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- PDB-6ial: Porcine E.coli heat-labile enterotoxin B-pentamer in complex with... -

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Basic information

Entry
Database: PDB / ID: 6ial
TitlePorcine E.coli heat-labile enterotoxin B-pentamer in complex with Lacto-N-neohexaose
ComponentsHeat-labile enterotoxin B chain
KeywordsTOXIN / porcine / heat-labile enterotoxin / ETEC / E.coli / lectin / complex / X-ray crystal structure / carbohydrate / protein-carbohydrate interaction / ligand / Lacto-N-neohexaose
Function / homology
Function and homology information


toxin activity / killing of cells of another organism / extracellular region
Similarity search - Function
Heat-labile enterotoxin, B chain / Heat-labile enterotoxin beta chain / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #110 / Enterotoxin / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta
Similarity search - Domain/homology
Heat-labile enterotoxin B chain
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.45 Å
AuthorsHeim, J.B. / Heggelund, J.E. / Krengel, U.
Funding support Norway, 3items
OrganizationGrant numberCountry
Research Council of Norway171631 Norway
Research Council of Norway183613 Norway
Research Council of Norway247730 Norway
CitationJournal: Int J Mol Sci / Year: 2019
Title: Specificity ofEscherichia coliHeat-Labile Enterotoxin Investigated by Single-Site Mutagenesis and Crystallography.
Authors: Heggelund, J.E. / Heim, J.B. / Bajc, G. / Hodnik, V. / Anderluh, G. / Krengel, U.
History
DepositionNov 27, 2018Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 27, 2019Provider: repository / Type: Initial release
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_conn_angle / struct_asym / struct_conn / struct_conn_type / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _atom_site_anisotrop.U[1][1] / _atom_site_anisotrop.U[1][2] / _atom_site_anisotrop.U[1][3] / _atom_site_anisotrop.U[2][2] / _atom_site_anisotrop.U[2][3] / _atom_site_anisotrop.U[3][3] / _atom_site_anisotrop.id / _atom_site_anisotrop.pdbx_auth_asym_id / _atom_site_anisotrop.pdbx_auth_atom_id / _atom_site_anisotrop.pdbx_auth_comp_id / _atom_site_anisotrop.pdbx_auth_seq_id / _atom_site_anisotrop.pdbx_label_asym_id / _atom_site_anisotrop.pdbx_label_atom_id / _atom_site_anisotrop.pdbx_label_comp_id / _atom_site_anisotrop.type_symbol / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_alt_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr1_symmetry / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_symmetry / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_alt_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.ptnr3_symmetry / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_ptnr1_label_alt_id / _struct_conn.pdbx_ptnr2_label_alt_id / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr1_symmetry / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry / _struct_conn_type.id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Jan 24, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_ncs_dom_lim
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 2.2Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
D: Heat-labile enterotoxin B chain
E: Heat-labile enterotoxin B chain
F: Heat-labile enterotoxin B chain
G: Heat-labile enterotoxin B chain
H: Heat-labile enterotoxin B chain
A: Heat-labile enterotoxin B chain
B: Heat-labile enterotoxin B chain
C: Heat-labile enterotoxin B chain
I: Heat-labile enterotoxin B chain
J: Heat-labile enterotoxin B chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)120,76729
Polymers118,07510
Non-polymers2,69219
Water11,620645
1
D: Heat-labile enterotoxin B chain
E: Heat-labile enterotoxin B chain
F: Heat-labile enterotoxin B chain
G: Heat-labile enterotoxin B chain
H: Heat-labile enterotoxin B chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,61722
Polymers59,0385
Non-polymers1,57917
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
A: Heat-labile enterotoxin B chain
B: Heat-labile enterotoxin B chain
C: Heat-labile enterotoxin B chain
I: Heat-labile enterotoxin B chain
J: Heat-labile enterotoxin B chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,1517
Polymers59,0385
Non-polymers1,1132
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)77.079, 65.587, 96.266
Angle α, β, γ (deg.)90.00, 108.64, 90.00
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11D
21E
12D
22F
13D
23G
14D
24H
15D
25A
16D
26B
17D
27C
18D
28I
19D
29J
110E
210F
111E
211G
112E
212H
113E
213A
114E
214B
115E
215C
116E
216I
117E
217J
118F
218G
119F
219H
120F
220A
121F
221B
122F
222C
123F
223I
124F
224J
125G
225H
126G
226A
127G
227B
128G
228C
129G
229I
130G
230J
131H
231A
132H
232B
133H
233C
134H
234I
135H
235J
136A
236B
137A
237C
138A
238I
139A
239J
140B
240C
141B
241I
142B
242J
143C
243I
144C
244J
145I
245J

NCS domain segments:

Component-ID: _ / Beg auth comp-ID: ALA / Beg label comp-ID: ALA / End auth comp-ID: ASN / End label comp-ID: ASN / Refine code: _ / Auth seq-ID: 1 - 103 / Label seq-ID: 1 - 103

