- PDB-6n7r: Saccharomyces cerevisiae spliceosomal E complex (ACT1) -
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Basic information
Entry
Database: PDB / ID: 6n7r
Title
Saccharomyces cerevisiae spliceosomal E complex (ACT1)
Components
(Small nuclear ribonucleoprotein ...SnRNP) x 6
(U1 small nuclear ribonucleoprotein ...) x 5
56 kDa U1 small nuclear ribonucleoprotein component
ACT1 pre-mRNA
Pre-mRNA-processing factor 39
Protein LUC7
Protein NAM8
Small nuclear ribonucleoprotein-associated protein B
U1 snRNAU1 spliceosomal RNA
Keywords
RNA BINDING PROTEIN / pre-mRNA splicing / spliceosome / E complex
Function / homology
Function and homology information
positive regulation of RNA binding / splicing factor binding / snRNA binding / mRNA splice site selection / pICln-Sm protein complex / U4 snRNP / positive regulation of mRNA splicing, via spliceosome / small nuclear ribonucleoprotein complex / spliceosomal tri-snRNP complex / SMN-Sm protein complex ...positive regulation of RNA binding / splicing factor binding / snRNA binding / mRNA splice site selection / pICln-Sm protein complex / U4 snRNP / positive regulation of mRNA splicing, via spliceosome / small nuclear ribonucleoprotein complex / spliceosomal tri-snRNP complex / SMN-Sm protein complex / commitment complex / U2 snRNP / U12-type spliceosomal complex / pre-mRNA 5'-splice site binding / U2-type prespliceosome / spliceosomal snRNP assembly / sno(s)RNA-containing ribonucleoprotein complex / poly(U) RNA binding / U1 snRNP / precatalytic spliceosome / mRNA 5'-splice site recognition / U5 snRNP / U4/U6 x U5 tri-snRNP complex / U1 snRNA binding / catalytic step 2 spliceosome / mRNA splicing, via spliceosome / ribonucleoprotein complex / mRNA binding / mitochondrion / RNA binding / zinc ion binding / nucleus / cytosol / cytoplasm
Nucleotide-binding alpha-beta plait domain superfamily / Small nuclear ribonucleoprotein Sm D2 / Matrin/U1-C-like, C2H2-type zinc finger / HAT (Half-A-TPR) repeat / PWI domain / LSM domain, eukaryotic/archaea-type / Matrin/U1-C, C2H2-type zinc finger / RNA recognition motif domain / Tetratricopeptide-like helical domain superfamily / U1-C, C2H2-type zinc finger ...Nucleotide-binding alpha-beta plait domain superfamily / Small nuclear ribonucleoprotein Sm D2 / Matrin/U1-C-like, C2H2-type zinc finger / HAT (Half-A-TPR) repeat / PWI domain / LSM domain, eukaryotic/archaea-type / Matrin/U1-C, C2H2-type zinc finger / RNA recognition motif domain / Tetratricopeptide-like helical domain superfamily / U1-C, C2H2-type zinc finger / Sm-like protein Lsm6/SmF / U1 small nuclear ribonucleoprotein C / U1 small nuclear ribonucleoprotein of 70kDa N-terminal / Small nuclear ribonucleoprotein E / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / LSM domain superfamily / Luc7-related / Small nuclear ribonucleoprotein G / Zinc finger C2H2 superfamily / LSM domain / LUC7 N_terminus / Snu56-like U1 small nuclear ribonucleoprotein component / U1 zinc finger / U1 small nuclear ribonucleoprotein of 70kDa MW N terminal / RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) / Snu56-like U1 small nuclear ribonucleoprotein component / in:ipr040252: / RNA-binding domain superfamily / snRNP70, RNA recognition motif / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein Sm D3 / SH3 type barrels. - #100 / SH3 type barrels. / Roll / Mainly Beta
Pre-mRNA-processing factor 39 / Small nuclear ribonucleoprotein E / gb:1039023756: / Protein LUC7 / Small nuclear ribonucleoprotein-associated protein B / Small nuclear ribonucleoprotein Sm D2 / U1 small nuclear ribonucleoprotein C / 56 kDa U1 small nuclear ribonucleoprotein component / U1 small nuclear ribonucleoprotein component PRP42 / Small nuclear ribonucleoprotein Sm D1 ...Pre-mRNA-processing factor 39 / Small nuclear ribonucleoprotein E / gb:1039023756: / Protein LUC7 / Small nuclear ribonucleoprotein-associated protein B / Small nuclear ribonucleoprotein Sm D2 / U1 small nuclear ribonucleoprotein C / 56 kDa U1 small nuclear ribonucleoprotein component / U1 small nuclear ribonucleoprotein component PRP42 / Small nuclear ribonucleoprotein Sm D1 / U1 small nuclear ribonucleoprotein 70 kDa homolog / Protein NAM8 / Small nuclear ribonucleoprotein F / U1 small nuclear ribonucleoprotein A / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein G / U1 small nuclear ribonucleoprotein component SNU71
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
GM126157
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
GM114178
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
GM071940
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
AI094386
United States
Citation
Journal: Nature / Year: 2019 Title: A unified mechanism for intron and exon definition and back-splicing. Authors: Xueni Li / Shiheng Liu / Lingdi Zhang / Aaron Issaian / Ryan C Hill / Sara Espinosa / Shasha Shi / Yanxiang Cui / Kalli Kappel / Rhiju Das / Kirk C Hansen / Z Hong Zhou / Rui Zhao / Abstract: The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E ...The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E complex assembled on introns, providing a view of the earliest event in the splicing cycle that commits pre-mRNAs to splicing. The E complex architecture suggests that the same spliceosome can assemble across an exon, and that it either remodels to span an intron for canonical linear splicing (typically on short exons) or catalyses back-splicing to generate circular RNA (on long exons). The model is supported by our experiments, which show that an E complex assembled on the middle exon of yeast EFM5 or HMRA1 can be chased into circular RNA when the exon is sufficiently long. This simple model unifies intron definition, exon definition, and back-splicing through the same spliceosome in all eukaryotes and should inspire experiments in many other systems to understand the mechanism and regulation of these processes.
A: U1 small nuclear ribonucleoprotein 70 kDa homolog B: U1 small nuclear ribonucleoprotein C C: U1 small nuclear ribonucleoprotein A D: U1 small nuclear ribonucleoprotein component PRP42 E: Pre-mRNA-processing factor 39 F: Protein NAM8 G: 56 kDa U1 small nuclear ribonucleoprotein component H: U1 small nuclear ribonucleoprotein component SNU71,U1 small nuclear ribonucleoprotein component SNU71,Snu71 I: Protein LUC7 K: Small nuclear ribonucleoprotein-associated protein B L: Small nuclear ribonucleoprotein Sm D1 M: Small nuclear ribonucleoprotein Sm D2 N: Small nuclear ribonucleoprotein Sm D3 O: Small nuclear ribonucleoprotein E P: Small nuclear ribonucleoprotein F Q: Small nuclear ribonucleoprotein G R: U1 snRNA r: ACT1 pre-mRNA hetero molecules
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