+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6lx3 | ||||||
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タイトル | Cryo-EM structure of human secretory immunoglobulin A | ||||||
要素 |
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キーワード | IMMUNE SYSTEM / immunoglobulin / dimer / transcytosis / secreted | ||||||
機能・相同性 | 機能・相同性情報 polymeric immunoglobulin receptor activity / immunoglobulin transcytosis in epithelial cells mediated by polymeric immunoglobulin receptor / kappa-type opioid receptor binding / dimeric IgA immunoglobulin complex / regulation of CD4-positive, alpha-beta T cell proliferation / regulation of T cell homeostatic proliferation / polymeric immunoglobulin binding / interleukin-2 receptor binding / secretory dimeric IgA immunoglobulin complex / pentameric IgM immunoglobulin complex ...polymeric immunoglobulin receptor activity / immunoglobulin transcytosis in epithelial cells mediated by polymeric immunoglobulin receptor / kappa-type opioid receptor binding / dimeric IgA immunoglobulin complex / regulation of CD4-positive, alpha-beta T cell proliferation / regulation of T cell homeostatic proliferation / polymeric immunoglobulin binding / interleukin-2 receptor binding / secretory dimeric IgA immunoglobulin complex / pentameric IgM immunoglobulin complex / monomeric IgA immunoglobulin complex / secretory IgA immunoglobulin complex / response to tacrolimus / positive regulation of plasma cell differentiation / glycosphingolipid binding / negative regulation of lymphocyte proliferation / Fc receptor signaling pathway / negative regulation of T-helper 17 cell differentiation / IgA binding / positive regulation of tissue remodeling / glomerular filtration / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / detection of chemical stimulus involved in sensory perception of bitter taste / IgA immunoglobulin complex / leukocyte activation involved in immune response / positive regulation of isotype switching to IgG isotypes / interleukin-2-mediated signaling pathway / activated T cell proliferation / natural killer cell activation / kinase activator activity / positive regulation of regulatory T cell differentiation / : / negative regulation of B cell apoptotic process / IgG immunoglobulin complex / Interleukin-2 signaling / positive regulation of immunoglobulin production / azurophil granule membrane / positive regulation of dendritic spine development / receptor clustering / positive regulation of activated T cell proliferation / positive regulation of respiratory burst / positive regulation of interleukin-17 production / humoral immune response / T cell differentiation / Interleukin receptor SHC signaling / Scavenging of heme from plasma / immunoglobulin complex, circulating / immunoglobulin receptor binding / positive regulation of B cell proliferation / extrinsic apoptotic signaling pathway in absence of ligand / positive regulation of tyrosine phosphorylation of STAT protein / complement activation, classical pathway / negative regulation of protein phosphorylation / cytokine activity / antigen binding / Cell surface interactions at the vascular wall / B cell receptor signaling pathway / growth factor activity / epidermal growth factor receptor signaling pathway / negative regulation of inflammatory response / positive regulation of inflammatory response / positive regulation of type II interferon production / cell-cell signaling / protein-macromolecule adaptor activity / antibacterial humoral response / positive regulation of cytosolic calcium ion concentration / RAF/MAP kinase cascade / positive regulation of cell growth / carbohydrate binding / protein-containing complex assembly / response to ethanol / adaptive immune response / transcription by RNA polymerase II / blood microparticle / receptor complex / cell adhesion / immune response / innate immune response / positive regulation of cell population proliferation / Neutrophil degranulation / negative regulation of apoptotic process / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular exosome / extracellular region / plasma membrane 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.15 Å | ||||||
データ登録者 | Wang, Y. / Wang, G. / Li, Y. / Xiao, J. | ||||||
資金援助 | 中国, 1件
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引用 | ジャーナル: Cell Res / 年: 2020 タイトル: Structural insights into secretory immunoglobulin A and its interaction with a pneumococcal adhesin. 著者: Yuxin Wang / Guopeng Wang / Yaxin Li / Qinyu Zhu / Hao Shen / Ning Gao / Junyu Xiao / 要旨: Secretory Immunoglobulin A (SIgA) is the most abundant antibody at the mucosal surface. It possesses two additional subunits besides IgA: the joining chain (J-chain) and secretory component (SC). SC ...Secretory Immunoglobulin A (SIgA) is the most abundant antibody at the mucosal surface. It possesses two additional subunits besides IgA: the joining chain (J-chain) and secretory component (SC). SC is the ectodomain of the polymeric immunoglobulin receptor (pIgR), which functions to transport IgA to the mucosa. How the J-chain and pIgR/SC facilitate the assembly and secretion of SIgA remains incompletely understood. Furthermore, during the infection of Streptococcus pneumoniae, the pneumococcal adhesin SpsA hijacks pIgR/SC and SIgA to gain entry to human cells and evade host defense. How SpsA targets pIgR/SC and SIgA also remains elusive. Here we report a cryo-electron microscopy structure of the Fc region of IgA1 (Fcα) in complex with the J-chain and SC (Fcα-J-SC), which reveals the organization principle of SIgA. We also present a structure of Fcα-J-SC complexed with SpsA, which uncovers the specific interactions between SpsA and human pIgR/SC. These results advance the molecular understanding of SIgA and shed light on S. pneumoniae pathogenesis. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6lx3.cif.gz | 265.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6lx3.ent.gz | 215.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6lx3.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/lx/6lx3 ftp://data.pdbj.org/pub/pdb/validation_reports/lx/6lx3 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
#1: 抗体 | 分子量: 31445.693 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: IL2, IGHA1 / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: P60568, UniProt: P01876 #2: タンパク質 | | 分子量: 19225.762 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: JCHAIN, IGCJ, IGJ / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: P01591 #3: タンパク質 | | 分子量: 63306.336 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: PIGR / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: P01833 |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: Ternary complex of IgA-Fc with the J chain and pIgR/SC タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
由来(組換発現) | 生物種: Homo sapiens (ヒト) |
緩衝液 | pH: 7.4 |
試料 | 濃度: 0.3 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 277.15 K |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company | |||||||||||||||
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顕微鏡 | モデル: FEI TITAN KRIOS | |||||||||||||||
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM | |||||||||||||||
電子レンズ | モード: BRIGHT FIELD | |||||||||||||||
撮影 |
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-解析
ソフトウェア | 名称: PHENIX / バージョン: 1.15.2_3472: / 分類: 精密化 | ||||||||||||||||||||||||
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
対称性 | 点対称性: C1 (非対称) | ||||||||||||||||||||||||
3次元再構成 | 解像度: 3.15 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 665589 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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