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Open data
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Basic information
Entry | Database: PDB / ID: 5eea | ||||||
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Title | Structure of HOXB13-DNA(CAA) complex | ||||||
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![]() | TRANSCRIPTION / transcription factor / DNA / complex | ||||||
Function / homology | ![]() epithelial cell maturation involved in prostate gland development / methyl-CpG binding / regulation of growth / response to testosterone / prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis / epidermis development / DNA-binding transcription repressor activity, RNA polymerase II-specific / response to wounding / sequence-specific double-stranded DNA binding / angiogenesis ...epithelial cell maturation involved in prostate gland development / methyl-CpG binding / regulation of growth / response to testosterone / prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis / epidermis development / DNA-binding transcription repressor activity, RNA polymerase II-specific / response to wounding / sequence-specific double-stranded DNA binding / angiogenesis / transcription regulator complex / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / chromatin / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / nucleoplasm Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Morgunova, E. / Yin, Y. / Jolma, A. / Popov, A. / Taipale, J. | ||||||
![]() | ![]() Title: Two distinct DNA sequences recognized by transcription factors represent enthalpy and entropy optima. Authors: Morgunova, E. / Yin, Y. / Das, P.K. / Jolma, A. / Zhu, F. / Popov, A. / Xu, Y. / Nilsson, L. / Taipale, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 154.2 KB | Display | ![]() |
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PDB format | ![]() | 116.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 503.3 KB | Display | ![]() |
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Full document | ![]() | 514.1 KB | Display | |
Data in XML | ![]() | 21.9 KB | Display | |
Data in CIF | ![]() | 34.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5ednSC ![]() 6es2C ![]() 6es3C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 5534.570 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: DNA chain | Mass: 5495.624 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: Protein | Mass: 7640.061 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 Details: PEG 5000, potassium chloride, magnesium chloride, PEG 400 PH range: 8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Jul 6, 2015 |
Radiation | Monochromator: Si(111) crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97242 Å / Relative weight: 1 |
Reflection | Resolution: 2.19→46.02 Å / Num. obs: 44173 / % possible obs: 97.5 % / Redundancy: 5.6 % / Rmerge(I) obs: 0.143 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 2.19→2.31 Å / Redundancy: 5.6 % / Rmerge(I) obs: 1.135 / Mean I/σ(I) obs: 1.2 / % possible all: 90.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5edn Resolution: 2.195→45.945 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 37.64 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.195→45.945 Å
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Refine LS restraints |
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LS refinement shell |
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