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- PDB-6rtu: Piperideine-6-carboxylate dehydrogenase from Streptomyces clavuli... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rtu | |||||||||
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Title | Piperideine-6-carboxylate dehydrogenase from Streptomyces clavuligerus complexed with alpha-aminoadipic acid | |||||||||
![]() | Semialdehyde dehydrogenase Pcd | |||||||||
![]() | OXIDOREDUCTASE / antibiotic biosynthesis / Streptomyces clavuligerus | |||||||||
Function / homology | ![]() glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity / aldehyde dehydrogenase (NAD+) / aldehyde dehydrogenase (NAD+) activity / nucleotide binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Hasse, D. / Huelsemann, J. / Carlsson, G. / Andersson, I. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and mechanism of piperideine-6-carboxylate dehydrogenase from Streptomyces clavuligerus. Authors: Hasse, D. / Hulsemann, J. / Carlsson, G.H. / Valegard, K. / Andersson, I. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 218.2 KB | Display | ![]() |
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PDB format | ![]() | 171 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 726 KB | Display | ![]() |
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Full document | ![]() | 729.6 KB | Display | |
Data in XML | ![]() | 42.6 KB | Display | |
Data in CIF | ![]() | 64.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6rtrSC ![]() 6rtsC ![]() 6rttC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 54088.996 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: pcd / Production host: ![]() ![]() |
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-Non-polymers , 5 types, 784 molecules ![](data/chem/img/UN1.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-UN1 / | ||||||
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#3: Chemical | ChemComp-SO4 / #4: Chemical | ChemComp-GOL / #5: Chemical | ChemComp-ACT / | #6: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.71 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 0.1 M sodium acetate pH 5, 2.2 M ammonium sulphate, 0.2 M lithium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 7, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→45.6 Å / Num. obs: 89138 / % possible obs: 97.9 % / Redundancy: 3.5 % / Biso Wilson estimate: 24 Å2 / Rrim(I) all: 0.086 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 1.9→1.95 Å / Redundancy: 3.4 % / Mean I/σ(I) obs: 1.45 / Num. unique obs: 7528 / Rrim(I) all: 0.721 / % possible all: 86.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6RTR Resolution: 1.9→45.575 Å / SU ML: 0.2 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 19.87
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→45.575 Å
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Refine LS restraints |
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LS refinement shell |
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