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- PDB-6p4x: Crystal Structure of the S. cerevisiae glucokinase, Glk1 -

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Basic information

Entry
Database: PDB / ID: 6p4x
TitleCrystal Structure of the S. cerevisiae glucokinase, Glk1
ComponentsGlucokinase-1
KeywordsTRANSFERASE / hexokinase / actin atpase / glycolysis
Function / homology
Function and homology information


Regulation of Glucokinase by Glucokinase Regulatory Protein / glucokinase / Glycolysis / fructokinase activity / carbohydrate phosphorylation / glucokinase activity / mannose metabolic process / glucose 6-phosphate metabolic process / glucose binding / glucose import ...Regulation of Glucokinase by Glucokinase Regulatory Protein / glucokinase / Glycolysis / fructokinase activity / carbohydrate phosphorylation / glucokinase activity / mannose metabolic process / glucose 6-phosphate metabolic process / glucose binding / glucose import / intracellular glucose homeostasis / Neutrophil degranulation / glycolytic process / glucose metabolic process / mitochondrion / ATP binding / plasma membrane / cytosol
Similarity search - Function
Hexokinase; domain 1 / Hexokinase; domain 1 - #20 / Hexokinase / Hexokinase, binding site / Hexokinase, N-terminal / Hexokinase, C-terminal / Hexokinase / Hexokinase / Hexokinase domain signature. / Hexokinase domain profile. ...Hexokinase; domain 1 / Hexokinase; domain 1 - #20 / Hexokinase / Hexokinase, binding site / Hexokinase, N-terminal / Hexokinase, C-terminal / Hexokinase / Hexokinase / Hexokinase domain signature. / Hexokinase domain profile. / ATPase, nucleotide binding domain / ATPase, nucleotide binding domain / Nucleotidyltransferase; domain 5 / 2-Layer Sandwich / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
PHOSPHATE ION / Glucokinase-1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.59 Å
AuthorsStoddard, P.R. / Garner, E.C. / Murray, A.W.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)F31GM116441 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)DP2AI117923 United States
CitationJournal: Science / Year: 2020
Title: Polymerization in the actin ATPase clan regulates hexokinase activity in yeast.
Authors: Patrick R Stoddard / Eric M Lynch / Daniel P Farrell / Annie M Dosey / Frank DiMaio / Tom A Williams / Justin M Kollman / Andrew W Murray / Ethan C Garner /
Abstract: The actin fold is found in cytoskeletal polymers, chaperones, and various metabolic enzymes. Many actin-fold proteins, such as the carbohydrate kinases, do not polymerize. We found that Glk1, a ...The actin fold is found in cytoskeletal polymers, chaperones, and various metabolic enzymes. Many actin-fold proteins, such as the carbohydrate kinases, do not polymerize. We found that Glk1, a glucokinase, forms two-stranded filaments with ultrastructure that is distinct from that of cytoskeletal polymers. In cells, Glk1 polymerized upon sugar addition and depolymerized upon sugar withdrawal. Polymerization inhibits enzymatic activity; the Glk1 monomer-polymer equilibrium sets a maximum rate of glucose phosphorylation regardless of Glk1 concentration. A mutation that eliminated Glk1 polymerization alleviated concentration-dependent enzyme inhibition. Yeast containing nonpolymerizing Glk1 were less fit when growing on sugars and more likely to die when refed glucose. Glk1 polymerization arose independently from other actin-related filaments and may allow yeast to rapidly modulate glucokinase activity as nutrient availability changes.
History
DepositionMay 28, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 11, 2020Provider: repository / Type: Initial release
Revision 1.1Mar 18, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.pdbx_database_id_PubMed ..._citation.journal_volume / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Apr 22, 2020Group: Derived calculations
Category: pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop
Item: _pdbx_struct_assembly.details / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_assembly_prop.value
Revision 1.3Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glucokinase-1
B: Glucokinase-1
C: Glucokinase-1
D: Glucokinase-1
E: Glucokinase-1
F: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)333,91219
Polymers332,6786
Non-polymers1,23513
Water0
1
A: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,7314
Polymers55,4461
Non-polymers2853
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,6363
Polymers55,4461
Non-polymers1902
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,6363
Polymers55,4461
Non-polymers1902
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,6363
Polymers55,4461
Non-polymers1902
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,6363
Polymers55,4461
Non-polymers1902
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)55,6363
Polymers55,4461
Non-polymers1902
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
A: Glucokinase-1
C: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)111,3677
Polymers110,8932
Non-polymers4755
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4070 Å2
ΔGint-32 kcal/mol
Surface area43020 Å2
MethodPISA
8
B: Glucokinase-1
E: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)111,2726
Polymers110,8932
Non-polymers3804
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4230 Å2
ΔGint-29 kcal/mol
Surface area41920 Å2
MethodPISA
9
D: Glucokinase-1
F: Glucokinase-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)111,2726
Polymers110,8932
Non-polymers3804
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4070 Å2
ΔGint-27 kcal/mol
Surface area43400 Å2
MethodPISA
Unit cell
Length a, b, c (Å)177.750, 177.750, 323.947
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number96
Space group name H-MP43212
Space group name HallP4nw2abw
Symmetry operation#1: x,y,z
#2: -y+1/2,x+1/2,z+3/4
#3: y+1/2,-x+1/2,z+1/4
#4: x+1/2,-y+1/2,-z+1/4
#5: -x+1/2,y+1/2,-z+3/4
#6: -x,-y,z+1/2
#7: y,x,-z
#8: -y,-x,-z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
51
61

