actin filament-based process / Rab protein signal transduction / plasma membrane to endosome transport / protein localization to endosome / cell projection membrane / clathrin-coated endocytic vesicle / RAB geranylgeranylation / RAB GEFs exchange GTP for GDP on RABs / endocytic recycling / TBC/RABGAPs ...actin filament-based process / Rab protein signal transduction / plasma membrane to endosome transport / protein localization to endosome / cell projection membrane / clathrin-coated endocytic vesicle / RAB geranylgeranylation / RAB GEFs exchange GTP for GDP on RABs / endocytic recycling / TBC/RABGAPs / endosomal transport / intercellular bridge / antigen processing and presentation / mitotic cytokinesis / clathrin-coated pit / ruffle / phosphatidylinositol-4,5-bisphosphate binding / GTPase activator activity / cellular response to nerve growth factor stimulus / clathrin-coated endocytic vesicle membrane / protein localization / recycling endosome membrane / GDP binding / neuron projection development / melanosome / protein transport / endosome membrane / GTPase activity / GTP binding / extracellular exosome / membrane / metal ion binding / plasma membrane / cytosol Similarity search - Function
Rab35 / Arf GTPase activating protein / ArfGAP domain superfamily / Putative GTPase activating protein for Arf / ARF GTPase-activating proteins domain profile. / Putative GTP-ase activating proteins for the small GTPase, ARF / ARFGAP/RecO-like zinc finger / Domain of unknown function DUF3447 / AH/BAR domain superfamily / ARF-like small GTPases; ARF, ADP-ribosylation factor ...Rab35 / Arf GTPase activating protein / ArfGAP domain superfamily / Putative GTPase activating protein for Arf / ARF GTPase-activating proteins domain profile. / Putative GTP-ase activating proteins for the small GTPase, ARF / ARFGAP/RecO-like zinc finger / Domain of unknown function DUF3447 / AH/BAR domain superfamily / ARF-like small GTPases; ARF, ADP-ribosylation factor / small GTPase Rab1 family profile. / Ankyrin repeat-containing domain / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Ankyrin repeats (3 copies) / Rab subfamily of small GTPases / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Small GTP-binding protein domain / PH-like domain superfamily / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 / Ras-related protein Rab-35 Similarity search - Component
Resolution: 1.5→28.049 Å / FOM work R set: 0.8541 / SU ML: 0.18 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 21.6 / Stereochemistry target values: ML
Rfactor
Num. reflection
% reflection
Rfree
0.2051
2759
5.06 %
Rwork
0.1721
51720
-
obs
0.1738
54479
96.78 %
Solvent computation
Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL