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- PDB-6sl7: The Delta Calcium mutant of ALPHA-ACTININ FROM ENTAMOEBA HISTOLYTICA -
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Open data
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Basic information
Entry | Database: PDB / ID: 6sl7 | |||||||||
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Title | The Delta Calcium mutant of ALPHA-ACTININ FROM ENTAMOEBA HISTOLYTICA | |||||||||
![]() | Calponin homology domain protein putative | |||||||||
![]() | STRUCTURAL PROTEIN / Actinin / Actin Binding Domain / Spectrin Repear / Calmodulin-like domain / Calcium Regulation | |||||||||
Function / homology | ![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Pinotsis, N. / Lopez Arolas, A. / Djinovic-Carugo, K. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Calcium modulates the domain flexibility and function of an alpha-actinin similar to the ancestral alpha-actinin. Authors: Pinotsis, N. / Zielinska, K. / Babuta, M. / Arolas, J.L. / Kostan, J. / Khan, M.B. / Schreiner, C. / Salmazo, A. / Ciccarelli, L. / Puchinger, M. / Gkougkoulia, E.A. / Ribeiro Jr., E.A. / ...Authors: Pinotsis, N. / Zielinska, K. / Babuta, M. / Arolas, J.L. / Kostan, J. / Khan, M.B. / Schreiner, C. / Salmazo, A. / Ciccarelli, L. / Puchinger, M. / Gkougkoulia, E.A. / Ribeiro Jr., E.A. / Marlovits, T.C. / Bhattacharya, A. / Djinovic-Carugo, K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 136 KB | Display | ![]() |
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PDB format | ![]() | 103.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 431.6 KB | Display | ![]() |
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Full document | ![]() | 460.7 KB | Display | |
Data in XML | ![]() | 25.7 KB | Display | |
Data in CIF | ![]() | 34 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5nl6C ![]() 5nl7C ![]() 6sl2SC ![]() 6sl3C C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 69853.633 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.49 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 0.1 M bis-tris (pH 5.5), 0.3 M MgCl2, 20% (w/v) PEG 3,350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Aug 1, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96862 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→46.85 Å / Num. obs: 11494 / % possible obs: 97.8 % / Redundancy: 4.6 % / CC1/2: 0.998 / Rrim(I) all: 0.084 / Net I/σ(I): 11.57 |
Reflection shell | Resolution: 3.3→3.38 Å / Redundancy: 5.8 % / Num. unique obs: 2235 / CC1/2: 0.855 / % possible all: 96.2 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 6SL2 Resolution: 3.3→46.32 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 3.3→46.32 Å
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