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- PDB-6osr: Crystal structure of Influenza hemagglutinin from strain A/Melbou... -

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Basic information

Entry
Database: PDB / ID: 6osr
TitleCrystal structure of Influenza hemagglutinin from strain A/Melbourne/1/1946(H1N1)
ComponentsHemagglutinin
KeywordsVIRAL PROTEIN / Inflenza hemagglutinin / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID
Function / homology
Function and homology information


viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane
Similarity search - Function
Hemagglutinin (Ha1 Chain); Chain: A; domain 1 / Haemagglutinin, alpha/beta domain, HA1 chain / Haemagglutinin, influenzavirus A / Haemagglutinin, HA1 chain, alpha/beta domain superfamily / Haemagglutinin / Haemagglutinin, influenzavirus A/B / Viral capsid/haemagglutinin protein / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
Biological speciesInfluenza A virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.55 Å
AuthorsSeattle Structural Genomics Center for Infectious Disease (SSGCID)
CitationJournal: Sci Rep / Year: 2023
Title: Structural characterisation of hemagglutinin from seven Influenza A H1N1 strains reveal diversity in the C05 antibody recognition site.
Authors: Ghafoori, S.M. / Petersen, G.F. / Conrady, D.G. / Calhoun, B.M. / Stigliano, M.Z.Z. / Baydo, R.O. / Grice, R. / Abendroth, J. / Lorimer, D.D. / Edwards, T.E. / Forwood, J.K.
History
DepositionMay 2, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 15, 2019Provider: repository / Type: Initial release
Revision 2.0Jul 29, 2020Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_conn_angle / pdbx_validate_close_contact / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.value / _pdbx_validate_close_contact.auth_asym_id_1 / _pdbx_validate_close_contact.auth_asym_id_2 / _pdbx_validate_close_contact.auth_seq_id_1 / _pdbx_validate_close_contact.auth_seq_id_2 / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1May 24, 2023Group: Database references / Derived calculations / Structure summary
Category: chem_comp / citation ...chem_comp / citation / citation_author / database_2 / struct_conn
Item: _chem_comp.pdbx_synonyms / _citation.country ..._chem_comp.pdbx_synonyms / _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag
Revision 2.2Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 2.3Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Hemagglutinin
B: Hemagglutinin
C: Hemagglutinin
D: Hemagglutinin
E: Hemagglutinin
F: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)367,97139
Polymers362,8546
Non-polymers5,11833
Water11,602644
1
A: Hemagglutinin
B: Hemagglutinin
C: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)184,69820
Polymers181,4273
Non-polymers3,27117
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area14730 Å2
ΔGint-22 kcal/mol
Surface area59870 Å2
MethodPISA
2
D: Hemagglutinin
E: Hemagglutinin
F: Hemagglutinin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)183,27419
Polymers181,4273
Non-polymers1,84716
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area14700 Å2
ΔGint-36 kcal/mol
Surface area58250 Å2
MethodPISA
Unit cell
Length a, b, c (Å)73.100, 113.970, 246.380
Angle α, β, γ (deg.)90.000, 91.440, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and ((resid 5 and (name N or name...
21(chain B and ((resid 5 and (name N or name...
31(chain C and ((resid 5 and (name N or name...
41(chain D and ((resid 5 and (name N or name...
51(chain E and (resid 5 through 22 or (resid 23...
61(chain F and ((resid 5 and (name N or name...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and ((resid 5 and (name N or name...A5
121(chain A and ((resid 5 and (name N or name...A4 - 504
131(chain A and ((resid 5 and (name N or name...A4 - 504
141(chain A and ((resid 5 and (name N or name...A4 - 504
151(chain A and ((resid 5 and (name N or name...A4 - 504
211(chain B and ((resid 5 and (name N or name...B5
221(chain B and ((resid 5 and (name N or name...B2 - 501
231(chain B and ((resid 5 and (name N or name...B2 - 501
241(chain B and ((resid 5 and (name N or name...B2 - 501
251(chain B and ((resid 5 and (name N or name...B2 - 501
311(chain C and ((resid 5 and (name N or name...C5
321(chain C and ((resid 5 and (name N or name...C4 - 503
331(chain C and ((resid 5 and (name N or name...C4 - 503
341(chain C and ((resid 5 and (name N or name...C4 - 503
351(chain C and ((resid 5 and (name N or name...C4 - 503
411(chain D and ((resid 5 and (name N or name...D5
421(chain D and ((resid 5 and (name N or name...D4 - 501
431(chain D and ((resid 5 and (name N or name...D4 - 501
441(chain D and ((resid 5 and (name N or name...D4 - 501
451(chain D and ((resid 5 and (name N or name...D4 - 501
511(chain E and (resid 5 through 22 or (resid 23...E5 - 22
521(chain E and (resid 5 through 22 or (resid 23...E23 - 24
531(chain E and (resid 5 through 22 or (resid 23...E5 - 502
541(chain E and (resid 5 through 22 or (resid 23...E5 - 502
551(chain E and (resid 5 through 22 or (resid 23...E5 - 502
561(chain E and (resid 5 through 22 or (resid 23...E5 - 502
611(chain F and ((resid 5 and (name N or name...F5
621(chain F and ((resid 5 and (name N or name...F4 - 503
631(chain F and ((resid 5 and (name N or name...F4 - 503
641(chain F and ((resid 5 and (name N or name...F4 - 503
651(chain F and ((resid 5 and (name N or name...F4 - 503

