- PDB-6mc6: Crystal structure of PprA filament from Deinococcus radiodurans -
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Basic information
Entry
Database: PDB / ID: 6mc6
Title
Crystal structure of PprA filament from Deinococcus radiodurans
Components
DNA repair protein PprA
Keywords
DNA BINDING PROTEIN / DNA damage repair / Radiation induced / Genome segregation / Filment formation
Function / homology
cellular response to desiccation / positive regulation of DNA ligation / cellular response to gamma radiation / double-stranded DNA binding / damaged DNA binding / DNA repair / DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
A: DNA repair protein PprA B: DNA repair protein PprA
defined by author
Evidence: gel filtration, Purified PprA elutes from a gel filtration column at a broad distribution of retention volumes. The retention volume corresponds to an oligomeric size ranging from a ...Evidence: gel filtration, Purified PprA elutes from a gel filtration column at a broad distribution of retention volumes. The retention volume corresponds to an oligomeric size ranging from a tetramer to a 50-mer and larger., scanning transmission electron microscopy, In vitro, PprA exists as a long filament of varying length, as observed by TEM. This behavior is independent of Deinococcus species of origin., microscopy, In vivo, PprA forms a thin strand spanning the septum of dividing D.radiodurans cells. This was visualized by fluorescence microscopy., The repeating filament assembly present in this crystal has been observed in numerous other crystal forms, despite different symmetries and crystal packing., Residues that are required for DNA binding all map to two distinct faces of this assembly.
Mass: 18.015 Da / Num. of mol.: 44 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.5 Å3/Da / Density % sol: 50.8 % / Description: long rectangular prism
Crystal grow
Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 8 Details: Protein at 4.5 mg/mL in 150 mM KCl, 20 mM Tris, pH 7.5 was mixed in 1:1 volume ratio with Wizard Classic 1 (Rigaku) #46 - a solution of 200 mM Lithium sulfate, 10% (w/v) PEG 8000 and 100 mM ...Details: Protein at 4.5 mg/mL in 150 mM KCl, 20 mM Tris, pH 7.5 was mixed in 1:1 volume ratio with Wizard Classic 1 (Rigaku) #46 - a solution of 200 mM Lithium sulfate, 10% (w/v) PEG 8000 and 100 mM Imidazole/ HCl at pH 8.0. The drop was suspended over a 1.5M Ammonium sulfate dehydrating solution and incubated for about 5 weeks. Temp details: Temperature-controlled incubation
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Data collection
Diffraction
Mean temperature: 100 K / Ambient temp details: Nitrogen gas cryo-stream
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