Dom-IDEns-IDAuth asym-IDLabel asym-ID
11DA
21EB
12DA
22FC
13DA
23GD
14DA
24HE
15DA
25AF
16DA
26BG
17DA
27CH
18DA
28II
19DA
29JJ
110EB
210FC
111EB
211GD
112EB
212HE
113EB
213AF
114EB
214BG
115EB
215CH
116EB
216II
117EB
217JJ
118FC
218GD
119FC
219HE
120FC
220AF
121FC
221BG
122FC
222CH
123FC
223II
124FC
224JJ
125GD
225HE
126GD
226AF
127GD
227BG
128GD
228CH
129GD
229II
130GD
230JJ
131HE
231AF
132HE
232BG
133HE
233CH
134HE
234II
135HE
235JJ
136AF
236BG
137AF
237CH
138AF
238II
139AF
239JJ
140BG
240CH
141BG
241II
142BG
242JJ
143CH
243II
144CH
244JJ
145II
245JJ

NCS ensembles :
ID
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45

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Components

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Protein / Sugars , 2 types, 12 molecules DEFGHABCIJ

#1: Protein
Heat-labile enterotoxin B chain / LT-B / porcine / LTP-B


Mass: 11807.539 Da / Num. of mol.: 10
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: eltB, ltpB / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P32890
#2: Polysaccharide beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-[beta-D-galactopyranose- ...beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-[beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)]beta-D-galactopyranose-(1-4)-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1072.964 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGalpb1-4DGlcpNAcb1-3[DGalpb1-4DGlcpNAcb1-6]DGalpb1-4DGlcpb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,6,5/[a2122h-1b_1-5][a2112h-1b_1-5][a2122h-1b_1-5_2*NCC/3=O]/1-2-3-2-3-2/a4-b1_b3-c1_b6-e1_c4-d1_e4-f1WURCSPDB2Glycan 1.1.0
[][b-D-Glcp]{[(4+1)][b-D-Galp]{[(3+1)][b-D-GlcpNAc]{[(4+1)][b-D-Galp]{}}[(6+1)][b-D-GlcpNAc]{[(4+1)][b-D-Galp]{}}}}LINUCSPDB-CARE

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Non-polymers , 4 types, 662 molecules

#3: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H8O3
#4: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: Na
#5: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Ca
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 645 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.95 Å3/Da / Density % sol: 37.01 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5
Details: 0.1 M sodium acetate, 20 % w/v PEG 6000, 0.2 M lithium chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8729 Å
DetectorType: DECTRIS PILATUS3 X 2M / Detector: PIXEL / Date: Dec 5, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.8729 Å / Relative weight: 1
ReflectionResolution: 1.45→68.73 Å / Num. obs: 160263 / % possible obs: 99.8 % / Redundancy: 4.4 % / Rmerge(I) obs: 0.125 / Net I/σ(I): 6.4
Reflection shellResolution: 1.45→1.47 Å / Rmerge(I) obs: 1.151

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Processing

Software
NameVersionClassification
REFMAC5.8.0232refinement
xia2data reduction
DIALSdata reduction
Aimlessdata scaling
PHASERphasing
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1DJR

1djr


Resolution: 1.45→68.73 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.956 / SU B: 3.2 / SU ML: 0.059 / Cross valid method: THROUGHOUT / ESU R: 0.074 / ESU R Free: 0.074 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.20087 7973 5 %RANDOM
Rwork0.17524 ---
obs0.17653 152246 99.45 %-
Solvent computationIon probe radii: 0.7 Å / Shrinkage radii: 0.7 Å / VDW probe radii: 1.1 Å
Displacement parametersBiso mean: 18.482 Å2
Baniso -1Baniso -2Baniso -3
1-0.14 Å20 Å2-0.04 Å2
2---0.14 Å20 Å2
3---0.02 Å2
Refinement stepCycle: 1 / Resolution: 1.45→68.73 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8130 0 173 645 8948
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.0138961
X-RAY DIFFRACTIONr_bond_other_d0.0010.0178528
X-RAY DIFFRACTIONr_angle_refined_deg1.6821.66612168
X-RAY DIFFRACTIONr_angle_other_deg1.3931.58320024
X-RAY DIFFRACTIONr_dihedral_angle_1_deg15.1875.1881145
X-RAY DIFFRACTIONr_dihedral_angle_2_deg31.51723.756402
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.407151806
X-RAY DIFFRACTIONr_dihedral_angle_4_deg25.9051543
X-RAY DIFFRACTIONr_chiral_restr0.0810.21358
X-RAY DIFFRACTIONr_gen_planes_refined0.0090.0210465
X-RAY DIFFRACTIONr_gen_planes_other0.0020.021640
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it4.5430.8044332
X-RAY DIFFRACTIONr_mcbond_other4.5430.8044331
X-RAY DIFFRACTIONr_mcangle_it5.2621.1795482
X-RAY DIFFRACTIONr_mcangle_other5.2621.1795483
X-RAY DIFFRACTIONr_scbond_it9.341.3774629
X-RAY DIFFRACTIONr_scbond_other9.3391.3774630
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other10.4231.7986677
X-RAY DIFFRACTIONr_long_range_B_refined10.59411.5429975
X-RAY DIFFRACTIONr_long_range_B_other10.59911.3249886
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05