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ASPASPPROPRO(chain 'A' and (resid 4 through 119 or (resid 120...AA4 - 504 - 50
12LYSLYSVALVAL(chain 'A' and (resid 4 through 119 or (resid 120...AA60 - 49960 - 499
23ASPASPPROPRO(chain 'B' and (resid 4 through 50 or resid 60...BB4 - 504 - 50
24LYSLYSVALVAL(chain 'B' and (resid 4 through 50 or resid 60...BB60 - 49960 - 499
35ASPASPPROPRO(chain 'C' and (resid 4 through 50 or resid 60...CC4 - 504 - 50
36LYSLYSVALVAL(chain 'C' and (resid 4 through 50 or resid 60...CC60 - 49960 - 499
47ASPASPPROPRO(chain 'D' and (resid 4 through 119 or (resid 120...DD4 - 504 - 50
48LYSLYSVALVAL(chain 'D' and (resid 4 through 119 or (resid 120...DD60 - 49960 - 499
59ASPASPPROPRO(chain 'E' and (resid 4 through 119 or (resid 120...EE4 - 504 - 50
510LYSLYSVALVAL(chain 'E' and (resid 4 through 119 or (resid 120...EE60 - 49960 - 499
611ASPASPPROPRO(chain 'F' and (resid 4 through 50 or resid 60...FF4 - 504 - 50
612LYSLYSVALVAL(chain 'F' and (resid 4 through 50 or resid 60...FF60 - 49960 - 499

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Components

#1: Protein
Glucokinase-1 / / Glucose kinase 1 / GLK-1


Mass: 55446.258 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c / Gene: GLK1, HOR3, YCL040W, YCL312, YCL40W / Production host: Escherichia coli (E. coli) / References: UniProt: P17709, glucokinase
#2: Chemical
ChemComp-PO4 / PHOSPHATE ION / Phosphate


Mass: 94.971 Da / Num. of mol.: 13 / Source method: obtained synthetically / Formula: PO4
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.92 Å3/Da / Density % sol: 68.59 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 9.4 / Details: 2.4M Diammonium Phosphate, 0.1M CHES pH 9.4

