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- PDB-6qvt: CMP-Sialic acid bound structure of the human wild type Beta-galac... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6qvt | ||||||
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Title | CMP-Sialic acid bound structure of the human wild type Beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal1) | ||||||
![]() | Beta-galactoside alpha-2,6-sialyltransferase 1 | ||||||
![]() | TRANSFERASE / Sialyltransferase / Beta-galactoside alpha-2 / 6-sialyltransferase 1 / ST6Gal1 / N-linked glycosylation / CMP-Sia / Sialic acid / Cytidine-5'-monophospho-N-acetylneuraminic / CMP-Neu5Ac / CMP-Sialic acid | ||||||
Function / homology | ![]() beta-galactoside alpha-(2,6)-sialyltransferase / beta-galactoside alpha-2,6-sialyltransferase activity / Maturation of protein 3a / sialyltransferase activity / N-acetylneuraminate metabolic process / sialylation / Maturation of protein 3a / N-Glycan antennae elongation / Golgi trans cisterna / Termination of O-glycan biosynthesis ...beta-galactoside alpha-(2,6)-sialyltransferase / beta-galactoside alpha-2,6-sialyltransferase activity / Maturation of protein 3a / sialyltransferase activity / N-acetylneuraminate metabolic process / sialylation / Maturation of protein 3a / N-Glycan antennae elongation / Golgi trans cisterna / Termination of O-glycan biosynthesis / regulation of substrate adhesion-dependent cell spreading / O-glycan processing / negative regulation of chemotaxis / negative regulation of macrophage apoptotic process / Sialic acid metabolism / positive regulation of mononuclear cell proliferation / protein N-linked glycosylation via asparagine / Golgi cisterna membrane / Golgi medial cisterna / humoral immune response / response to ethanol / Maturation of spike protein / viral protein processing / Golgi membrane / Golgi apparatus / protein homodimerization activity / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Harrus, D. / Glumoff, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Unliganded and CMP-Neu5Ac bound structures of human alpha-2,6-sialyltransferase ST6Gal I at high resolution. Authors: Harrus, D. / Harduin-Lepers, A. / Glumoff, T. #1: ![]() Title: Unliganded and CMP-Neu5Ac bound structures of human alpha-2,6-sialyltransferase ST6Gal I at high resolution Authors: Harrus, D. / Harduin-Lepers, A. / Glumoff, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 133.6 KB | Display | ![]() |
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PDB format | ![]() | 103.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.9 MB | Display | ![]() |
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Full document | ![]() | 1.9 MB | Display | |
Data in XML | ![]() | 25.4 KB | Display | |
Data in CIF | ![]() | 35.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6qvsC ![]() 4js1S S: Starting model for refinement C: citing same article ( |
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Similar structure data | |
Experimental dataset #1 | Data reference: ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 31803.148 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Compound details: residues 365-371 (chain A) and 132-135, 366-371 (chain B) did not have clear electron density and were not traced Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P15907, beta-galactoside alpha-(2,6)-sialyltransferase #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.21 Å3/Da / Density % sol: 61.62 % / Description: 30-100 um prism-shaped plates |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 0.1 M Bis-Tris pH 5.5, 0.1 M Ammonium Acetate, 10% (w/v) PEG 6000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 27, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976254 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→48.346 Å / Num. obs: 104377 / % possible obs: 98.73 % / Redundancy: 3.4 % / Rmerge(I) obs: 0.0419 / Rpim(I) all: 0.02626 / Rrim(I) all: 0.04958 / Net I/σ(I): 13.87 |
Reflection shell | Resolution: 1.7→1.761 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.6138 / Mean I/σ(I) obs: 1.1 / Num. unique obs: 10330 / Rpim(I) all: 0.3763 / Rrim(I) all: 0.7214 / % possible all: 97.78 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4JS1 Resolution: 1.7→48.346 Å / SU ML: 0.17 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 23.13 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 99.38 Å2 / Biso mean: 35.2105 Å2 / Biso min: 15.48 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.7→48.346 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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