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- PDB-6lbi: Crystal Structure of FOXO1-DBD homodimer bound to a palindromic D... -

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Basic information

Entry
Database: PDB / ID: 6lbi
TitleCrystal Structure of FOXO1-DBD homodimer bound to a palindromic DNA sequence
Components
  • Forkhead box protein O1
  • IRE0
KeywordsDNA BINDING PROTEIN / Transcription factor / FOXO1 / protein-DNA complex / DNA binding
Function / homology
Function and homology information


cellular response to hyperoxia / regulation of transcription initiation by RNA polymerase II / AKT-mediated inactivation of FOXO1A / FOXO-mediated transcription of cell cycle genes / AKT phosphorylates targets in the nucleus / positive regulation of smooth muscle cell apoptotic process / neuronal stem cell population maintenance / regulation of neural precursor cell proliferation / response to fatty acid / Regulation of FOXO transcriptional activity by acetylation ...cellular response to hyperoxia / regulation of transcription initiation by RNA polymerase II / AKT-mediated inactivation of FOXO1A / FOXO-mediated transcription of cell cycle genes / AKT phosphorylates targets in the nucleus / positive regulation of smooth muscle cell apoptotic process / neuronal stem cell population maintenance / regulation of neural precursor cell proliferation / response to fatty acid / Regulation of FOXO transcriptional activity by acetylation / regulation of reactive oxygen species metabolic process / negative regulation of stress-activated MAPK cascade / negative regulation of cardiac muscle hypertrophy in response to stress / cellular response to cold / FOXO-mediated transcription of cell death genes / temperature homeostasis / negative regulation of fat cell differentiation / protein acetylation / blood vessel development / fat cell differentiation / Constitutive Signaling by AKT1 E17K in Cancer / intracellular glucose homeostasis / Regulation of gene expression in beta cells / Regulation of localization of FOXO transcription factors / cellular response to nitric oxide / canonical Wnt signaling pathway / negative regulation of insulin secretion / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / positive regulation of autophagy / energy homeostasis / positive regulation of gluconeogenesis / cellular response to starvation / protein phosphatase 2A binding / promoter-specific chromatin binding / MAPK6/MAPK4 signaling / negative regulation of canonical Wnt signaling pathway / chromatin DNA binding / beta-catenin binding / autophagy / DNA-binding transcription repressor activity, RNA polymerase II-specific / positive regulation of protein catabolic process / cellular response to insulin stimulus / insulin receptor signaling pathway / gene expression / cellular response to oxidative stress / DNA-binding transcription activator activity, RNA polymerase II-specific / Interleukin-4 and Interleukin-13 signaling / sequence-specific DNA binding / nucleic acid binding / DNA-binding transcription factor activity, RNA polymerase II-specific / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / apoptotic process / ubiquitin protein ligase binding / DNA damage response / chromatin binding / chromatin / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / mitochondrion / nucleoplasm / nucleus / cytoplasm / cytosol
Similarity search - Function
: / FOXO protein, KIX-binding domain / KIX-binding domain of forkhead box O, CR2 / FOXO protein, transactivation domain / Transactivation domain of FOXO protein family / Fork head domain / Forkhead domain / Fork head domain profile. / FORKHEAD / Fork head domain conserved site 2 ...: / FOXO protein, KIX-binding domain / KIX-binding domain of forkhead box O, CR2 / FOXO protein, transactivation domain / Transactivation domain of FOXO protein family / Fork head domain / Forkhead domain / Fork head domain profile. / FORKHEAD / Fork head domain conserved site 2 / Fork head domain signature 2. / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / Forkhead box protein O1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.067 Å
AuthorsLi, J. / Dai, S.Y. / Chen, Y.H.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China81570537 China
CitationJournal: Nucleic Acids Res. / Year: 2021
Title: Mechanism of forkhead transcription factors binding to a novel palindromic DNA site.
Authors: Li, J. / Dai, S. / Chen, X. / Liang, X. / Qu, L. / Jiang, L. / Guo, M. / Zhou, Z. / Wei, H. / Zhang, H. / Chen, Z. / Chen, L. / Chen, Y.
History
DepositionNov 14, 2019Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Feb 10, 2021Provider: repository / Type: Initial release
Revision 1.1Sep 8, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2Nov 22, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: IRE0
B: IRE0
C: Forkhead box protein O1
D: Forkhead box protein O1
E: IRE0
F: IRE0
G: Forkhead box protein O1
H: Forkhead box protein O1
I: IRE0
J: IRE0
K: Forkhead box protein O1
L: Forkhead box protein O1


