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Open data
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Basic information
Entry | Database: PDB / ID: 6rpj | ||||||||||||
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Title | A Non-blocking anti-CTLA-4 Nanobody complexed with CTLA-4 | ||||||||||||
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![]() | IMMUNE SYSTEM / Antibody / check-point / CTLA-4 / receptor | ||||||||||||
Function / homology | ![]() protein complex involved in cell adhesion / negative regulation of regulatory T cell differentiation / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / clathrin-coated endocytic vesicle / CTLA4 inhibitory signaling / negative regulation of B cell proliferation / negative regulation of T cell proliferation / B cell receptor signaling pathway / T cell receptor signaling pathway / adaptive immune response ...protein complex involved in cell adhesion / negative regulation of regulatory T cell differentiation / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / clathrin-coated endocytic vesicle / CTLA4 inhibitory signaling / negative regulation of B cell proliferation / negative regulation of T cell proliferation / B cell receptor signaling pathway / T cell receptor signaling pathway / adaptive immune response / immune response / positive regulation of apoptotic process / external side of plasma membrane / DNA damage response / perinuclear region of cytoplasm / Golgi apparatus / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Gao, H. / Zhou, A. / Zhang, F. | ||||||||||||
![]() | ![]() Title: A Non-blocking anti-CTLA-4 Nanobody complexed with CTLA-4 Authors: Gao, H. / Zhou, A. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 376.7 KB | Display | ![]() |
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PDB format | ![]() | 323.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 912.9 KB | Display | ![]() |
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Full document | ![]() | 909.2 KB | Display | |
Data in XML | ![]() | 32.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein / Antibody , 2 types, 8 molecules ACEGBDFH
#1: Protein | Mass: 13893.714 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Antibody | Mass: 15203.618 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 2 types, 7 molecules ![](data/chem/img/NAG.gif)
#3: Polysaccharide | #4: Sugar | ChemComp-NAG / |
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-Non-polymers , 2 types, 4 molecules ![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.61 Å3/Da / Density % sol: 73.29 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 R 300K / Detector: PIXEL / Date: Nov 11, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 |
Reflection | Resolution: 3.25→50 Å / Num. obs: 30159 / % possible obs: 100 % / Redundancy: 4.7 % / Rmerge(I) obs: 0.146 / Net I/σ(I): 8 |
Reflection shell | Resolution: 3.25→3.37 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.718 / Mean I/σ(I) obs: 3 / Num. unique obs: 1926 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 89.735 Å2
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Refinement step | Cycle: LAST / Resolution: 3.25→47.57 Å
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Refine LS restraints |
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