Details | Type | Solution-ID | Contents | Label | Solvent system |
---|
solution1 | 1.0 mM [A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] RNA, 100% D2O | SL-1 | 100% D2Osolution2 | 1.0 mM RNA, 100% D2OSL-2 | 100% D2Osolution3 | 1.0 mM RNA, 90% H2O/10% D2OSL-3 | 90% H2O/10% D2Osolution4 | 1.0 mM RNA, 100% D2OTL-1 | 100% D2Osolution5 | 1.0 mM RNA, 90% H2O/10% D2OTL-2 | 90% H2O/10% D2Osolution6 | 1.0 mM [A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] RNA, 100% D2O | TL-3 | 100% D2Osolution7 | 1.0 mM [A,C,G,U-98% 13C, A,C,G,U-98% 15N] RNA, 100% D2O | TL-4 | 100% D2Osolution8 | 1.0 mM [A,C,G,U-98% 13C, A,C,G,U-98% 15N] RNA, 90% H2O/10% D2O | TL-5 | 90% H2O/10% D2O | | | | | | | | | | | | | | | | | | | |
|
---|
Sample | Conc. (mg/ml) | Component | Isotopic labeling | Solution-ID |
---|
1.0 mMRNA[A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] | 1 | 1.0 mMRNAnatural abundance2 | 1.0 mMRNAnatural abundance3 | 1.0 mMRNAnatural abundance4 | 1.0 mMRNAnatural abundance5 | 1.0 mMRNA[A,G-98% D3', 4', 5', 5"; C,U-98% D3', 4', 5', 5", 5] | 6 | 1.0 mMRNA[A,C,G,U-98% 13C, A,C,G,U-98% 15N] | 7 | 1.0 mMRNA[A,C,G,U-98% 13C, A,C,G,U-98% 15N] | 8 | | | | | | | | | | | | | | | | | | | | |
|
---|
Sample conditions | Conditions-ID | Ionic strength | Label | pH | Pressure (kPa) | Temperature (K) |
---|
1 | 20mM Sodium Phosphate Not definedconditions-1 | 6.1 | 1 atm298 K2 | 20mM Sodium Phosphate Not definedconditions-2 | 6.1 | 1 atm278 K | | | | | |
|
---|