Ens-IDDom-IDAuth asym-IDNumberRms dev position (Å)
11D29790.13
12E29790.13
21D30040.14
22F30040.14
31D29590.13
32G29590.13
41D31010.12
42H31010.12
51D31360.09
52A31360.09
61D29360.15
62B29360.15
71D29620.14
72C29620.14
81D29640.14
82I29640.14
91D30610.12
92J30610.12
101E31690.13
102F31690.13
111E30310.12
112G30310.12
121E30650.14
122H30650.14
131E30170.14
132A30170.14
141E32600.1
142B32600.1
151E31120.14
152C31120.14
161E30160.13
162I30160.13
171E30240.15
172J30240.15
181F29710.11
182G29710.11
191F30120.15
192H30120.15
201F29700.14
202A29700.14
211F30860.13
212B30860.13
221F31930.11
222C31930.11
231F30010.1
232I30010.1
241F30000.14
242J30000.14
251G29700.12
252H29700.12
261G29610.13
262A29610.13
271G29960.12
272B29960.12
281G29620.13
282C29620.13
291G30500.1
292I30500.1
301G29520.12
302J29520.12
311H30760.12
312A30760.12
321H29870.14
322B29870.14
331H29790.15
332C29790.15
341H29310.13
342I29310.13
351H32730.09
352J32730.09
361A29230.15
362B29230.15
371A29350.15
372C29350.15
381A29220.14
382I29220.14
391A30380.12
392J30380.12
401B31040.13
402C31040.13
411B29620.14
412I29620.14
421B29720.15
422J29720.15
431C31330.12
432I31330.12
441C31130.14
442J31130.14
451I29110.13
452J29110.13
LS refinement shellResolution: 1.45→1.488 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.291 584 -
Rwork0.277 11137 -
obs--98.72 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.0988-0.1588-0.04862.60590.16041.8028-0.02020.0694-0.0321-0.09980.028-0.14870.03770.1361-0.00780.00560.00010.00650.0167-0.00360.010429.1385-28.057732.8996
21.00070.1065-0.34241.61050.07432.73640.04050.0478-0.0401-0.0615-0.009-0.18070.10890.2414-0.03150.01950.01140.00590.0438-0.00970.023829.1284-27.675811.0509
32.16490.02050.11772.976-0.34341.59160.03410.15620.0922-0.2198-0.0218-0.2088-0.07920.1644-0.01230.0382-0.00580.02120.06810.01010.020828.1573-6.75644.5325
40.86960.24080.16932.36240.53012.2151-0.0128-0.00030.0710.02840.0088-0.175-0.13660.14650.00410.0279-0.00790.00390.0250.01360.040528.15356.008622.7441
51.4259-0.3020.81721.3586-0.15561.57920.0210.0427-0.02430.0046-0.0064-0.13230.05030.105-0.01460.0104-0.00340.00430.0124-0.00530.018530.8951-7.543940.7652
61.5024-0.06930.03472.2929-0.09771.9979-0.0051-0.0212-0.1396-0.01090.04090.14460.1644-0.1661-0.03580.039-0.0052-0.01080.0498-0.00210.0255-5.8007-27.405311.8139
71.3629-0.1658-0.19241.4154-0.20372.76550.05440.0014-0.05880.0254-0.02940.16320.1155-0.2028-0.02490.0111-0.013-0.00330.01940.00210.0282-5.3807-29.074933.5668
81.11670.05810.10332.37770.56731.5510.0177-0.02450.07140.0479-0.01840.2017-0.0949-0.19540.00080.00990.0150.010.04870.00740.025-5.0643-8.926241.9481
91.12550.00590.31022.3344-0.53412.6716-0.0302-0.02420.1376-0.04020.03830.2234-0.2455-0.2049-0.00810.05890.020200.04580.00260.0667-5.665.598725.3345
101.56610.28530.90190.93390.12851.53340.00420.0282-0.0007-0.06360.00150.1049-0.0318-0.0551-0.00570.05460.0153-0.00240.04220.02190.0322-7.9793-6.16826.024
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1D1 - 301
2X-RAY DIFFRACTION2E1 - 103
3X-RAY DIFFRACTION3F1 - 103
4X-RAY DIFFRACTION4G1 - 103
5X-RAY DIFFRACTION5H1 - 201
6X-RAY DIFFRACTION6A1 - 103
7X-RAY DIFFRACTION7B1 - 103
8X-RAY DIFFRACTION8C1 - 103
9X-RAY DIFFRACTION9I1 - 103
10X-RAY DIFFRACTION10J1 - 201

+
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