Theoretical massNumber of molelcules
Total (without water)116,12312
Polymers116,12312
Non-polymers00
Water00
1
A: IRE0
B: IRE0
C: Forkhead box protein O1
D: Forkhead box protein O1


Theoretical massNumber of molelcules
Total (without water)38,7084
Polymers38,7084
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5370 Å2
ΔGint-43 kcal/mol
Surface area14130 Å2
MethodPISA
2
E: IRE0
F: IRE0
G: Forkhead box protein O1
H: Forkhead box protein O1


Theoretical massNumber of molelcules
Total (without water)38,7084
Polymers38,7084
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5220 Å2
ΔGint-40 kcal/mol
Surface area14370 Å2
MethodPISA
3
I: IRE0
J: IRE0
K: Forkhead box protein O1
L: Forkhead box protein O1


Theoretical massNumber of molelcules
Total (without water)38,7084
Polymers38,7084
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5250 Å2
ΔGint-39 kcal/mol
Surface area14180 Å2
MethodPISA
Unit cell
Length a, b, c (Å)67.732, 89.090, 194.166
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

#1: DNA chain
IRE0


Mass: 6132.991 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#2: Protein
Forkhead box protein O1 / Forkhead box protein O1A / Forkhead in rhabdomyosarcoma


Mass: 13220.834 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FOXO1, FKHR, FOXO1A / Production host: Escherichia coli (E. coli) / References: UniProt: Q12778

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.52 Å3/Da / Density % sol: 51.24 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop
Details: 50 mM NaAc, pH 4.7, 12%-18% PEG4000 (w/v), 200 mM NaCl, 10 mM MgCl2 and 1 mM TCEP.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: Y
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97915 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 25, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97915 Å / Relative weight: 1
ReflectionResolution: 3.06→46.793 Å / Num. obs: 17379 / % possible obs: 99 % / Redundancy: 10.1 % / Biso Wilson estimate: 26.62 Å2 / Rpim(I) all: 0.107 / Net I/σ(I): 8.8
Reflection shellResolution: 3.06→3.17 Å / Num. unique obs: 314 / Rpim(I) all: 1.208
Serial crystallography sample deliveryMethod: fixed target

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Processing

Software
NameVersionClassification
PHENIX1.11.1_2575refinement
HKL-20001.11.1_2575data processing
HKL-2000data collection
PDB_EXTRACT1.11.1_2575data extraction
HKL-20003.25data reduction
PHENIXphasing
Cootmodel building
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3CO6

