.075567843230.6680749746760.2555954146680.110007541404-0.0530150147714-0.577766613011.175383884630.1405605407040.4155008232210.1047307589780.738183584704-0.175868390775-0.1095992248771.413225297660.08403895009121.6673410676414.3417460701-35.939285878873.3356998069
173.156021884460.02835875570172.115617531070.925298412260.6217427668822.12209063-0.2776318954331.26678612644-0.328276490858-0.828061900550.6333352782560.352637022451-0.7764639447770.0292648455446-0.1946450091161.06877500988-0.0970994129958-0.1472301613971.116261006990.01425485518730.79120918713427.4981559317-23.165587091167.8061187749
183.151763400530.2236601430220.7961216730793.84236690786-1.196693621095.542610369640.02409277761090.165872247409-0.483847391188-1.182773981360.0146663925445-0.2137152622851.249965230360.138213219152-0.2035812309320.926228185426-0.0684867286107-0.02136218735940.7339203448110.02492916893310.97491260082641.5440875102-31.563115272375.4042417633
192.020331425870.326281890136-0.09309644348461.63692030059-0.03907801393111.072751694070.234434511427-1.41640409365-1.191227165150.707246226806-0.1536733784120.05489338414350.491169801857-0.118360164202-0.1554574650280.9772676016580.01160950515690.01218395611271.306125879520.3791267634431.0096780907336.2195980057-33.2052065087101.4261234
202.02896194166-0.552299462192-0.3316255644442.75854249622-0.6122692237361.09457836545-0.107144292031-0.466398535785-1.36911136160.07356006579420.0848656861273-0.4415046061580.6895605571920.2352127597480.0369228769980.964811686873-0.0439265748901-0.003510673225781.038746188180.1478171695081.7048655314351.2228860029-40.445172703686.6001605766
211.379244992230.147453806128-0.7042686498451.81195610101-0.6427468935351.1395877789-0.485996876932-0.5672958040440.307068308201-0.806420400987-0.678027612231-0.4731303713410.8741547964790.4136892751820.9367958902081.514926178580.02616404321070.328749550491.114716754890.008664790557762.5489370882358.6062938813-48.895084864477.6569604564
224.490000388490.728540565874-0.4153558087255.689279638491.115063275615.98930013913-0.2828178805062.338213448840.677531100896-0.690568244132-0.389066298549-0.8669886353550.663100847038-0.4340421995110.3388142951050.805860486274-0.05075730391310.0613089442341.2296623749-0.02161172934541.2424885349153.3893085649-25.11113366975.4250769501
232.21979180871-0.4529355971270.4104008505222.446015794980.3705847446471.22185358449-0.0757400295273-0.244616269349-0.1045428242320.459868077739-0.02666715542810.464859887793-0.00136792515738-0.3098219792790.1155721903910.5774893010660.01744303887680.09722569996650.530124464927-0.02602779867950.42853928287524.4914057096-1.652885868647.3158701458
245.947631962761.05099820061-0.8537328582373.1944767435-0.439342613822.89700034185-0.261882970996-0.04763697794670.78028228233-0.313001754496-0.06880580728230.434073771182-0.713976091919-0.2197956829520.3188111360750.6170554799070.0491289300949-0.08039605024470.4319486093460.03287714590170.50014863043333.358938640812.331455395633.4413816154
251.56257880901-0.436329595714-0.7976558445491.614837193960.08178128641012.69846334130.0757139847219-0.2066903107040.4390272921650.16283514829-0.01140783875331.07832261401-0.394234783718-0.695860497457-0.001684109354490.6195288519420.1458868708590.08165460894080.774758408032-0.03164841290410.96674778784213.94956800958.5273632637440.787620322
263.16656330024-0.06644293368930.2894403800431.873609424280.4857717533872.34826266943-0.178969595405-0.7129376402630.5895108309330.9143068959960.1076534693910.297039122371-0.63660226791-0.3248484482740.1085074012551.061099483780.06251220386350.1121341490620.66359748407-0.08871050554830.65189906474730.24077406549.0613028645658.2532864267
