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- PDB-6i5p: Co-crystal structure of human SPOP MATH domain (E47K) and human B... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6i5p | ||||||
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Title | Co-crystal structure of human SPOP MATH domain (E47K) and human BRD3 fragment | ||||||
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![]() | LIGASE / ligase nuclear cancer ubiquitination | ||||||
Function / homology | ![]() regulation of proteolysis / lncRNA binding / Cul3-RING ubiquitin ligase complex / molecular function inhibitor activity / endodermal cell differentiation / localization / protein localization to chromatin / molecular condensate scaffold activity / Hedgehog 'on' state / lysine-acetylated histone binding ...regulation of proteolysis / lncRNA binding / Cul3-RING ubiquitin ligase complex / molecular function inhibitor activity / endodermal cell differentiation / localization / protein localization to chromatin / molecular condensate scaffold activity / Hedgehog 'on' state / lysine-acetylated histone binding / protein polyubiquitination / proteasome-mediated ubiquitin-dependent protein catabolic process / nuclear speck / chromatin remodeling / ubiquitin protein ligase binding / chromatin binding / chromatin / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ostertag, M.S. / Popowicz, G.M. / Sattler, M. | ||||||
![]() | ![]() Title: Structural Insights into BET Client Recognition of Endometrial and Prostate Cancer-Associated SPOP Mutants. Authors: Ostertag, M.S. / Hutwelker, W. / Plettenburg, O. / Sattler, M. / Popowicz, G.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 258.4 KB | Display | ![]() |
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PDB format | ![]() | 208.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 480.5 KB | Display | ![]() |
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Full document | ![]() | 486.4 KB | Display | |
Data in XML | ![]() | 28.1 KB | Display | |
Data in CIF | ![]() | 40.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6i41C ![]() 6i68C ![]() 6i7aC ![]() 3ivvS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16616.203 Da / Num. of mol.: 4 / Mutation: E47K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein/peptide | Mass: 935.995 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 52.14 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 200mM Ammonium Acetate, 100mM Tris pH 8.5, 25% (w/v) PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Jun 23, 2018 |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.966 Å / Relative weight: 1 |
Reflection | Resolution: 1.71→45.17 Å / Num. obs: 71635 / % possible obs: 98.6 % / Redundancy: 3.23 % / Rrim(I) all: 0.058 / Net I/σ(I): 11.82 |
Reflection shell | Resolution: 1.71→1.754 Å / Num. unique obs: 11784 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3IVV Resolution: 1.81→19.931 Å / SU ML: 0.25 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 25.78 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.81→19.931 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -1.3348 Å / Origin y: -9.7848 Å / Origin z: 23.0198 Å
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Refinement TLS group | Selection details: all |