Mass: 18893.021 Da / Num. of mol.: 1 / Fragment: 562-725 C-terminal fragment Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: EEF2K / Plasmid: pET-26b / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O00418, elongation factor 2 kinase
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
3D 1H-15N NOESY
1
2
4
isotropic
1
4D HC-HSQC-NOESY-HN-HSQC
1
3
2
isotropic
3
3D 3D NNH (15N-HSQC-NOESY-15N-HSQC
1
4
4
isotropic
2
3D 1H-13C NOESY aliphatic
1
5
4
isotropic
2
3D H-13C NOESY aromatic
1
6
2
isotropic
4
3D HNCO
1
7
2
isotropic
4
3D HNCA
1
8
2
isotropic
5
3DHN(CO)CA
1
9
2
isotropic
4
3D HN(CA)CB
1
10
2
isotropic
4
3DCBCA(CO)NH
1
11
2
isotropic
4
3DHN(CA)CO
1
12
2
isotropic
5
3DHcc(co)NH
1
13
2
anisotropic
5
3DhCc(co)NH
1
14
2
isotropic
5
3D (H)CCH-TOCSY
1
15
2
isotropic
5
3D (H)CCH-TOCSY
1
16
3
anisotropic
4
2D 15N HSQC-IPAP
1
17
3
anisotropic
4
3D IPAP-J-HNCO
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Sample preparation
Details
Type
Solution-ID
Contents
Label
Solvent system
solution
1
200 uM [U-99% 13C; U-99% 15N] eEF2K (562-725), 20 mM sodium phosphate, 100 mM sodium chloride, 4 mM DTT, 0.2 mM EDTA, 2 mM AEBSF protease inhibitor, 95% H2O/5% D2O
15N13C
95% H2O/5% D2O
solution
4
360 uM [U-99% 13C; U-99% 15N] eEF2K (562-725), 20 mM sodium phosphate, 100 mM sodium chloride, 4 mM DTT, 0.2 mM EDTA, 2 mM AEBSF protease inhibitor, 95% H2O/5% D2O
15N13C_3
95% H2O/5% D2O
solution
2
400 uM [U-99% 13C; U-99% 15N] eEF2K (562-725), 20 mM sodium phosphate, 100 mM sodium chloride, 4 mM DTT, 0.2 mM EDTA, 2 mM AEBSF protease inhibitor, 95% H2O/5% D2O
15N13C_2
95% H2O/5% D2O
filamentousvirus
3
340 uM [U-99% 13C; U-99% 15N] eEF2K (562-725), 14 mg/mL Pf1 phage, 14 mM sodium phosphate, 100 mM sodium chloride, 4 mM DTT, 0.2 mM EDTA, 2 mM AEBSF protease inhibitor, 95% H2O/5% D2O