+Open data
-Basic information
Entry | Database: PDB / ID: 6j7g | ||||||
---|---|---|---|---|---|---|---|
Title | Human H-ferritin mutant-C90A/C102A/C130A/D144C | ||||||
Components | Ferritin heavy chain | ||||||
Keywords | OXIDOREDUCTASE / Human ferritin / Disulfide bond | ||||||
Function / homology | Function and homology information iron ion sequestering activity / ferritin complex / Scavenging by Class A Receptors / negative regulation of ferroptosis / Golgi Associated Vesicle Biogenesis / ferroxidase / autolysosome / ferroxidase activity / intracellular sequestering of iron ion / negative regulation of fibroblast proliferation ...iron ion sequestering activity / ferritin complex / Scavenging by Class A Receptors / negative regulation of ferroptosis / Golgi Associated Vesicle Biogenesis / ferroxidase / autolysosome / ferroxidase activity / intracellular sequestering of iron ion / negative regulation of fibroblast proliferation / autophagosome / ferric iron binding / Iron uptake and transport / ferrous iron binding / tertiary granule lumen / iron ion transport / intracellular iron ion homeostasis / ficolin-1-rich granule lumen / immune response / iron ion binding / negative regulation of cell population proliferation / Neutrophil degranulation / extracellular exosome / extracellular region / identical protein binding / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.868 Å | ||||||
Authors | Zang, J. / Chen, H. / Zhang, X. / Zhao, G. | ||||||
Funding support | China, 1items
| ||||||
Citation | Journal: Nat Commun / Year: 2019 Title: Disulfide-mediated conversion of 8-mer bowl-like protein architecture into three different nanocages. Authors: Zang, J. / Chen, H. / Zhang, X. / Zhang, C. / Guo, J. / Du, M. / Zhao, G. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6j7g.cif.gz | 780.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6j7g.ent.gz | 648.4 KB | Display | PDB format |
PDBx/mmJSON format | 6j7g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6j7g_validation.pdf.gz | 625.8 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 6j7g_full_validation.pdf.gz | 664.5 KB | Display | |
Data in XML | 6j7g_validation.xml.gz | 125.1 KB | Display | |
Data in CIF | 6j7g_validation.cif.gz | 171.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j7/6j7g ftp://data.pdbj.org/pub/pdb/validation_reports/j7/6j7g | HTTPS FTP |
-Related structure data
Related structure data | 6ipcC 6ipoC 6ippC 6ipqC 2fhaS S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 20344.531 Da / Num. of mol.: 24 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FTH1, FTH, FTHL6 / Production host: Escherichia coli (E. coli) / References: UniProt: P02794, ferroxidase #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.27 Å3/Da / Density % sol: 62.37 % |
---|---|
Crystal grow | Temperature: 293 K / Method: evaporation / Details: Amonium Sulfate, HEPES |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.9979 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 13, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9979 Å / Relative weight: 1 |
Reflection | Resolution: 3.86→40.87 Å / Num. obs: 54320 / % possible obs: 92 % / Redundancy: 2.3 % / CC1/2: 0.921 / Net I/σ(I): 2.7 |
Reflection shell | Resolution: 3.86→4.9 Å / CC1/2: 0.921 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2FHA Resolution: 3.868→40.87 Å / SU ML: 0.53 / Cross valid method: NONE / σ(F): 1.36 / Phase error: 36.01
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.868→40.87 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|