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Components

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Protein , 1 types, 6 molecules ABCDEF

#1: Protein
Hemagglutinin


Mass: 60475.586 Da / Num. of mol.: 6 / Fragment: UNP residues 18-509
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza A virus (A/Melbourne/1/1946(H1N1))
Strain: A/Melbourne/1/1946(H1N1) / Gene: HA / Plasmid: InvbX.18715.a / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: C7S1Y2

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Sugars , 2 types, 17 molecules

#2: Polysaccharide alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 748.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3/a4-b1_b4-c1_c3-d1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#3: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 16
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 4 types, 660 molecules

#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 11 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Formula: K
#6: Chemical ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Na
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 644 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.83 Å3/Da / Density % sol: 56.5 %
Crystal growTemperature: 287 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 0.1 uL 10.1 mg/mL SEC-purified InvbX.18715.a.KN11.PD38349 in 25 mM Tris, pH 8.5, 150 mM sodium chloride + 0.1 uL mother liquor (0.2 M potassium citrate, 20% PEG3350, 3% 1,5-pentanediol), ...Details: 0.1 uL 10.1 mg/mL SEC-purified InvbX.18715.a.KN11.PD38349 in 25 mM Tris, pH 8.5, 150 mM sodium chloride + 0.1 uL mother liquor (0.2 M potassium citrate, 20% PEG3350, 3% 1,5-pentanediol), cryoprotection: mother liquor + 20% ethylene glycol, Crystal ID 308017b5, data set yxj7-1

