modulation of formation of structure involved in a symbiotic process / rRNA (cytosine-N4-)-methyltransferase activity / ATP-activated inward rectifier potassium channel activity / kinetoplast / quorum sensing / response to arsenic-containing substance / rRNA base methylation / ciliary plasm / mitochondrial small ribosomal subunit / acyl binding ...modulation of formation of structure involved in a symbiotic process / rRNA (cytosine-N4-)-methyltransferase activity / ATP-activated inward rectifier potassium channel activity / kinetoplast / quorum sensing / response to arsenic-containing substance / rRNA base methylation / ciliary plasm / mitochondrial small ribosomal subunit / acyl binding / acyl carrier activity / iron-sulfur cluster binding / voltage-gated potassium channel activity / voltage-gated potassium channel complex / 転移酵素; 一炭素原子の基を移すもの; メチル基を移すもの / methyltransferase activity / protein homooligomerization / potassium ion transport / fatty acid biosynthetic process / structural constituent of ribosome / mitochondrial matrix / translation / mRNA binding / mitochondrion / metal ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能
T-cell activation inhibitor, mitochondrial / Domain of unknown function DUF4460 / Domain of unknown function (DUF4460) / Probable Zinc-ribbon domain / Probable Treble clef zinc finger domain / Ribosomal protein Rsm22-like / : / Mitochondrial small ribosomal subunit Rsm22 / Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily ...T-cell activation inhibitor, mitochondrial / Domain of unknown function DUF4460 / Domain of unknown function (DUF4460) / Probable Zinc-ribbon domain / Probable Treble clef zinc finger domain / Ribosomal protein Rsm22-like / : / Mitochondrial small ribosomal subunit Rsm22 / Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily / MraW methylase family / Small ribosomal subunit protein mS41 SAM domain / Protein Fyv4 / IGR protein motif / IGR / Phosphotyrosine protein phosphatase I / Phosphotyrosine protein phosphatase I superfamily / Low molecular weight phosphotyrosine protein phosphatase / Low molecular weight phosphatase family / Pentatricopeptide repeat domain / Potassium channel tetramerisation-type BTB domain / BTB/POZ domain / Pentatricopeptide (PPR) repeat profile. / Pentatricopeptide repeat / Acyl carrier protein (ACP) / Phosphopantetheine attachment site / SKP1/BTB/POZ domain superfamily / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Translation elongation factor EF1B/ribosomal protein S6 / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S11 superfamily / S15/NS1, RNA-binding / Thioredoxin-like superfamily / S-adenosyl-L-methionine-dependent methyltransferase superfamily 類似検索 - ドメイン・相同性
Chem-PM8 / PHOSPHATE ION / RNA / RNA (> 10) / RNA (> 100) / Uncharacterized protein / Uncharacterized protein / Methyltransferase / Uncharacterized protein / Potassium voltage-gated channel, putative ...Chem-PM8 / PHOSPHATE ION / RNA / RNA (> 10) / RNA (> 100) / Uncharacterized protein / Uncharacterized protein / Methyltransferase / Uncharacterized protein / Potassium voltage-gated channel, putative / Uncharacterized protein / Small ribosomal subunit protein mS41 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Target SNARE / Phosphotyrosine protein phosphatase I domain-containing protein / DUF4460 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Uncharacterized protein / Glutaredoxin domain-containing protein / S-adenosyl-methyltransferase mraW-like protein, putative / Acyl carrier protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Treble clef zinc finger domain-containing protein / Succinate dehydrogenase assembly factor 3, mitochondrial / Uncharacterized protein 類似検索 - 構成要素
ジャーナル: Science / 年: 2019 タイトル: Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery. 著者: Martin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut / ...著者: Martin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut / Daniel Boehringer / André Schneider / Nenad Ban / 要旨: Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome ...Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.
