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Yorodumi- PDB-6h8o: Crystal structure of the Master-Rep protein nuclease domain from ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6h8o | |||||||||
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Title | Crystal structure of the Master-Rep protein nuclease domain from the Faba Bean Necrotic Yellows Virus | |||||||||
Components | Replication-associated protein | |||||||||
Keywords | REPLICATION / endonuclease / Faba Bean Necrotic Yellows Virus / Rep protein / DNA replication | |||||||||
Function / homology | Function and homology information : / endodeoxyribonuclease activity, producing 5'-phosphomonoesters / Hydrolases; Acting on ester bonds; Endodeoxyribonucleases producing 5'-phosphomonoesters / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / nucleotidyltransferase activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / DNA replication / RNA helicase activity / host cell nucleus / ATP hydrolysis activity ...: / endodeoxyribonuclease activity, producing 5'-phosphomonoesters / Hydrolases; Acting on ester bonds; Endodeoxyribonucleases producing 5'-phosphomonoesters / Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides / nucleotidyltransferase activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / DNA replication / RNA helicase activity / host cell nucleus / ATP hydrolysis activity / DNA binding / RNA binding / ATP binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Faba bean necrotic yellows virus | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.15 Å | |||||||||
Authors | Moncalian, G. / Gonzalez-Montes, L. | |||||||||
Funding support | Spain, 1items
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Citation | Journal: To Be Published Title: Crystal structure of the Master-Rep protein nuclease domain from the Faba Bean Necrotic Yellows Virus Authors: Moncalian, G. / Gonzalez-Montes, L. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6h8o.cif.gz | 86.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6h8o.ent.gz | 70.1 KB | Display | PDB format |
PDBx/mmJSON format | 6h8o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h8/6h8o ftp://data.pdbj.org/pub/pdb/validation_reports/h8/6h8o | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 10954.520 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Faba bean necrotic yellows virus / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A2D0XNJ4, UniProt: O39828*PLUS #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.9 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop Details: 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Sodium citrate tribasis dihydrate pH5.6, 2.0 M Ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALBA / Beamline: XALOC / Wavelength: 0.99989 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jun 3, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99989 Å / Relative weight: 1 |
Reflection | Resolution: 1.15→47.115 Å / Num. obs: 81485 / % possible obs: 99.4 % / Redundancy: 15.4 % / Biso Wilson estimate: 11.36 Å2 / CC1/2: 0.997 / Net I/σ(I): 19.5 |
Reflection shell | Resolution: 1.15→1.21 Å / Redundancy: 6.3 % / Mean I/σ(I) obs: 5.6 / Num. unique obs: 11361 / CC1/2: 0.944 / % possible all: 95.6 |
-Processing
Software |
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Refinement | Method to determine structure: SAD Starting model: I3C Resolution: 1.15→47.115 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.15→47.115 Å
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LS refinement shell | Resolution: 1.15→1.191 Å
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