- PDB-6ii6: Crystal structure of the Makes Caterpillars Floppy (MCF)-Like eff... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 6ii6
Title
Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3)
Mass: 18.015 Da / Num. of mol.: 581 / Source method: isolated from a natural source / Formula: H2O
Sequence details
The sequence of MCF domain has been deposited to database with NCBI accession ID WP_015728045.1. ...The sequence of MCF domain has been deposited to database with NCBI accession ID WP_015728045.1. GAM at N-terminal were from expression tag. Residue C3351S represents mutation.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.41 Å3/Da / Density % sol: 48.89 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 20% PEG 3350, 0.1 M Bis-Tris-HCl (pH 5.5), and 2% Tacsimate (pH 4.0)
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
Resolution: 2.1→50 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.934 / SU B: 4.845 / SU ML: 0.126 / Cross valid method: THROUGHOUT / ESU R: 0.202 / ESU R Free: 0.183 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.23214
3485
5.1 %
RANDOM
Rwork
0.17491
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obs
0.17783
64463
97.96 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å