+
Open data
-
Basic information
Entry | Database: PDB / ID: 6kvn | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of chloroplast resolvase | ||||||
![]() | NtMOC1 | ||||||
![]() | PLANT PROTEIN / chloroplast / resolvase | ||||||
Function / homology | Holliday junction resolvase MOC1-like / crossover junction DNA endonuclease activity / metal ion binding / Uncharacterized protein LOC107823122![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yan, J.J. / Hong, S.X. / Guan, Z.Y. / Yin, P. | ||||||
![]() | ![]() Title: Structural insights into sequence-dependent Holliday junction resolution by the chloroplast resolvase MOC1. Authors: Yan, J. / Hong, S. / Guan, Z. / He, W. / Zhang, D. / Yin, P. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 87.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 55.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.4 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 422.6 KB | Display | |
Data in XML | ![]() | 8.2 KB | Display | |
Data in CIF | ![]() | 10.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||||||
Unit cell |
| ||||||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 18605.326 Da / Num. of mol.: 1 / Mutation: Q162K,E235Q,E239Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.27 % |
---|---|
Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, hanging drop / Details: Bis-Tris, PEG 3350, Lithium sulphat |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 13, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 2.02→43.57 Å / Num. obs: 10538 / % possible obs: 99.8 % / Redundancy: 6.3 % / Biso Wilson estimate: 29.46 Å2 / CC1/2: 0.978 / Rmerge(I) obs: 0.207 / Rpim(I) all: 0.09 / Rrim(I) all: 0.226 / Net I/σ(I): 6 |
Reflection shell | Resolution: 2.02→2.07 Å / Redundancy: 5.7 % / Rmerge(I) obs: 0.853 / Mean I/σ(I) obs: 2.6 / Num. unique obs: 4218 / CC1/2: 0.778 / Rpim(I) all: 0.387 / Rrim(I) all: 0.94 / % possible all: 98.3 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 42.33 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.02→43.57 Å
| ||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| ||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: -10.2845034781 Å / Origin y: 11.2041636706 Å / Origin z: -16.4450170179 Å
| ||||||||||||||||||||||||||||||||||||||||
Refinement TLS group | Selection details: all |