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- PDB-6sf2: Ternary complex of human bone morphogenetic protein 9 (BMP9) grow... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6sf2 | ||||||||||||
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Title | Ternary complex of human bone morphogenetic protein 9 (BMP9) growth factor domain, its prodomain and extracellular domain of activin receptor-like kinase 1 (ALK1). | ||||||||||||
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![]() | CYTOKINE / BMP9 / ALK1 / prodomain / ternary complex / signalling / TGFbeta / BMP | ||||||||||||
Function / homology | ![]() lymphatic endothelial cell differentiation / regulation of endothelial cell proliferation / negative regulation of endothelial cell differentiation / dorsal aorta morphogenesis / positive regulation of epithelial cell differentiation / blood vessel maturation / venous blood vessel development / positive regulation of cartilage development / transforming growth factor beta receptor activity / lymphangiogenesis ...lymphatic endothelial cell differentiation / regulation of endothelial cell proliferation / negative regulation of endothelial cell differentiation / dorsal aorta morphogenesis / positive regulation of epithelial cell differentiation / blood vessel maturation / venous blood vessel development / positive regulation of cartilage development / transforming growth factor beta receptor activity / lymphangiogenesis / positive regulation of chondrocyte differentiation / BMP receptor complex / retina vasculature development in camera-type eye / BMP receptor activity / blood vessel endothelial cell proliferation involved in sprouting angiogenesis / positive regulation of endothelial cell differentiation / activin receptor activity, type I / transforming growth factor beta receptor activity, type I / negative regulation of focal adhesion assembly / endothelial tube morphogenesis / positive regulation of bicellular tight junction assembly / regulation of blood vessel endothelial cell migration / artery development / receptor protein serine/threonine kinase / transmembrane receptor protein serine/threonine kinase activity / Signaling by BMP / activin binding / cellular response to BMP stimulus / activin receptor signaling pathway / positive regulation of BMP signaling pathway / negative regulation of cell adhesion / transforming growth factor beta binding / dorsal/ventral pattern formation / blood circulation / cartilage development / wound healing, spreading of epidermal cells / blood vessel morphogenesis / negative regulation of endothelial cell migration / endocardial cushion morphogenesis / branching involved in blood vessel morphogenesis / positive regulation of Notch signaling pathway / negative regulation of DNA replication / regulation of DNA replication / SMAD binding / negative regulation of endothelial cell proliferation / positive regulation of SMAD protein signal transduction / negative regulation of blood vessel endothelial cell migration / blood vessel remodeling / vasculogenesis / BMP signaling pathway / cellular response to transforming growth factor beta stimulus / positive regulation of endothelial cell proliferation / protein serine/threonine kinase activator activity / negative regulation of angiogenesis / negative regulation of cell migration / ossification / transforming growth factor beta receptor signaling pathway / cytokine activity / positive regulation of interleukin-8 production / growth factor activity / negative regulation of cell growth / cellular response to growth factor stimulus / regulation of blood pressure / osteoblast differentiation / positive regulation of angiogenesis / heart development / angiogenesis / intracellular iron ion homeostasis / in utero embryonic development / transcription by RNA polymerase II / response to hypoxia / negative regulation of cell population proliferation / phosphorylation / negative regulation of gene expression / protein serine/threonine kinase activity / neuronal cell body / dendrite / regulation of DNA-templated transcription / protein kinase binding / positive regulation of DNA-templated transcription / cell surface / signal transduction / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular exosome / extracellular region / ATP binding / metal ion binding / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Salmon, R.M. / Guo, J. / Yu, M. / Li, W. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Molecular basis of ALK1-mediated signalling by BMP9/BMP10 and their prodomain-bound forms. Authors: Salmon, R.M. / Guo, J. / Wood, J.H. / Tong, Z. / Beech, J.S. / Lawera, A. / Yu, M. / Grainger, D.J. / Reckless, J. / Morrell, N.W. / Li, W. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 283.5 KB | Display | ![]() |
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PDB format | ![]() | 189.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 485.9 KB | Display | ![]() |
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Full document | ![]() | 488.2 KB | Display | |
Data in XML | ![]() | 21.9 KB | Display | |
Data in CIF | ![]() | 29.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6sf1C ![]() 6sf3C ![]() 4faoS ![]() 4ycgS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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Components
#1: Protein | Mass: 10788.126 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P37023, receptor protein serine/threonine kinase #2: Protein | Mass: 12102.971 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #3: Protein | Mass: 33104.816 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #4: Sugar | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.68 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / Details: 0.14 M potassium sodium tartrate, 14% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Ambient temp details: 100K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 10, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9282 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→62.88 Å / Num. obs: 19622 / % possible obs: 100 % / Redundancy: 9.9 % / Biso Wilson estimate: 90.13 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.154 / Rpim(I) all: 0.052 / Rrim(I) all: 0.163 / Net I/σ(I): 13.3 |
Reflection shell | Resolution: 3.3→3.48 Å / Redundancy: 10.2 % / Mean I/σ(I) obs: 2.8 / Num. unique obs: 2834 / CC1/2: 0.779 / Rpim(I) all: 0.369 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4FAO, 4YCG Resolution: 3.3→62.25 Å / SU ML: 0.4924 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 28.5581 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 89.58 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.3→62.25 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -23.2435955183 Å / Origin y: -20.3457557922 Å / Origin z: -11.4726290322 Å
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Refinement TLS group | Selection details: all |