3co6


Resolution: 3.067→46.793 Å / SU ML: 0.38 / Cross valid method: THROUGHOUT / σ(F): 1.97 / Phase error: 26.78 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2641 866 5.01 %
Rwork0.2267 16434 -
obs0.2286 17300 76.11 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 103.73 Å2 / Biso mean: 43.6204 Å2 / Biso min: 8.31 Å2
Refinement stepCycle: final / Resolution: 3.067→46.793 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3730 2442 0 0 6172
Num. residues----598
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0036564
X-RAY DIFFRACTIONf_angle_d0.4679409
X-RAY DIFFRACTIONf_chiral_restr0.031030
X-RAY DIFFRACTIONf_plane_restr0.002757
X-RAY DIFFRACTIONf_dihedral_angle_d19.9673363
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.0675-3.25960.3265410.269378922
3.2596-3.51120.35651060.2426190654
3.5112-3.86440.29361600.2565281780
3.8644-4.42330.2361800.2207355299
4.4233-5.57140.25131830.21333637100
5.5714-46.7930.23861960.2132373399
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.71831.64640.29792.8878-0.14341.1009-0.20730.53540.2645-0.61240.28090.3328-0.3056-0.38150.04630.4730.2491-0.08340.46130.0708-0.0041-0.387910.5085-31.1849
20.4071-0.1463-0.45820.09090.18040.51740.1394-0.09450.07520.1141-0.0343-0.0832-0.06930.0642-0.02920.3293-0.15060.15390.0978-0.10220.25913.032416.6474-14.9759
33.3971-2.1811.83443.5805-0.25434.0025-0.0428-0.2294-0.35640.330.05890.01890.32990.1659-0.07590.12260.0021-0.05680.18570.13120.1597-4.53638.863-0.0791
40.22220.19920.11540.28250.39620.9073-0.0448-0.3229-0.03880.21420.1086-0.0641-0.04050.0749-0.01930.6820.2125-0.17220.6560.10560.24834.244511.77717.0845
53.8554-0.8768-1.90841.4465-0.03912.22-0.2627-0.6244-0.38160.35560.27370.23630.594-0.12280.0890.5639-0.2281-0.00130.50620.14790.099-3.56813.573316.1562
60.85320.21320.29220.58211.26542.76740.0734-0.05440.1582-0.02490.1619-0.0858-0.2180.0357-0.05470.05430.0237-0.01570.141-0.07480.2026-0.464611.7446-7.5349
72.2349-0.6933-1.30850.47690.28992.13660.27110.23280.2297-0.4131-0.0299-0.1896-0.00580.274-0.10080.6961-0.09770.18940.76740.09490.16385.435816.2467-31.7308
81.93751.3468-2.82190.9361-1.96134.10990.6645-0.7860.97660.60550.408-0.4105-0.66590.9155-1.01860.79130.3908-0.05271.0929-0.41240.7071-8.787625.052315.3349
90.4591-0.158-0.5041.22950.23930.6929-0.042-0.04490.0056-0.0223-0.0269-0.044-0.0206-0.0153-0.01360.25910.3006-0.03330.3335-0.06960.1572-16.056220.95358.4172
106.2716-2.6749-4.82783.77443.23664.24240.12720.2758-0.13250.0178-0.21060.3826-0.1474-0.28560.03980.18290.03390.15560.08860.04370.2873-22.955820.5057-0.3249
113.16840.51992.83876.29310.754.4714-0.144-0.59980.38690.3273-0.26560.1889-0.4018-0.43150.36130.0762-0.0784-0.05820.326-0.04360.0565-12.495325.89381.0765
123.4283-2.3506-1.86694.10651.79441.1226-0.1019-0.58940.8554-0.58730.4159-0.4721-0.60290.5049-0.28930.7227-0.0832-0.32910.4413-0.19380.8961-3.538730.21093.9193
131.4813-0.702-0.00140.4699-0.03591.6762-0.0728-0.1009-0.08860.0584-0.0108-0.03620.0150.0029-0.03650.14550.18190.04740.1010.1030.0438-5.529316.96044.0497
