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Open data
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Basic information
Entry | Database: PDB / ID: 6k03 | ||||||
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Title | Crystal structure of ceH2A-H2B | ||||||
![]() | Histone H2B 1,Histone H2A | ||||||
![]() | CHAPERONE / H2A-H2B | ||||||
Function / homology | ![]() Metalloprotease DUBs / B-WICH complex positively regulates rRNA expression / Assembly of the ORC complex at the origin of replication / Ub-specific processing proteases / Oxidative Stress Induced Senescence / Transcriptional regulation by small RNAs / RNA Polymerase I Promoter Escape / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / Condensation of Prophase Chromosomes / RMTs methylate histone arginines ...Metalloprotease DUBs / B-WICH complex positively regulates rRNA expression / Assembly of the ORC complex at the origin of replication / Ub-specific processing proteases / Oxidative Stress Induced Senescence / Transcriptional regulation by small RNAs / RNA Polymerase I Promoter Escape / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / Condensation of Prophase Chromosomes / RMTs methylate histone arginines / heterochromatin organization / nucleosomal DNA binding / structural constituent of chromatin / nucleosome / protein heterodimerization activity / DNA repair / protein-containing complex binding / regulation of DNA-templated transcription / chromatin / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, Y.R. | ||||||
![]() | ![]() Title: Crystal structure of xlH2A-H2B Authors: Liu, Y.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 78 KB | Display | ![]() |
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PDB format | ![]() | 56.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422 KB | Display | ![]() |
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Full document | ![]() | 423.9 KB | Display | |
Data in XML | ![]() | 7.9 KB | Display | |
Data in CIF | ![]() | 9.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6k00C ![]() 6k01SC ![]() 6k02C ![]() 6k09C ![]() 6k0cC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22653.262 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.82 Å3/Da / Density % sol: 32.36 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / Details: 20% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 18, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9789 Å / Relative weight: 1 |
Reflection | Resolution: 2.857→44.39 Å / Num. obs: 3967 / % possible obs: 97.5 % / Redundancy: 5.4 % / Net I/σ(I): 12.15 |
Reflection shell | Resolution: 2.857→2.96 Å / Rmerge(I) obs: 0.68 / Num. unique obs: 3967 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6K01 Resolution: 2.857→43.394 Å / SU ML: 0.44 / Cross valid method: THROUGHOUT / σ(F): 1.39 / Phase error: 29.74
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 105.41 Å2 / Biso mean: 50.6172 Å2 / Biso min: 27.77 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.857→43.394 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 3
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Refinement TLS params. | Method: refined / Origin x: -19.7115 Å / Origin y: 13.1587 Å / Origin z: 1.9542 Å
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Refinement TLS group | Selection details: (chain 'D' and resid 30 through 212) |