分子量: 50147.355 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#2: タンパク質
bS6m
分子量: 18861.543 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q38BW5
#3: タンパク質
uS8m
分子量: 32766.459 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#4: タンパク質
uS11m
分子量: 37953.965 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q389T7
#5: タンパク質
uS15m
分子量: 50401.191 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q4GZ99
#6: タンパク質
bS16m
分子量: 22077.805 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q384N9
#7: タンパク質
uS17m
分子量: 39012.414 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; C-terminal PTP-tag 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#8: タンパク質
bS18m
分子量: 36988.691 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#9: タンパク質
mS22
分子量: 73064.141 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q38DR3
#10: タンパク質
mS23
分子量: 35358.805 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#11: タンパク質
mS26
分子量: 51136.684 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q38DK6
#12: タンパク質
mS34
分子量: 28728.180 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q57UK0
#13: タンパク質
mS38
分子量: 28240.600 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q57VQ9
#14: タンパク質
mS41
分子量: 22421.469 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q389L3
#15: タンパク質
mS51 (KRIPP1)
分子量: 95080.992 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#16: タンパク質
mS56
分子量: 47306.836 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#17: タンパク質
mS59
分子量: 35395.676 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#18: タンパク質
mS62 (KRIPP14)
分子量: 32068.100 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q383D1
#19: タンパク質
mS63 (KRIPP16)
分子量: 31922.178 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#20: タンパク質
mS65
分子量: 30617.381 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#21: タンパク質
mS68
分子量: 26472.807 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#22: タンパク質
mS73
分子量: 11360.885 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q587C4
#23: タンパク質
mt-SAF2 (KRIPP2)
分子量: 116189.547 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#24: タンパク質
mt-SAF3
分子量: 106621.727 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q38E61
#25: タンパク質
mt-SAF5
分子量: 86949.414 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#26: タンパク質
mt-SAF6
分子量: 76781.398 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q38FQ8
#27: タンパク質
mt-SAF7 (KRIPP10)
分子量: 76675.156 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q57UW6
#28: タンパク質
mt-SAF8
分子量: 76725.180 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#29: タンパク質
mt-SAF9
分子量: 69781.789 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#30: タンパク質
mt-SAF10
分子量: 69306.758 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q386U1
#31: タンパク質
mt-SAF11
分子量: 64916.957 Da / 分子数: 2 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#32: タンパク質
mt-SAF13
分子量: 63690.703 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#33: タンパク質
mt-SAF18
分子量: 42508.039 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#34: タンパク質
mt-SAF21
分子量: 39610.793 Da / 分子数: 2 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#35: タンパク質
mt-SAF22 (KRIPP17)
分子量: 39132.621 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q389F9
#36: タンパク質
mt-SAF23
分子量: 38240.164 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#37: タンパク質
mt-SAF24
分子量: 34648.047 Da / 分子数: 5 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q387P8
#38: タンパク質
mt-SAF26
分子量: 30538.557 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q38FI3
#39: タンパク質
mt-SAF27
分子量: 26936.748 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q57YK5
#40: タンパク質
mt-SAF28
分子量: 21468.707 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#41: タンパク質
mt-SAF29
分子量: 20048.514 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427
#42: タンパク質
mt-SAF30
分子量: 19659.756 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q57VU7
#43: タンパク質
mt-SAF31
分子量: 17673.535 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q581U4
#44: タンパク質
mt-SAF32
分子量: 16550.807 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q57WW9
#45: タンパク質
mt-SAF33
分子量: 16291.121 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q581T4
分子量: 119799.398 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q385R2
#62: タンパク質
mt-SAF14
分子量: 53320.820 Da / 分子数: 1 / 由来タイプ: 天然 詳細: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence 由来: (天然) Trypanosoma brucei brucei (トリパノソーマ) 株: Lister strain 427 / 参照: UniProt: Q57W60
包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES 詳細: Body domain of the mitochondrial ribosomal small subunit assemblosome of Trypanosoma brucei brucei Lister strain 427
電子線照射量: 40 e/Å2 / 検出モード: INTEGRATING フィルム・検出器のモデル: FEI FALCON III (4k x 4k)
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解析
EMソフトウェア
ID
名称
カテゴリ
2
EPU
画像取得
4
Gctf
CTF補正
9
RELION
初期オイラー角割当
10
RELION
最終オイラー角割当
12
RELION
3次元再構成
CTF補正
詳細: On the fly in RELION / タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
対称性
点対称性: C1 (非対称)
3次元再構成
解像度: 3.1 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 161661 / アルゴリズム: FOURIER SPACE / 対称性のタイプ: POINT
原子モデル構築
プロトコル: OTHER 詳細: Starting models of ribosomal proteins of the T. brucei mitochondrial ribosomal small subunit were taken from PDB 6HIW. Mitchondrial ribosomal small subunit assembly factors were built de novo.