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.97872 Å
DetectorType: RAYONIX MX-225 / Detector: CCD / Date: Mar 21, 2019
RadiationMonochromator: diamond(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97872 Å / Relative weight: 1
ReflectionResolution: 2.55→48.736 Å / Num. obs: 131565 / % possible obs: 99.8 % / Redundancy: 5.256 % / Biso Wilson estimate: 46.927 Å2 / CC1/2: 0.992 / Rmerge(I) obs: 0.168 / Rrim(I) all: 0.186 / Χ2: 0.974 / Net I/σ(I): 7.78 / Num. measured all: 691488 / Scaling rejects: 35
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
2.55-2.625.2841.0891.5251334974097150.5481.2199.7
2.62-2.695.3020.9461.7649968944194240.6351.0599.8
2.69-2.775.3050.7972.1448541916891500.7070.88499.8
2.77-2.855.3130.6532.647447895089300.7970.72599.8
2.85-2.945.3280.523.2446340870686980.8520.57799.9
2.94-3.055.3420.4353.8944764838683800.8880.48299.9
3.05-3.165.3320.3334.8942990807080620.9380.36999.9
3.16-3.295.3440.2666.0841556778377760.9590.29699.9
3.29-3.445.2960.217.4639470745674530.9770.233100
3.44-3.615.2790.1689.2137583712471200.9790.18799.9
3.61-3.85.2470.13610.7535904684968430.9870.15199.9
3.8-4.035.2080.11512.1433568644864450.990.128100
4.03-4.315.1730.09614.0131449608160790.9920.107100
4.31-4.665.1510.08515.628905562556110.9920.09599.8
4.66-5.15.1450.08315.7426683519551860.9940.09299.8
5.1-5.75.1590.08315.1324419474047330.9930.09399.9
5.7-6.585.1470.08614.8221397415941570.9920.096100
6.58-8.065.1530.07316.5618304355935520.9930.08299.8
8.06-11.45.0330.05519.2813781274427380.9960.06299.8
11.4-48.7364.6830.05519.527085156115130.9970.06396.9

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassificationNB
PHENIXdev_3409refinement
XDSdata reduction
XSCALEdata scaling
MOLREPphasing
PDB_EXTRACT3.25data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 6MYA

