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Yorodumi- PDB-6h9n: Complex of the periplasmic domains of bacterial cell division pro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6h9n | |||||||||
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Title | Complex of the periplasmic domains of bacterial cell division proteins FtsQ and FtsB | |||||||||
Components |
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Keywords | CELL CYCLE / bacterial cell division / divisome | |||||||||
Function / homology | Function and homology information FtsBL complex / FtsQBL complex / divisome complex / cell septum / FtsZ-dependent cytokinesis / division septum assembly / cell division site / plasma membrane => GO:0005886 / cell division / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Escherichia coli K-12 (bacteria) Escherichia coli 55989 (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | |||||||||
Authors | Kureisaite-Ciziene, D. / Lowe, J. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: MBio / Year: 2018 Title: Structural Analysis of the Interaction between the Bacterial Cell Division Proteins FtsQ and FtsB. Authors: Kureisaite-Ciziene, D. / Varadajan, A. / McLaughlin, S.H. / Glas, M. / Monton Silva, A. / Luirink, R. / Mueller, C. / den Blaauwen, T. / Grossmann, T.N. / Luirink, J. / Lowe, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6h9n.cif.gz | 62.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6h9n.ent.gz | 43.2 KB | Display | PDB format |
PDBx/mmJSON format | 6h9n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h9/6h9n ftp://data.pdbj.org/pub/pdb/validation_reports/h9/6h9n | HTTPS FTP |
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-Related structure data
Related structure data | 6h9oC 2vh1S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 26269.643 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Gene: ftsQ, b0093, JW0091 / Production host: Escherichia coli (E. coli) / References: UniProt: P06136 |
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#2: Protein | Mass: 11433.473 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli 55989 (bacteria) / Gene: ftsB, EC55989_3020 / Production host: Escherichia coli (E. coli) / References: UniProt: B7LEG6, UniProt: P0A6S5*PLUS |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60.35 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.15 M potassium thiocyanate, 20 % PEG 550 MME, 0.1 M sodium cacodylate pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92819 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Feb 22, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92819 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→30 Å / Num. obs: 15037 / % possible obs: 99.5 % / Redundancy: 12.8 % / CC1/2: 0.999 / Rmerge(I) obs: 0.082 / Rpim(I) all: 0.024 / Net I/σ(I): 22.6 |
Reflection shell | Resolution: 2.6→2.74 Å / Redundancy: 13.4 % / Rmerge(I) obs: 1.702 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 2155 / CC1/2: 0.821 / Rpim(I) all: 0.477 / % possible all: 99.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2VH1 Resolution: 2.6→29.994 Å / SU ML: 0.42 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 30.93
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→29.994 Å
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Refine LS restraints |
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LS refinement shell |
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