273.57739623885-0.278567294376-0.06218127706042.17767049596-0.04770572907880.52090847360.01289284946730.8846430665890.529514412908-0.386663412051-0.1402299159340.0877746148576-0.1107606018370.04964146217550.1237962480980.577454652752-0.02831183640320.03174637762240.5966870538940.09505940505530.44417400548937.69069288545.8861599010125.7850851607
283.094139308360.1339685610370.8836986004753.72963659796-0.8277623109170.41818437458-0.05081498871840.1506585472730.320788306242-0.02270501803980.10951278834-0.602931099583-0.1291909386920.183074020936-0.04813370762260.48054866125-0.06489376466120.02212596242620.4922085391660.1064816361850.58006094453150.023698971112.411026059531.8530088552
292.155440114121.024473779550.3042627464412.847363523170.01588965945962.702121037850.159039216054-0.2370102637720.5643327893710.0708128276163-0.0859609996346-0.361321434132-0.3326706570920.014895845455-0.2338806663290.8274706309320.009126365334820.009902029032350.605734920553-0.03172843295690.98252064375946.766601972422.769090207137.8471946945
303.609730895140.753405148158-0.01713524093043.35714288721-0.4039984788952.36439775167-0.00676830452429-0.1439417350490.9126417183110.545538522896-0.0326976019829-1.52421397348-0.4926556112550.663334582445-0.001716479979030.760537195048-0.112644369295-0.01408843828280.627864797750.04143534935821.3217944579756.899056978223.467734768136.3409100486
319.681231626562.241023150470.3013986581426.228654280920.5107686448092.2057719625-0.438288366257-2.016586087480.6146051686261.04677875609-1.0082328687-0.369163116864-1.73216500461.468768714661.200862568181.01424084795-0.181679409878-0.1907911103860.9408340205430.006527040618150.90400736091556.54231836964.2593976041944.3954131453
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 114 through 294 or resname ZN)
2X-RAY DIFFRACTION2chain 'A' and (resid 295 through 323 )
3X-RAY DIFFRACTION3chain 'A' and (resid 324 through 449 )
4X-RAY DIFFRACTION4chain 'A' and (resid 450 through 482 )
5X-RAY DIFFRACTION5chain 'A' and (resid 483 through 547 )
6X-RAY DIFFRACTION6chain 'A' and (resid 548 through 583 )
7X-RAY DIFFRACTION7chain 'E' and (resid 801 through 804 )
8X-RAY DIFFRACTION8chain 'B' and (resid 115 through 205 )
9X-RAY DIFFRACTION9chain 'B' and (resid 206 through 323 or resname ZN)
10X-RAY DIFFRACTION10chain 'B' and (resid 324 through 482 )
11X-RAY DIFFRACTION11chain 'B' and (resid 483 through 575 )
12X-RAY DIFFRACTION12chain 'F' and (resid 801 through 804 )
13X-RAY DIFFRACTION13chain 'C' and (resid 114 through 185 )
14X-RAY DIFFRACTION14chain 'C' and (resid 186 through 205 )
15X-RAY DIFFRACTION15chain 'C' and (resid 206 through 273 or resname ZN)
16X-RAY DIFFRACTION16chain 'C' and (resid 274 through 309 )
17X-RAY DIFFRACTION17chain 'C' and (resid 310 through 355 )
18X-RAY DIFFRACTION18chain 'C' and (resid 356 through 393 )
19X-RAY DIFFRACTION19chain 'C' and (resid 394 through 435 )
20X-RAY DIFFRACTION20chain 'C' and (resid 436 through 558 )
21X-RAY DIFFRACTION21chain 'C' and (resid 559 through 579 )
22X-RAY DIFFRACTION22chain 'G' and (resid 801 through 804 )
23X-RAY DIFFRACTION23chain 'D' and (resid 114 through 205 )
24X-RAY DIFFRACTION24chain 'D' and (resid 206 through 232 or resname ZN)
25X-RAY DIFFRACTION25chain 'D' and (resid 233 through 291 )
26X-RAY DIFFRACTION26chain 'D' and (resid 292 through 372 )
27X-RAY DIFFRACTION27chain 'D' and (resid 373 through 436 )
28X-RAY DIFFRACTION28chain 'D' and (resid 437 through 469 )
29X-RAY DIFFRACTION29chain 'D' and (resid 470 through 547 )
30X-RAY DIFFRACTION30chain 'D' and (resid 548 through 583 )
31X-RAY DIFFRACTION31chain 'H' and (resid 801 through 804 )

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