温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6 詳細: Protein dissolved in 50 mM MES pH 6.0, 45% (v/v) DMSO to a concentration of 100 mg/ ml. Equal amounts of the protein solution and a solution containing 50 mM MES pH 6.0, 1.0 M NaCl, 45% (v/v) ...詳細: Protein dissolved in 50 mM MES pH 6.0, 45% (v/v) DMSO to a concentration of 100 mg/ ml. Equal amounts of the protein solution and a solution containing 50 mM MES pH 6.0, 1.0 M NaCl, 45% (v/v) DMSO were mixed and equilibrated over a well containing 35% (v/v) saturated solution of ammonium sulphate.
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平均測定温度: 293 K Ambient temp details: in situ experiment at room temperature Serial crystal experiment: N
解像度: 2.2→81.02 Å / SU ML: 0.1677 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 15.5475 詳細: Data collected on three crystals were merged and reprocessed resulting in slightly better statistics (possibly due to different parameters used in processing). These merged data were used for ...詳細: Data collected on three crystals were merged and reprocessed resulting in slightly better statistics (possibly due to different parameters used in processing). These merged data were used for molecular replacement and were then refined.