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9791 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Feb 18, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9791 Å / Relative weight: 1
ReflectionResolution: 3.59→155.83 Å / Num. obs: 61332 / % possible obs: 99.97 % / Redundancy: 2 % / Biso Wilson estimate: 91.07 Å2 / CC1/2: 0.988 / Rmerge(I) obs: 0.1003 / Rpim(I) all: 0.1003 / Rrim(I) all: 0.1418 / Net I/σ(I): 8.28
Reflection shellResolution: 3.59→3.718 Å / Redundancy: 2 % / Rmerge(I) obs: 0.4831 / Mean I/σ(I) obs: 1.56 / Num. unique obs: 6026 / CC1/2: 0.638 / Rpim(I) all: 0.4831 / Rrim(I) all: 0.6832 / % possible all: 99.95

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
Coot1.14_3260model building
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1IG8

1ig8


Resolution: 3.59→155.83 Å / SU ML: 0.5282 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.4634
RfactorNum. reflection% reflection
Rfree0.3002 3105 5.06 %
Rwork0.2668 --
obs0.2685 61322 99.97 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 85.05 Å2
Refinement stepCycle: LAST / Resolution: 3.59→155.83 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms22948 0 5 0 22953
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.004823359
X-RAY DIFFRACTIONf_angle_d1.490331600
X-RAY DIFFRACTIONf_chiral_restr0.07543628
X-RAY DIFFRACTIONf_plane_restr0.00624066
X-RAY DIFFRACTIONf_dihedral_angle_d20.29148733
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.59-3.650.34221490.33952609X-RAY DIFFRACTION99.96
3.65-3.710.38431520.33282563X-RAY DIFFRACTION99.93
3.71-3.770.32681450.33022603X-RAY DIFFRACTION99.96
3.77-3.840.38711280.34312591X-RAY DIFFRACTION99.96
3.84-3.910.35931260.32372622X-RAY DIFFRACTION99.93
3.91-3.990.331320.31292625X-RAY DIFFRACTION100
3.99-4.080.35061450.28792592X-RAY DIFFRACTION99.96
4.08-4.170.34171370.28952607X-RAY DIFFRACTION100
4.17-4.280.30971380.28182624X-RAY DIFFRACTION100
4.28-4.390.27271360.2642630X-RAY DIFFRACTION100
4.39-4.520.29941400.25942619X-RAY DIFFRACTION100
4.52-4.670.28661560.24542632X-RAY DIFFRACTION100
4.67-4.840.29721540.252604X-RAY DIFFRACTION99.96
4.84-5.030.26361660.26112616X-RAY DIFFRACTION100
5.03-5.260.31621420.27022633X-RAY DIFFRACTION100
5.26-5.540.32871350.29342648X-RAY DIFFRACTION99.96
5.54-5.880.30191300.29572674X-RAY DIFFRACTION100
5.88-6.340.33711290.29712681X-RAY DIFFRACTION100
6.34-6.970.32951370.26772691X-RAY DIFFRACTION100
6.97-7.980.28131530.23922687X-RAY DIFFRACTION99.96
7.98-10.060.20791280.18822765X-RAY DIFFRACTION100
10.06-155.830.2591470.22352901X-RAY DIFFRACTION99.67
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.41539467756-0.8316608186310.5924547574860.548081245692-0.1882619469981.03304542759-0.0926001556018-0.1416066796650.1253321153740.5395037260350.278279550982-0.5071490312370.01010442804360.3443445861170.1302592125530.5700780160670.00139753022022-0.06301303844180.4123346577510.03345928464620.957352135841-34.702604476754.9198599216-52.8552130225
21.724217833610.76001459642-0.569465977021.470208355430.03555016583242.82370687699-0.3375230773310.839880869050.0406101827137-0.01848846866080.3413291963350.407704931589-0.1703184014330.3280366348750.1450216023340.5577780369110.130502831186-0.01033629719520.3203483648270.06248239841150.797742020819-56.68502994928.9045508078-17.2268153902
