+Open data
-Basic information
Entry | Database: PDB / ID: 6hxg | ||||||
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Title | PDX1.2/PDX1.3 complex (intermediate) | ||||||
Components |
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Keywords | PLANT PROTEIN / Swinging arm lysine / Vitamin B6 / Pseudoenzyme / Reaction intermediate | ||||||
Function / homology | Function and homology information response to non-ionic osmotic stress / amine-lyase activity / response to lipid hydroperoxide / chlorophyll metabolic process / pyridoxal 5'-phosphate synthase (glutamine hydrolysing) / pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity / pyridoxal phosphate biosynthetic process / hyperosmotic salinity response / pyridoxine biosynthetic process / amino acid metabolic process ...response to non-ionic osmotic stress / amine-lyase activity / response to lipid hydroperoxide / chlorophyll metabolic process / pyridoxal 5'-phosphate synthase (glutamine hydrolysing) / pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity / pyridoxal phosphate biosynthetic process / hyperosmotic salinity response / pyridoxine biosynthetic process / amino acid metabolic process / response to UV-B / endomembrane system / response to salt stress / response to oxidative stress / protein heterodimerization activity / protein homodimerization activity / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Robinson, G.C. / Kaufmann, M. / Roux, C. / Martinez-Font, J. / Hothorn, M. / Thore, S. / Fitzpatrick, T.B. | ||||||
Funding support | Switzerland, 1items
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Citation | Journal: Acta Crystallogr D Struct Biol / Year: 2019 Title: Crystal structure of the pseudoenzyme PDX1.2 in complex with its cognate enzyme PDX1.3: a total eclipse. Authors: Robinson, G.C. / Kaufmann, M. / Roux, C. / Martinez-Font, J. / Hothorn, M. / Thore, S. / Fitzpatrick, T.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6hxg.cif.gz | 380.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6hxg.ent.gz | 302.9 KB | Display | PDB format |
PDBx/mmJSON format | 6hxg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6hxg_validation.pdf.gz | 507.2 KB | Display | wwPDB validaton report |
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Full document | 6hxg_full_validation.pdf.gz | 546.1 KB | Display | |
Data in XML | 6hxg_validation.xml.gz | 73.4 KB | Display | |
Data in CIF | 6hxg_validation.cif.gz | 97.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hx/6hxg ftp://data.pdbj.org/pub/pdb/validation_reports/hx/6hxg | HTTPS FTP |
-Related structure data
Related structure data | 6hx3C 6hyeC 5k3vS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34703.375 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: PDX12, A37, PDX1L2, At3g16050, MSL1.3 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 / Variant (production host): DE3 (RIL) / References: UniProt: Q9ZNR6 #2: Protein | Mass: 29202.814 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: PDX13, GIP2, PDX1L3, RSR4, At5g01410, T10O8.120 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL References: UniProt: Q8L940, pyridoxal 5'-phosphate synthase (glutamine hydrolysing) #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53.15 % / Description: Rhombohedral morphology |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.8 M ammonium sulphate, 0.05 M MES.OH, pH 6.5, 5% 1,4-dioxane, 0.013 M ammonium sulphate, 0.003 M R5P, 0.001 M G3P, 0.02 M Tris.HCl, pH 8.0, 0.1 M KCl, 0.005 M DTT |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 0.97853 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Sep 19, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97853 Å / Relative weight: 1 |
Reflection | Resolution: 1.84→93.12 Å / Num. obs: 119464 / % possible obs: 99.4 % / Redundancy: 6.8 % / CC1/2: 0.997 / Rmerge(I) obs: 0.126 / Rpim(I) all: 0.087 / Rrim(I) all: 0.149 / Net I/σ(I): 8.1 |
Reflection shell | Resolution: 1.84→1.87 Å / Redundancy: 5.2 % / Rmerge(I) obs: 2.143 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 5824 / CC1/2: 0.085 / Rpim(I) all: 1.502 / Rrim(I) all: 2.639 / % possible all: 97.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Chainsaw model derived from 5K3V Resolution: 1.9→93.12 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.945 / SU B: 7.938 / SU ML: 0.215 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.249 / ESU R Free: 0.172 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 113.71 Å2 / Biso mean: 29.775 Å2 / Biso min: 14.55 Å2
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Refinement step | Cycle: final / Resolution: 1.9→93.12 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.949 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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