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- PDB-6rsy: The complex between TCR a7b2 and human Class I MHC HLA-A0201-WT1 ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rsy | ||||||
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Title | The complex between TCR a7b2 and human Class I MHC HLA-A0201-WT1 with the bound RMFPNAPYL peptide. | ||||||
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![]() | IMMUNE SYSTEM / MHC CLASS I ANTIGEN- TCR - peptide complex | ||||||
Function / homology | ![]() posterior mesonephric tubule development / negative regulation of metanephric glomerular mesangial cell proliferation / positive regulation of metanephric ureteric bud development / thorax and anterior abdomen determination / metanephric epithelium development / regulation of animal organ formation / adrenal cortex formation / negative regulation of female gonad development / positive regulation of heart growth / visceral serous pericardium development ...posterior mesonephric tubule development / negative regulation of metanephric glomerular mesangial cell proliferation / positive regulation of metanephric ureteric bud development / thorax and anterior abdomen determination / metanephric epithelium development / regulation of animal organ formation / adrenal cortex formation / negative regulation of female gonad development / positive regulation of heart growth / visceral serous pericardium development / Nephron development / glomerular basement membrane development / diaphragm development / sex determination / mesenchymal to epithelial transition / metanephric mesenchyme development / metanephric S-shaped body morphogenesis / positive regulation of male gonad development / cellular response to gonadotropin stimulus / Transcriptional regulation of testis differentiation / gonad development / podocyte differentiation / cardiac muscle cell fate commitment / double-stranded methylated DNA binding / hemi-methylated DNA-binding / tissue development / male genitalia development / glomerulus development / camera-type eye development / C2H2 zinc finger domain binding / ureteric bud development / negative regulation of gene expression via chromosomal CpG island methylation / T cell mediated cytotoxicity directed against tumor cell target / positive regulation of memory T cell activation / TAP complex binding / branching involved in ureteric bud morphogenesis / adrenal gland development / positive regulation of CD8-positive, alpha-beta T cell activation / CD8-positive, alpha-beta T cell activation / Golgi medial cisterna / positive regulation of CD8-positive, alpha-beta T cell proliferation / CD8 receptor binding / antigen processing and presentation of exogenous peptide antigen via MHC class I / germ cell development / endoplasmic reticulum exit site / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent / TAP binding / protection from natural killer cell mediated cytotoxicity / vasculogenesis / beta-2-microglobulin binding / T cell receptor binding / detection of bacterium / cellular response to cAMP / epithelial cell differentiation / RNA splicing / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / kidney development / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / lumenal side of endoplasmic reticulum membrane / cellular response to iron ion / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / regulation of erythrocyte differentiation / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of iron ion transport / response to molecule of bacterial origin / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / negative regulation of cell growth / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / positive regulation of miRNA transcription / negative regulation of neurogenesis / peptide antigen assembly with MHC class II protein complex / positive regulation of receptor-mediated endocytosis / MHC class II protein complex / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / positive regulation of cellular senescence / peptide antigen binding / negative regulation of epithelial cell proliferation / antigen processing and presentation of exogenous peptide antigen via MHC class II / male gonad development Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Srikannathasan, V. / Robinson, R.A. | ||||||
![]() | ![]() Title: Specificity of bispecific T cell receptors and antibodies targeting peptide-HLA. Authors: Holland, C.J. / Crean, R.M. / Pentier, J.M. / de Wet, B. / Lloyd, A. / Srikannathasan, V. / Lissin, N. / Lloyd, K.A. / Blicher, T.H. / Conroy, P.J. / Hock, M. / Pengelly, R.J. / Spinner, T.E. ...Authors: Holland, C.J. / Crean, R.M. / Pentier, J.M. / de Wet, B. / Lloyd, A. / Srikannathasan, V. / Lissin, N. / Lloyd, K.A. / Blicher, T.H. / Conroy, P.J. / Hock, M. / Pengelly, R.J. / Spinner, T.E. / Cameron, B. / Potter, E.A. / Jeyanthan, A. / Molloy, P.E. / Sami, M. / Aleksic, M. / Liddy, N. / Robinson, R.A. / Harper, S. / Lepore, M. / Pudney, C.R. / van der Kamp, M.W. / Rizkallah, P.J. / Jakobsen, B.K. / Vuidepot, A. / Cole, D.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 337.8 KB | Display | ![]() |
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PDB format | ![]() | 278.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 61.5 KB | Display | |
Data in CIF | ![]() | 83.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
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Components
-Protein , 4 types, 8 molecules AFBGDIEJ
#1: Protein | Mass: 31951.316 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Homo sapiens / Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 11879.356 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: BETA-2-MICROGLOBULIN / Source: (gene. exp.) ![]() ![]() ![]() #4: Protein | Mass: 23081.580 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: few N-terminal residues missing in the structure and also C-terminal residues. Source: (gene. exp.) ![]() ![]() ![]() #5: Protein | Mass: 28074.318 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Protein/peptide , 1 types, 2 molecules CH
#3: Protein/peptide | Mass: 1109.320 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Peptide, synthesized / Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 2 types, 159 molecules ![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#6: Chemical | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53.02 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 7.5% PEG 8 K , 0.75 M NaCl , 50mM HEPES , pH 7.0 |
-Data collection
Diffraction | Mean temperature: 293 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 17, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9159 Å / Relative weight: 1 |
Reflection | Resolution: 2.95→114.75 Å / Num. obs: 43245 / % possible obs: 100 % / Redundancy: 14.1 % / Rmerge(I) obs: 0.346 / Net I/σ(I): 8.8 |
Reflection shell | Resolution: 2.95→3 Å / Redundancy: 14 % / Rmerge(I) obs: 2.016 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 43245 / CC1/2: 0.524 / % possible all: 99.95 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 73.426 Å2
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Refinement step | Cycle: 1 / Resolution: 2.95→97.76 Å
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Refine LS restraints |
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