30.674872971869-0.273118062104-0.6072185732150.8877555293020.05913283535441.11390785171-0.595905409811-0.784853107614-0.004188365260791.096178251740.03076942064360.7685180482380.243931940863-0.1388732401020.2060585871910.9530753432280.03925121021860.3864750976350.468827679922-0.05382449959231.32732142199-78.45481392578.351321175854.1332214162
41.26224634680.07730942510120.2679855636661.57994095197-0.1164430145361.93390808142-0.304768730447-0.4511937591-0.6097987844320.684395215060.439248667720.5575763301120.4774650970760.3167662063190.2116184773690.9372343381360.09315873718520.2832410900240.5926281626910.103590313941.06599511606-72.9987523350.119832021517-2.23695892851
51.62767351557-0.152555660555-0.1691219956451.03618311122-0.2148464954220.5865760872790.0445949977783-0.0576358815525-0.6347894909540.4219882690730.0487549064353-0.1255185991960.3748725086250.2288598547150.1958078260960.662013194270.0813980904977-0.04621321918720.3247992020380.05363070876890.834290642774-54.855803250822.924427619-9.28585533144
61.163290096430.485211159595-0.7558023378881.045726701140.5135037430081.29588455725-0.110391650425-0.225904367761-0.1480316822560.424647914623-0.1313435357010.333425051705-0.189281405526-0.2758940727940.1609570834030.7944878258380.1377320385170.04334859197930.3658459274920.004115260427980.782417433262-67.993827519329.192511657-9.05338748491
71.11581406912-0.830692932586-0.3023438390452.43161922540.3355949377411.79533034326-0.226188032621-0.1044961981560.441784176490.49282388801-0.08325112893630.775749592705-0.1253963675760.4245535543210.1329631711080.608924743502-0.0675534514601-0.2624193680290.3885094220860.08527427788050.709811803782-34.219727109249.7763000117-23.7547028156
80.860620377153-0.312539100933-0.5175885339110.5396391285890.5749834591340.656150556306-0.0053667509079-0.1012830655270.530463727222-0.02989902876790.222699541544-0.5432219888410.8402261353181.061498600320.1928732818111.26229756918-0.120925643748-0.1807208366081.512049332590.05302636990181.70127587392-0.67641540981759.8266262505-27.0485620678
90.9731260966930.0965130141414-0.2150136099353.26637507061-0.03118005859531.08118251039-0.07969010852320.04975913364080.1688582554230.344344985211-0.0136656542273-0.558707764633-0.2850892289420.3561474194970.1369802136440.538080605243-0.0493415384531-0.2050885448570.4272682904390.04124263012610.893925350318-25.213522148551.3098721785-28.3772496067
101.25057270106-0.204611913607-0.3554262174481.342493942470.6671861648661.46867415617-0.124527204239-0.01378231368390.4988093375520.2902140510130.0202647862190.180524158871-0.311856493672-0.229541060833-0.1190957410820.6637443772890.09785110971150.01635803327120.5939405004760.006615396664550.886257724374-85.0657055023103.663344082-51.5367273835
111.598229751930.0191122376724-0.7472611852511.711723869570.8279405779411.26012731863-0.0186989322820.05284565536820.2279205356490.2046237923430.02264981372090.144970068231-0.0404958963103-0.397979310038-0.04495048802180.5931110059780.148923563489-0.06162155895090.3665665293050.04714665923030.606696170163-79.788046572490.5532602158-52.2646273394
121.31332210916-0.7000727327050.6603635048020.575502790551-0.2021119886312.55815240062-0.27119890358-0.263732261712-0.3156304517870.3320763962960.2415757392020.2763868575310.383778390262-0.1148711518230.05802895532550.8783255280520.1230912207190.1849772549120.4897357137060.06220011450260.927890935017-91.173769582443.668985903414.4208650249
