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- PDB-6m7k: Structure of mouse RECON (AKR1C13) in complex with cyclic AMP-AMP... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6m7k | ||||||
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Title | Structure of mouse RECON (AKR1C13) in complex with cyclic AMP-AMP-GMP (cAAG) | ||||||
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![]() | OXIDOREDUCTASE / RECON / innate immunity / AKR1C13 / cyclic AMP-AMP-GMP | ||||||
Function / homology | ![]() : / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / xenobiotic metabolic process Similarity search - Function | ||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Eaglesham, J.B. / Whiteley, A.T. / de Oliveira Mann, C.C. / Morehouse, B.R. / Nieminen, E.A. / King, D.S. / Lee, A.S.Y. / Mekalanos, J.J. / Kranzusch, P.J. | ||||||
![]() | ![]() Title: Bacterial cGAS-like enzymes synthesize diverse nucleotide signals. Authors: Whiteley, A.T. / Eaglesham, J.B. / de Oliveira Mann, C.C. / Morehouse, B.R. / Lowey, B. / Nieminen, E.A. / Danilchanka, O. / King, D.S. / Lee, A.S.Y. / Mekalanos, J.J. / Kranzusch, P.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 229.6 KB | Display | ![]() |
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PDB format | ![]() | 182.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.6 KB | Display | ![]() |
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Full document | ![]() | 469.8 KB | Display | |
Data in XML | ![]() | 20.6 KB | Display | |
Data in CIF | ![]() | 32.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6e0kC ![]() 6e0lC ![]() 6e0mC ![]() 6e0nC ![]() 6e0oC ![]() 5uxfS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 36758.188 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q8VC28, Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor | ||
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#2: RNA chain | Mass: 958.660 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) | ||
#3: Chemical | ChemComp-EDO / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.98 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop Details: 1.0 M lithium chloride, 0.1 M sodium acetate, 30% PEG6000 |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Aug 16, 2018 |
Radiation | Monochromator: Cryo-cooled double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 1.1→46.02 Å / Num. obs: 130194 / % possible obs: 99.7 % / Redundancy: 9.1 % / CC1/2: 0.999 / Rpim(I) all: 0.029 / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.1→1.12 Å / Redundancy: 8.2 % / Mean I/σ(I) obs: 2.6 / Num. unique obs: 6242 / CC1/2: 0.813 / Rsym value: 0.298 / % possible all: 97.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 5UXF Resolution: 1.1→46.02 Å / SU ML: 0.08 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 14.48
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.1→46.02 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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