144.0259-0.22712.34030.2863-0.24061.4028-0.0924-0.2657-0.0460.1940.12990.08390.06930.11480.00750.32510.15920.19440.27870.09250.2754-15.824410.36874.1192
153.66893.6568-0.48123.6795-0.07184.81350.70360.63170.2806-0.72130.17651.2213-0.6318-0.8989-0.85980.34990.12460.02930.76830.05710.6941-17.696114.5281-11.2465
167.6193-4.82782.75716.4897-0.54078.3989-0.2362-0.7537-0.60380.65980.50580.59660.1055-0.9738-0.19620.18760.05590.04930.4837-0.07730.256-20.229612.34492.3839
170.2734-0.1838-0.13280.12350.08930.06460.03640.0422-0.0245-0.0325-0.0093-0.04530.09080.03450.02120.9210.19340.36181.0121-0.30650.68241.0293-1.9916-30.8657
181.353-0.5123-0.81541.7863-0.2351.8191-0.12470.178-0.2964-0.0924-0.08230.4680.4263-0.15560.15880.33340.0624-0.0870.0986-0.11330.2598-7.9592-2.7228-24.8056
195.7847-0.88735.55844.3664-0.21925.86370.0941-0.6991-0.9565-0.09160.00481.6350.1096-0.8457-0.04040.21650.1463-0.04310.3963-0.0330.5577-14.5581-5.6455-15.5439
200.78141.12510.85832.09330.97761.3299-0.02920.1876-0.5293-0.12860.1104-0.39470.24480.0138-0.08260.1205-0.08390.01010.1642-0.09320.5253-2.8542-5.3613-17.024
210.044-0.3688-0.28273.38022.05252.1368-0.01930.2482-0.5692-0.158-0.1045-0.52550.55260.24320.01860.2730.0570.06370.2211-0.08030.59356.5015-4.5523-19.2611
220.0735-0.0969-0.09391.1022-0.04590.1448-0.0451-0.04410.1315-0.0404-0.07960.1459-0.0223-0.0073-0.00740.0612-0.184-0.10330.2392-0.03740.1762-1.65026.264-19.5061
230.9229-0.1019-0.29570.0170.06750.23810.01470.1210.0369-0.0467-0.00680.0449-0.0136-0.0640.0225-0.00680.0004-0.27470.07070.04120.3829-13.38756.3659-20.4696
241.8633-0.81851.13291.9996-0.36070.6904-0.3159-1.1305-1.10021.00990.54110.25380.42270.0914-0.27210.33730.1652-0.09930.46060.15130.6149-13.69161.1761-5.3915
255.38036.0120.49717.65732.13564.1449-0.06940.1650.2032-0.34160.10330.81480.1628-0.2664-0.03580.1427-0.0087-0.02550.1156-0.08190.5976-16.36832.4751-19.4801
261.44580.3362.78370.31580.46995.4885-0.1189-0.11170.0231-0.21320.13480.05130.1008-0.66840.01660.99560.14270.01460.84550.06330.2235-29.5595-13.4465-63.342
270.890.34940.4451.6182-0.40920.81660.0548-0.22990.18620.24810.13060.13470.1331-0.2049-0.03770.6190.08030.30250.7396-0.07340.2976-34.1226-6.9941-47.711
281.8589-1.98713.52974.8741-2.45558.2537-0.4831-0.0831-0.04450.2639-0.0899-0.37760.20660.21260.43430.25770.06360.06070.67690.0010.155-31.6393-16.7356-32.3514
290.1742-0.728-0.24723.0671.27566.5127-0.0974-0.36180.15760.68460.4536-0.1887-0.13650.7689-0.20950.7042-0.1337-0.01090.98180.00750.1961-30.1747-6.7117-15.5029
302.67360.10441.20413.7408-1.84285.42060.363-0.2717-0.25840.7901-0.1039-0.37721.34280.7818-0.24470.6140.0017-0.1650.586-0.15080.2547-35.3899-12.9788-16.5572
310.9736-0.0549-0.58810.01350.03620.34960.26280.41020.119-0.4438-0.1422-0.0599-0.52650.0644-0.03130.7412-0.15490.1350.60660.03450.1687-31.7353-9.9461-48.1519
321.1088-1.3165-1.21382.58780.62662.43030.13610.24460.0581-0.43460.0778-0.4875-0.3799-0.0614-0.18020.9283-0.08060.21850.6799-0.33580.6001-47.871-11.0028-17.0909