131.42791788614-0.8947974245830.2452841642371.40212292687-0.009733713194211.99606550222-0.16017626862-0.304249388937-0.3787953510830.2594774507050.1785980663410.06452244979770.376675495579-0.2403664563870.03890152903940.7077680896570.007423940575570.1130154281240.400925119912-0.02237985819410.76879389619-90.008028683244.96961243542.0569919266
141.06385188799-0.348074783223-0.6872103652531.6128051397-0.7441232629231.10018387118-0.1797485777490.332967413256-0.284658439967-0.2995246110490.02122122563860.2076928600530.1407634852790.5730965782590.001220412710190.502077471807-0.00210591945603-0.0780190834170.777920777146-0.2009089753050.739961273511-100.23380077458.8689430678-32.6969209971
150.790498132472-0.891007159603-1.307608674220.986376415321.445322156322.084006811260.3542612038170.749491942873-0.0247159500084-0.671355091242-0.2536095162121.455416821380.183558948799-0.974927786304-0.08997573778521.2862681170.0908340168171-0.3488989307481.998194214930.2418248240221.70916431308-125.48063900971.785410042-58.5174319247
160.8159567744940.146930566034-1.051133740950.997974427262-0.3688405317712.321151162360.01316384088190.4102095529720.122429610974-0.3392818294820.1280476571330.300526797773-0.0687097478291-0.602258302641-0.02925018885050.637598357647-0.0238988591529-0.0865108430030.816393953437-0.1221774447780.80480325865-105.62103925267.687895453-40.4649790841
170.889833269903-0.1032014501720.494881823891.861464499960.2035547239091.10081533062-0.185386215329-0.01412098798760.1719478198870.2285028671610.07306121059360.06340879797620.03045158360640.1227009415650.08138035568060.527019957018-0.04589610254170.02371523826270.2703747645310.0003483732972220.775236431751-41.754718149362.7668133052-61.3308382005
181.372306625840.946215350486-0.8125615050322.596469752760.08920582849171.44410558786-0.2967636347080.3709186236520.141412313582-0.318632281210.315210663817-0.395120169193-0.2508746581560.699032820350.1834850978430.545942564008-0.04555676545730.07502589165640.921655056927-0.01306058317541.16415556374-20.620902823153.1872187798-79.2435009912
191.02843614318-1.59396045664-0.06323740752072.807409731130.3804723105661.344006100910.008963824135340.1821233112110.0287150110074-0.2266470903820.031067126467-0.288227322553-0.1861278171820.1753202442930.06611473287620.461645704424-0.119291058509-0.0496412836930.3152979389050.02514505227970.711343345821-41.197976841867.241963017-61.7203421823
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 433 through 500 )
2X-RAY DIFFRACTION2chain 'B' and (resid 3 through 75 )
3X-RAY DIFFRACTION3chain 'B' and (resid 76 through 109 )
4X-RAY DIFFRACTION4chain 'B' and (resid 110 through 241 )
5X-RAY DIFFRACTION5chain 'B' and (resid 242 through 400 )
6X-RAY DIFFRACTION6chain 'B' and (resid 401 through 500 )
7X-RAY DIFFRACTION7chain 'C' and (resid 1 through 80 )
8X-RAY DIFFRACTION8chain 'C' and (resid 81 through 193 )
9X-RAY DIFFRACTION9chain 'C' and (resid 194 through 500 )
10X-RAY DIFFRACTION10chain 'D' and (resid 4 through 193 )
11X-RAY DIFFRACTION11chain 'D' and (resid 194 through 500 )
12X-RAY DIFFRACTION12chain 'E' and (resid 3 through 187 )
13X-RAY DIFFRACTION13chain 'E' and (resid 188 through 500 )
14X-RAY DIFFRACTION14chain 'F' and (resid 1 through 73 )
15X-RAY DIFFRACTION15chain 'F' and (resid 74 through 202 )
16X-RAY DIFFRACTION16chain 'F' and (resid 203 through 500 )
17X-RAY DIFFRACTION17chain 'A' and (resid 1 through 98 )
18X-RAY DIFFRACTION18chain 'A' and (resid 99 through 227 )
19X-RAY DIFFRACTION19chain 'A' and (resid 228 through 432 )

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