6mya


Resolution: 2.55→48.736 Å / SU ML: 0.35 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 26.75 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2459 1954 1.49 %
Rwork0.1912 129570 -
obs0.1921 131524 99.84 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 210.05 Å2 / Biso mean: 55.7642 Å2 / Biso min: 6.4 Å2
Refinement stepCycle: final / Resolution: 2.55→48.736 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms22122 0 323 646 23091
Biso mean--89.94 42.21 -
Num. residues----2870
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A10647X-RAY DIFFRACTION10.049TORSIONAL
12B10647X-RAY DIFFRACTION10.049TORSIONAL
13C10647X-RAY DIFFRACTION10.049TORSIONAL
14D10647X-RAY DIFFRACTION10.049TORSIONAL
15E10647X-RAY DIFFRACTION10.049TORSIONAL
16F10647X-RAY DIFFRACTION10.049TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
2.5501-2.61380.311340.26592709404
2.6138-2.68450.3081410.259291079248
2.6845-2.76350.33371380.252292259363
2.7635-2.85270.31531180.241792349352
2.8527-2.95460.33221410.235792189359
2.9546-3.07290.31311710.233892329403
3.0729-3.21270.28161300.222892209350
3.2127-3.38210.2711240.195692499373
3.3821-3.59390.22741350.181792489383
3.5939-3.87130.23741460.171993169462
3.8713-4.26070.1771230.150792419364
4.2607-4.87670.18731580.140592859443
4.8767-6.14220.19521300.175593239453
6.1422-48.74540.25481650.199494029567
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.3166-0.7231-1.40760.70950.82723.8745-0.1171-0.4649-0.12040.42830.18880.01930.27030.1746-0.01910.47560.0357-0.05410.33560.060.349421.5238-15.193653.8828
21.4401-0.0269-0.78531.89670.06342.97780.05110.1525-0.0929-0.08470.0099-0.0286-0.03990.0036-0.06350.22480.0068-0.09170.146-0.01350.297226.4793-15.39320.219
31.1536-0.1809-0.51890.60540.1111.4449-0.1805-0.7018-0.25030.39880.13590.19870.43940.13080.0680.76760.09690.03640.76920.13590.35427.0272-9.298480.205
41.27130.352-1.59150.7321-1.23057.92010.0785-0.06060.10550.2436-0.00340.2496-0.1349-0.7969-0.06660.3342-0.00250.00950.3256-0.01150.4437-16.359-1.451.8993
53.01370.3515-1.21681.2642-0.63193.2725-0.15130.5075-0.1299-0.1740.17160.0810.0906-0.237-0.02250.2450.0022-0.11570.3035-0.02710.3697-0.88714.830915.9745
60.8806-0.0245-0.74150.68520.09193.3001-0.0245-0.4291-0.06610.369-0.02570.2053-0.1207-0.4570.03540.50230.04560.04120.62620.03210.3504-13.35151.191574.0388
71.39930.3946-1.50840.4992-0.92432.24040.2963-0.31240.30860.4197-0.0450.1331-0.5082-0.0401-0.25560.4915-0.04880.02430.3672-0.12710.379417.387722.948152.5668
81.2963-0.1166-0.80131.17410.34533.10810.08960.01670.1378-0.0161-0.06350.0307-0.097-0.1109-0.02750.2467-0.0208-0.06580.1625-0.02790.335629.807618.900224.7881
91.04830.2044-0.73660.35120.07793.44040.1997-0.62540.21670.50940.01330.082-0.27790.2512-0.1910.74090.00860.07640.6442-0.10650.35865.112814.141975.828
100.63520.14520.60580.11670.11070.57050.1546-0.97150.3750.99440.16520.2235-0.91370.3983-0.13951.631-0.11080.26921.7258-0.22910.5311-1.272115.3315113.148
110.8568-0.0721-0.20290.5218-0.14043.5498-0.0543-0.23910.04240.03290.05810.1610.2992-0.401-0.01510.2528-0.0384-0.03090.2996-0.00770.357422.2124-51.143247.0369
123.12050.4972-3.61053.1115-1.35815.08210.04250.371-0.1677-0.0590.04190.18140.0056-0.474-0.08750.2934-0.0154-0.16560.2288-0.02650.304630.3644-46.7819.29
132.204-1.0088-0.63094.16260.9782.6618-0.11390.13230.0057-0.21620.1805-0.19240.04510.0173-0.08910.2461-0.0327-0.04510.15290.00370.238140.3717-47.753416.5799
145.54234.1591-3.61194.4696-4.17475.2556-0.104-0.59520.1132-0.0221-0.1316-0.15720.0115-0.02430.2060.21640.0832-0.01530.2551-0.08460.32322.126-45.023441.2662
150.76040.0639-0.98210.6802-0.89656.55250.0634-0.28560.11530.4893-0.13740.16240.0455-0.29530.03770.4472-0.06650.03710.6507-0.050.290821.2994-54.360767.8714