331.7540.2975-1.81432.48830.28762.65310.01360.02210.06320.2025-0.13290.3250.1478-0.4640.14280.2036-0.0370.12970.5427-0.12530.2219-48.2785-20.6997-24.0042
345.57572.799-2.71024.349-2.08961.5137-0.0268-0.0871-0.41750.01440.07640.21750.2288-0.244-0.02180.2061-0.17230.10370.1732-0.01350.238-50.5648-27.0179-32.9094
351.3621-0.1218-0.38590.19630.01320.27170.0129-0.03630.020.00920.05010.19330.0083-0.1199-0.0137-0.0713-0.15580.10630.3972-0.050.3239-50.2215-15.3051-31.9502
361.44050.3771-1.26130.2358-0.32042.83590.0480.02920.7446-0.3152-0.10980.3519-1.49290.28770.0431.0369-0.13650.05320.4967-0.12040.6627-49.6285-4.5695-29.4431
373.44481.21790.81042.65660.30023.1402-0.05090.0676-0.34-0.0064-0.0844-0.3996-0.12370.26760.1160.106-0.07360.01370.24440.03740.0904-39.2272-13.4968-28.602
382.3803-0.190.64224.79580.49063.6507-0.08010.454-0.8731-0.46080.1164-0.26960.63520.1945-0.07660.47490.00540.09360.2306-0.14520.5806-40.3885-25.9656-35.351
390.48050.95020.60691.95570.90141.91260.0940.0361-0.15030.15090.0193-0.24170.2636-0.0049-0.12920.2846-0.14340.13920.342-0.32080.5473-43.144-27.4713-31.2697
403.3998-1.2783-1.57941.29951.40242.6068-0.1742-0.1936-0.29980.13120.04650.00450.3750.1830.19540.57610.3410.31020.54270.05830.5309-20.4036-23.163-57.8161
411.1799-0.5926-0.28680.81010.54750.3882-0.0718-0.0023-0.2922-0.16360.0431-0.20480.63520.13590.03770.63290.1149-0.0380.20730.10950.7171-19.4929-26.3563-47.8742
424.4327-0.99252.29084.5377-0.9821.235-0.0676-0.25110.3649-0.24970.0957-1.11550.12740.727-0.04960.3871-0.04680.18630.75490.06090.8403-13.5147-13.9467-51.1548
433.09350.14120.07468.1063-2.38668.6119-0.164-0.0179-0.4464-0.3663-0.08750.34160.0412-0.17540.25590.35190.09680.07670.489-0.01370.2775-29.5921-21.7156-50.2191
441.2156-1.30240.31961.730.59348.41570.2561-0.4356-0.5868-0.1311-0.19330.03871.8315-0.4487-0.04640.7003-0.03640.00070.50310.25450.8206-28.9716-29.1086-45.861
452.19240.05820.73760.67790.36221.3773-0.0548-0.7069-0.33990.56970.0572-0.0290.3767-0.0450.01690.78490.0244-0.07310.76680.16270.1412-1.4233-30.9809-0.8674
462.3621.08350.75461.57820.09380.30.15120.49010.0795-0.02380.18030.0629-0.0777-0.1411-0.14170.4703-0.05760.15560.6990.03930.07625.9586-29.9972-17.0016
470.4508-1.0181-0.90032.40951.56283.91820.13390.6309-0.118-0.52430.0340.1587-0.5645-0.633-0.10070.5499-0.2475-0.13670.9011-0.04370.2447-0.5687-29.1209-40.8864
480.759-0.44030.18791.4696-0.99471.42440.20610.3025-0.1339-0.35860.01020.08730.1626-0.20110.12570.4722-0.14960.00780.7271-0.2346-0.11481.001-29.3729-40.2873
492.6429-2.94673.15824.0876-2.31225.59210.0024-0.3989-0.34150.2925-0.4167-0.23950.16070.3520.2820.5266-0.06580.01880.40670.20170.3505-0.8682-35.3199-17.0374
502.467-0.3721-2.79470.64141.84846.64460.396-0.60230.03910.09110.0114-0.0111-0.01150.1598-0.26870.9330.08490.08330.77850.10410.13816.4453-28.8033-0.1253
510.6186-0.0875-0.08440.61760.12960.2620.08170.07560.08-0.02740.042-0.2882-0.0931-0.0456-0.1261.19430.10580.17490.8322-0.4640.80128.9178-43.2155-47.9487
