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Open data
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Basic information
Entry | Database: PDB / ID: 6p8r | ||||||
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Title | Structure of P. aeruginosa ATCC27853 HORMA2 | ||||||
![]() | HORMA domain containing protein | ||||||
![]() | PROTEIN BINDING / HORMA domain / CD-NTase / cGAS | ||||||
Function / homology | Bacterial HORMA domain / Bacterial HORMA domain 2 / HORMA domain superfamily / defense response to virus / PHOSPHATE ION / CD-NTase-associated protein 7 / HORMA domain containing protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ye, Q. / Corbett, K.D. | ||||||
![]() | ![]() Title: HORMA Domain Proteins and a Trip13-like ATPase Regulate Bacterial cGAS-like Enzymes to Mediate Bacteriophage Immunity. Authors: Ye, Q. / Lau, R.K. / Mathews, I.T. / Birkholz, E.A. / Watrous, J.D. / Azimi, C.S. / Pogliano, J. / Jain, M. / Corbett, K.D. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 273.2 KB | Display | ![]() |
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PDB format | ![]() | 224.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.6 KB | Display | ![]() |
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Full document | ![]() | 456.7 KB | Display | |
Data in XML | ![]() | 25.5 KB | Display | |
Data in CIF | ![]() | 34.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6p80C ![]() 6p82C ![]() 6p8jC ![]() 6p8oC ![]() 6p8pC ![]() 6p8sC ![]() 6p8uC ![]() 6p8vC ![]() 6pb3C ![]() 6u7bC C: citing same article ( |
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Similar structure data | |
Experimental dataset #1 | Data set type: diffraction image data / Details: Native diffraction dataset / Metadata reference: 10.15785/SBGRID/676 |
Experimental dataset #2 | Data set type: diffraction image data Details: Selenomethionine anomalous SAD dataset used to determine the structure Metadata reference: 10.15785/SBGRID/677 |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18864.143 Da / Num. of mol.: 4 / Mutation: N-terminal SNA Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: ORF C60, CAZ10_14260, DY940_15620, DY979_07580, EGY23_20890, EQH76_12140, IPC669_24875, PA5486_02901, PAERUG_E15_London_28_01_14_04350, PAMH19_6113 Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.91 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 100 mM Imidazole pH 8.0, 0.8 M NaH2PO4, and 0.55-0.8 M KH2PO4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 29, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.14→106.11 Å / Num. obs: 45579 / % possible obs: 99.3 % / Redundancy: 6.6 % / CC1/2: 0.999 / Rmerge(I) obs: 0.064 / Rpim(I) all: 0.028 / Rrim(I) all: 0.07 / Net I/σ(I): 22.6 |
Reflection shell | Resolution: 2.14→2.2 Å / Redundancy: 6.5 % / Rmerge(I) obs: 1.402 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 3448 / CC1/2: 0.516 / Rpim(I) all: 0.588 / Rrim(I) all: 1.524 / % possible all: 91.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.141→61.13 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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