161.5276-0.9186-0.18423.2121.9091.2385-0.0319-0.1562-0.17130.95030.20350.614-0.6156-0.355-0.15431.0911-0.11590.26091.5214-0.06630.54377.2001-51.121995.8336
171.614-0.4669-2.14360.59740.83015.39340.1127-0.49940.21440.35550.12930.0543-0.215-0.3242-0.22660.4950.03630.07010.6568-0.05510.307728.1412-52.24372.5014
180.647-0.7011-0.49190.7580.52830.36970.0764-0.92880.47360.49320.173-0.02170.5514-0.362-0.19341.39360.15070.52122.0172-0.24230.61689.7708-46.167108.7773
190.48910.1217-1.66040.1854-0.21465.54920.3962-0.70340.49970.4383-0.06830.0288-0.42010.1023-0.27830.7449-0.04680.00120.7529-0.23990.459144.3468-35.741580.9163
202.0643-0.12540.02791.274-0.10251.8053-0.01080.12820.1389-0.07190.0048-0.0722-0.20290.07250.0020.2241-0.0662-0.03180.2754-0.05040.321966.5192-41.522931.2915
210.80210.1377-0.15250.4379-0.34722.65590.1755-0.70380.21650.5512-0.0227-0.067-0.32620.0178-0.12190.7717-0.0201-0.00980.8508-0.1710.323742.8247-43.613484.7473
220.6908-0.2739-0.2920.60390.96342.6274-0.0632-0.4303-0.21390.51940.2028-0.09320.82220.1587-0.09530.66890.1054-0.09030.48390.07490.406649.5109-75.710865.2108
231.7440.6364-0.12112.04840.15872.399-0.11460.1426-0.2032-0.10740.205-0.37050.00350.2821-0.080.2238-0.0021-0.05370.2125-0.07890.356754.9373-73.767625.2398
240.83990.0364-1.16880.28820.70744.6181-0.1948-0.6345-0.26340.61820.17190.03110.47570.06580.05660.81330.0816-0.04360.76070.0980.366643.4211-72.29880.05
252.9483.2204-3.90653.4611-4.36018.36530.21020.32120.29460.61910.48410.23440.1545-1.4315-0.63280.67950.0758-0.04470.5650.11660.387737.9046-67.625768.424
260.98950.2083-1.5080.8783-0.39314.98130.1659-0.6013-0.02150.79780.0014-0.05090.0778-0.0447-0.13820.8455-0.0747-0.01660.75430.04530.21238.3721-59.74981.0011
270.13370.01110.10830.1609-0.14060.2217-0.4948-0.8743-0.43510.9610.25010.42710.5485-0.34190.13521.9880.10210.45251.70360.40540.740824.0401-71.012113.987
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 117 )A4 - 117
2X-RAY DIFFRACTION2chain 'A' and (resid 118 through 269 )A118 - 269
3X-RAY DIFFRACTION3chain 'A' and (resid 270 through 492 )A270 - 492
4X-RAY DIFFRACTION4chain 'B' and (resid 2 through 83 )B2 - 83
5X-RAY DIFFRACTION5chain 'B' and (resid 84 through 280 )B84 - 280
6X-RAY DIFFRACTION6chain 'B' and (resid 281 through 486 )B281 - 486
7X-RAY DIFFRACTION7chain 'C' and (resid 4 through 117 )C4 - 117
8X-RAY DIFFRACTION8chain 'C' and (resid 118 through 284 )C118 - 284
9X-RAY DIFFRACTION9chain 'C' and (resid 285 through 458 )C285 - 458
10X-RAY DIFFRACTION10chain 'C' and (resid 459 through 492 )C459 - 492
11X-RAY DIFFRACTION11chain 'D' and (resid 4 through 117 )D4 - 117
12X-RAY DIFFRACTION12chain 'D' and (resid 118 through 162 )D118 - 162
13X-RAY DIFFRACTION13chain 'D' and (resid 163 through 254 )D163 - 254
14X-RAY DIFFRACTION14chain 'D' and (resid 255 through 284 )D255 - 284
15X-RAY DIFFRACTION15chain 'D' and (resid 285 through 337 )D285 - 337
16X-RAY DIFFRACTION16chain 'D' and (resid 338 through 366 )D338 - 366
17X-RAY DIFFRACTION17chain 'D' and (resid 367 through 461 )D367 - 461
18X-RAY DIFFRACTION18chain 'D' and (resid 462 through 491 )D462 - 491
19X-RAY DIFFRACTION19chain 'E' and (resid 5 through 48 )E5 - 48
20X-RAY DIFFRACTION20chain 'E' and (resid 49 through 269 )E49 - 269
21X-RAY DIFFRACTION21chain 'E' and (resid 270 through 492 )E270 - 492
22X-RAY DIFFRACTION22chain 'F' and (resid 4 through 83 )F4 - 83
23X-RAY DIFFRACTION23chain 'F' and (resid 84 through 269 )F84 - 269
24X-RAY DIFFRACTION24chain 'F' and (resid 270 through 366 )F270 - 366
25X-RAY DIFFRACTION25chain 'F' and (resid 367 through 403 )F367 - 403
26X-RAY DIFFRACTION26chain 'F' and (resid 404 through 461 )F404 - 461
27X-RAY DIFFRACTION27chain 'F' and (resid 462 through 492 )F462 - 492

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