527.69963.90482.2443.57540.30963.57910.7879-0.3278-0.8642-0.2089-0.8780.13430.598-0.4550.06021.011-0.01130.01720.4382-0.27830.9481-0.6005-50.8755-37.4895
533.0177-2.7027-0.33864.92712.32691.921-0.12340.131-1.0435-0.23720.0053-0.50230.6732-0.17110.13640.9293-0.2146-0.0940.3247-0.03810.87336.7101-47.9034-33.226
541.75481.5661-0.5241.6583-0.81520.61930.87970.5221-0.23980.1651-0.6149-0.7134-0.0812-0.01-0.2580.84680.23980.28090.64360.01680.834213.658-41.4504-34.8126
551.42780.6251.93514.07931.09024.0542-0.15670.05630.09110.1304-0.11140.002-0.2037-0.08370.14630.1967-0.1232-0.01190.3211-0.20540.32062.0601-37.2519-36.5788
560.31730.5196-0.83920.8469-1.36792.20760.073-0.0269-0.03950.07-0.0123-0.02560.2964-0.1683-0.0350.5645-0.2651-0.13660.4472-0.15850.9925-7.3736-44.1045-31.9329
571.40251.41971.12141.57881.5742.25370.0729-0.0593-0.1743-0.06640.1318-0.22320.2945-0.0326-0.18650.8839-0.3368-0.22520.6662-0.32821.0214-7.6653-47.8743-34.5128
582.6941-0.3619-2.29412.61560.86443.4587-0.0352-0.35510.04320.40540.07750.4285-0.0287-0.2042-0.05860.47780.08210.35450.93120.07330.6103-13.94-26.3674-4.1911
594.6452-3.9759-1.84863.40261.59454.21880.08110.4948-0.16030.03820.10270.65180.0553-0.5787-0.13060.2220.00630.09820.90040.30070.5746-17.8872-27.1524-15.8027
604.72912.2767-1.54824.3471-5.43047.2576-0.3057-1.26320.09891.09970.05260.588-0.0162-0.3880.22180.41550.05520.16950.4636-0.14710.3014-10.2921-15.7777-10.8483
610.68170.450.03420.5479-0.14131.56740.0951-0.3308-0.15280.4034-0.0698-0.016-0.2716-0.03970.01910.28350.05230.00790.60060.18740.117-5.3386-28.7792-12.7517
621.91680.84691.25031.54990.04692.5078-0.2340.3376-0.2460.09130.13420.47290.318-0.51880.05670.5155-0.28170.22690.80190.13880.7845-13.8637-38.5342-16.4887
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 5 )A1 - 5
2X-RAY DIFFRACTION2chain 'A' and (resid 6 through 10 )A6 - 10
3X-RAY DIFFRACTION3chain 'A' and (resid 11 through 15 )A11 - 15
4X-RAY DIFFRACTION4chain 'A' and (resid 16 through 20 )A16 - 20
5X-RAY DIFFRACTION5chain 'B' and (resid 1 through 5 )B1 - 5
6X-RAY DIFFRACTION6chain 'B' and (resid 6 through 15 )B6 - 15
7X-RAY DIFFRACTION7chain 'B' and (resid 16 through 20 )B16 - 20
8X-RAY DIFFRACTION8chain 'C' and (resid 159 through 164 )C159 - 164
9X-RAY DIFFRACTION9chain 'C' and (resid 165 through 175 )C165 - 175
10X-RAY DIFFRACTION10chain 'C' and (resid 176 through 182 )C176 - 182
11X-RAY DIFFRACTION11chain 'C' and (resid 183 through 193 )C183 - 193
12X-RAY DIFFRACTION12chain 'C' and (resid 194 through 206 )C194 - 206
13X-RAY DIFFRACTION13chain 'C' and (resid 207 through 219 )C207 - 219
14X-RAY DIFFRACTION14chain 'C' and (resid 220 through 226 )C220 - 226
15X-RAY DIFFRACTION15chain 'C' and (resid 227 through 235 )C227 - 235
16X-RAY DIFFRACTION16chain 'C' and (resid 236 through 242 )C236 - 242
17X-RAY DIFFRACTION17chain 'D' and (resid 160 through 164 )D160 - 164
18X-RAY DIFFRACTION18chain 'D' and (resid 165 through 175 )D165 - 175
19X-RAY DIFFRACTION19chain 'D' and (resid 176 through 182 )D176 - 182
