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- PDB-6o6n: Structure of the regulator FasR from Mycobacterium tuberculosis i... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6o6n | ||||||
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Title | Structure of the regulator FasR from Mycobacterium tuberculosis in complex with C20-CoA | ||||||
![]() | TetR family transcriptional regulator | ||||||
![]() | TRANSCRIPTION / TetR-like transcription factor / fatty acid biosynthesis regulation | ||||||
Function / homology | ![]() regulation of gene expression / transcription cis-regulatory region binding / DNA-binding transcription factor activity / regulation of DNA-templated transcription / DNA binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Larrieux, N. / Trajtenberg, F. / Lara, J. / Gramajo, H. / Buschiazzo, A. | ||||||
![]() | ![]() Title: Mycobacterium tuberculosis FasR senses long fatty acyl-CoA through a tunnel and a hydrophobic transmission spine. Authors: Lara, J. / Diacovich, L. / Trajtenberg, F. / Larrieux, N. / Malchiodi, E.L. / Fernandez, M.M. / Gago, G. / Gramajo, H. / Buschiazzo, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 98 KB | Display | ![]() |
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PDB format | ![]() | 72.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 561.9 KB | Display | ![]() |
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Full document | ![]() | 563.2 KB | Display | |
Data in XML | ![]() | 11.8 KB | Display | |
Data in CIF | ![]() | 17 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6o6oC ![]() 6o6pC C: citing same article ( |
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Similar structure data | |
Experimental dataset #1 | Data reference: ![]() Details: X-Ray Diffraction data from FasR transcription factor from Mycobacterium tuberculosis in complex with C20-CoA, source of 6O6N structure Metadata reference: 10.15785/SBGRID/647 |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 21592.369 Da / Num. of mol.: 1 / Fragment: delta-33 construct (UNP residues 35-225) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: kstR2_2, kstR2_1, kstR2_3, kstR2_4, kstR2_5, kstR2_6, kstR2_7, DSI35_30495, ERS007661_03222, ERS007663_03464, ERS007665_00400, ERS007720_01056, ERS007741_00500, ERS023446_00266, ERS027646_ ...Gene: kstR2_2, kstR2_1, kstR2_3, kstR2_4, kstR2_5, kstR2_6, kstR2_7, DSI35_30495, ERS007661_03222, ERS007663_03464, ERS007665_00400, ERS007720_01056, ERS007741_00500, ERS023446_00266, ERS027646_02562, ERS027651_00856, ERS027652_02035, ERS027653_03199, ERS027656_00595, ERS027666_01457, ERS124361_00903, SAMEA2682864_03848, SAMEA2683035_03897 Plasmid: pET28a / Production host: ![]() ![]() | ||
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#2: Chemical | ChemComp-5F9 / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.95 Å3/Da / Density % sol: 58.3 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.7 Details: 2.2 M sodium chloride, 0.1 M sodium acetate trihydrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 14, 2016 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97857 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.7→20.2 Å / Num. obs: 28969 / % possible obs: 99.8 % / Redundancy: 14.181 % / Biso Wilson estimate: 22.84 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.065 / Rrim(I) all: 0.067 / Χ2: 1.132 / Net I/σ(I): 22.58 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 1.7→20.2 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.943 / SU R Cruickshank DPI: 0.092 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.1 / SU Rfree Blow DPI: 0.097 / SU Rfree Cruickshank DPI: 0.092
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Displacement parameters | Biso max: 108.12 Å2 / Biso mean: 25.3 Å2 / Biso min: 3.94 Å2
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Refine analyze | Luzzati coordinate error obs: 0.21 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.7→20.2 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.71 Å / Rfactor Rfree error: 0 / Total num. of bins used: 50
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Refinement TLS params. | Method: refined / Origin x: 20.09 Å / Origin y: -29.0942 Å / Origin z: 2.817 Å
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Refinement TLS group | Selection details: { A|* } |