詳細 | タイプ | Solution-ID | 内容 | Label | 溶媒系 |
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solution1 | 1 mM U-15N; U-13-; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O | 15N-, 13C-, and 2H-isotopically enriched | 100% D2Osolution2 | 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O | 15N-, and 2H-isotopically enriched | 100% D2Osolution3 | 1 mM U-15N; U-13-; U-2H I13C MT-DOTA-Lu/Tm, 100% D2O | I13C MT-DOTA-Lu/Tm | 100% D2O | | | | | |
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試料 | 濃度 (mg/ml) | 構成要素 | Isotopic labeling | Solution-ID |
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1 mM15N-, 13C-, and 2H-isotopically enriched | U-15N; U-13-; U-2H | 1 | 1 mM15N-, and 2H-isotopically enriched | U-15N; U-2H | 2 | 1 mMI13C MT-DOTA-Lu/Tm | U-15N; U-13-; U-2H | 3 | | | |
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試料状態 | Conditions-ID | イオン強度 | Label | pH | 圧 (kPa) | 温度 (K) | 詳細 |
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1 | 20 mM2H-isotopically enriched sample | 6.3 | 1 bar293 K | 2 | 25 mMpH 7.8 for HX rates measurement7.8 | 1 bar293 K1-mM sample of perdeuterated, 15N-enriched MT sample in 95% H2O, 5% D2O, 25 mM sodium phosphate buffer, pH 7.8 | 3 | 25 mMpf1 sample6.3 | 1 bar293 K13 mg/mL Pf1 in 25 mM sodium phosphate buffer, pH 6.3, 2 mM EDTA5 | 100 mMpf1 sample (high salt)6.3 | 1 bar293 K13 mg/mL Pf1 in 25 mM sodium phosphate buffer, pH 6.3, 2 mM EDTA, an additional 100 mM NaCl4 | 25 mMTm/Lu lanthanide aligned sample6.3 | 1 bar293 K | | | | | | | | | | | | | | | | | | | | | |
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