+Open data
-Basic information
Entry | Database: PDB / ID: 6qph | |||||||||
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Title | Dunaliella minimal PSI complex | |||||||||
Components |
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Keywords | PHOTOSYNTHESIS / Photosystem I / Dunaliella / Electron Transport | |||||||||
Function / homology | Function and homology information photosynthesis, light harvesting / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chlorophyll binding / chloroplast thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity ...photosynthesis, light harvesting / photosystem I / photosynthetic electron transport in photosystem I / photosystem I / photosystem II / chlorophyll binding / chloroplast thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / magnesium ion binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Dunaliella salina (plant) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.4 Å | |||||||||
Authors | Klaiman, D. / Caspy, I. / Nelson, N. | |||||||||
Funding support | Israel, 2items
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Citation | Journal: Nat Plants / Year: 2020 Title: Structure of a minimal photosystem I from the green alga Dunaliella salina. Authors: Annemarie Perez-Boerema / Daniel Klaiman / Ido Caspy / Sigal Y Netzer-El / Alexey Amunts / Nathan Nelson / Abstract: Solar energy harnessed by oxygenic photosynthesis supports most of the life forms on Earth. In eukaryotes, photosynthesis occurs in chloroplasts and is achieved by membrane-embedded macromolecular ...Solar energy harnessed by oxygenic photosynthesis supports most of the life forms on Earth. In eukaryotes, photosynthesis occurs in chloroplasts and is achieved by membrane-embedded macromolecular complexes that contain core and peripheral antennae with multiple pigments. The structure of photosystem I (PSI) comprises the core and light-harvesting (LHCI) complexes, which together form PSI-LHCI. Here we determined the structure of PSI-LHCI from the salt-tolerant green alga Dunaliella salina using X-ray crystallography and electron cryo-microscopy. Our results reveal a previously undescribed configuration of the PSI core. It is composed of only 7 subunits, compared with 14-16 subunits in plants and the alga Chlamydomonas reinhardtii, and forms the smallest known PSI. The LHCI is poorly conserved at the sequence level and binds to pigments that form new energy pathways, and the interactions between the individual Lhca1-4 proteins are weakened. Overall, the data indicate the PSI of D. salina represents a different type of the molecular organization that provides important information for reconstructing the plasticity and evolution of PSI. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6qph.cif.gz | 1.6 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6qph.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 6qph.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qp/6qph ftp://data.pdbj.org/pub/pdb/validation_reports/qp/6qph | HTTPS FTP |
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-Related structure data
Related structure data | 4883C 6rhzC 5l8rS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Chlorophyll a-b binding protein, ... , 2 types, 2 molecules 13
#1: Protein | Mass: 21149.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: C1K003 |
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#3: Protein | Mass: 22732.824 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: C1K004 |
-Protein , 5 types, 5 molecules 24DEF
#2: Protein | Mass: 23120.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) |
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#4: Protein | Mass: 23131.031 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) |
#8: Protein | Mass: 15989.417 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) |
#9: Protein | Mass: 7297.158 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) |
#10: Protein | Mass: 18212.902 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) |
-Photosystem I ... , 4 types, 4 molecules ABCJ
#5: Protein | Mass: 81748.172 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXV2, photosystem I |
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#6: Protein | Mass: 81742.516 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXZ0, photosystem I |
#7: Protein | Mass: 8717.090 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXW7, photosystem I |
#11: Protein/peptide | Mass: 4614.362 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXW0 |
-Sugars , 2 types, 5 molecules
#21: Sugar | ChemComp-DGD / #22: Sugar | ChemComp-LMT / | |
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-Non-polymers , 14 types, 198 molecules
#12: Chemical | ChemComp-LUT / ( #13: Chemical | ChemComp-XAT / ( #14: Chemical | ChemComp-BCR / #15: Chemical | ChemComp-CLA / #16: Chemical | ChemComp-CHL / #17: Chemical | ChemComp-LHG / #18: Chemical | ChemComp-LMG / #19: Chemical | ChemComp-SQD / | #20: Chemical | ChemComp-CAC / #23: Chemical | #24: Chemical | ChemComp-CL0 / | #25: Chemical | #26: Chemical | #27: Chemical | ChemComp-CA / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53.2 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6.5 / Details: Sodium cacodylate 50mM, MgCl2 100mM, PEG1500 8-11% |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 0.999 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 8, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.999 Å / Relative weight: 1 |
Reflection | Resolution: 3.4→48.947 Å / Num. obs: 83250 / % possible obs: 99.9 % / Redundancy: 8.3 % / Biso Wilson estimate: 109.15 Å2 / Rpim(I) all: 0.08 / Net I/σ(I): 8.2 |
Reflection shell | Resolution: 3.4→3.5 Å / Num. unique obs: 4575 / Rpim(I) all: 0.636 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5L8R Resolution: 3.4→48.947 Å / SU ML: 0.69 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 45.94
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.4→48.947 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 35.3531 Å / Origin y: 1.1142 Å / Origin z: 44.127 Å
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Refinement TLS group | Selection details: all |