20X-RAY DIFFRACTION20chain 'D' and (resid 183 through 193 )D183 - 193
21X-RAY DIFFRACTION21chain 'D' and (resid 194 through 207 )D194 - 207
22X-RAY DIFFRACTION22chain 'D' and (resid 208 through 219 )D208 - 219
23X-RAY DIFFRACTION23chain 'D' and (resid 220 through 226 )D220 - 226
24X-RAY DIFFRACTION24chain 'D' and (resid 227 through 235 )D227 - 235
25X-RAY DIFFRACTION25chain 'D' and (resid 236 through 243 )D236 - 243
26X-RAY DIFFRACTION26chain 'E' and (resid 1 through 5 )E1 - 5
27X-RAY DIFFRACTION27chain 'E' and (resid 6 through 10 )E6 - 10
28X-RAY DIFFRACTION28chain 'E' and (resid 11 through 15 )E11 - 15
29X-RAY DIFFRACTION29chain 'E' and (resid 16 through 20 )E16 - 20
30X-RAY DIFFRACTION30chain 'F' and (resid 1 through 5 )F1 - 5
31X-RAY DIFFRACTION31chain 'F' and (resid 6 through 20 )F6 - 20
32X-RAY DIFFRACTION32chain 'G' and (resid 158 through 164 )G158 - 164
33X-RAY DIFFRACTION33chain 'G' and (resid 165 through 175 )G165 - 175
34X-RAY DIFFRACTION34chain 'G' and (resid 176 through 182 )G176 - 182
35X-RAY DIFFRACTION35chain 'G' and (resid 183 through 193 )G183 - 193
36X-RAY DIFFRACTION36chain 'G' and (resid 194 through 206 )G194 - 206
37X-RAY DIFFRACTION37chain 'G' and (resid 207 through 219 )G207 - 219
38X-RAY DIFFRACTION38chain 'G' and (resid 220 through 235 )G220 - 235
39X-RAY DIFFRACTION39chain 'G' and (resid 236 through 240 )G236 - 240
40X-RAY DIFFRACTION40chain 'H' and (resid 158 through 175 )H158 - 175
41X-RAY DIFFRACTION41chain 'H' and (resid 176 through 193 )H176 - 193
42X-RAY DIFFRACTION42chain 'H' and (resid 194 through 208 )H194 - 208
43X-RAY DIFFRACTION43chain 'H' and (resid 209 through 229 )H209 - 229
44X-RAY DIFFRACTION44chain 'H' and (resid 233 through 240 )H233 - 240
45X-RAY DIFFRACTION45chain 'I' and (resid 1 through 5 )I1 - 5
46X-RAY DIFFRACTION46chain 'I' and (resid 6 through 10 )I6 - 10
47X-RAY DIFFRACTION47chain 'I' and (resid 11 through 20 )I11 - 20
48X-RAY DIFFRACTION48chain 'J' and (resid 1 through 10 )J1 - 10
49X-RAY DIFFRACTION49chain 'J' and (resid 11 through 15 )J11 - 15
50X-RAY DIFFRACTION50chain 'J' and (resid 16 through 20 )J16 - 20
51X-RAY DIFFRACTION51chain 'K' and (resid 158 through 164 )K158 - 164
52X-RAY DIFFRACTION52chain 'K' and (resid 165 through 182 )K165 - 182
53X-RAY DIFFRACTION53chain 'K' and (resid 183 through 193 )K183 - 193
54X-RAY DIFFRACTION54chain 'K' and (resid 194 through 207 )K194 - 207
55X-RAY DIFFRACTION55chain 'K' and (resid 208 through 219 )K208 - 219
56X-RAY DIFFRACTION56chain 'K' and (resid 220 through 235 )K220 - 235
57X-RAY DIFFRACTION57chain 'K' and (resid 236 through 241 )K236 - 241
58X-RAY DIFFRACTION58chain 'L' and (resid 156 through 175 )L156 - 175
59X-RAY DIFFRACTION59chain 'L' and (resid 176 through 193 )L176 - 193
60X-RAY DIFFRACTION60chain 'L' and (resid 194 through 206 )L194 - 206
61X-RAY DIFFRACTION61chain 'L' and (resid 207 through 219 )L207 - 219
62X-RAY DIFFRACTION62chain 'L' and (resid 220 through 240 )L220 - 240

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