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Open data
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Basic information
Entry | Database: PDB / ID: 1bxm | |||||||||
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Title | ENGINEERED BETA-CRYPTOGEIN COMPLEXED WITH ERGOSTEROL | |||||||||
![]() | BETA-CRYPTOGEIN | |||||||||
![]() | FUNGAL TOXIC ELICITOR / FUNGAL TOXIC ELICITOR MUTANT / ELICITIN / PHYTOPHTHORA / STEROL / PLANT PATHOGEN | |||||||||
Function / homology | ![]() symbiont-mediated perturbation of host defense-related programmed cell death / symbiont-mediated killing of host cell / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Boissy, G. / Brunie, S. | |||||||||
![]() | ![]() Title: The 2.1 A structure of an elicitin-ergosterol complex: a recent addition to the Sterol Carrier Protein family. Authors: Boissy, G. / O'Donohue, M. / Gaudemer, O. / Perez, V. / Pernollet, J.C. / Brunie, S. #1: ![]() Title: Crystal Structure of a Fungal Elicitor Secreted by Phytophthora Cryptogea, a Member of a Novel Class of Plant Necrotic Proteins Authors: Boissy, G. / De La Fortelle, E. / Kahn, R. / Huet, J.C. / Bricogne, G. / Pernollet, J.C. / Brunie, S. #2: ![]() Title: Overexpression in Pichia Pastoris and Crystallization of an Elicitor Protein Secreted by the Phytopathogenic Fungus, Phytophthora Cryptogea Authors: O'Donohue, M.J. / Boissy, G. / Huet, J.C. / Nespoulous, C. / Brunie, S. / Pernollet, J.C. #3: ![]() Title: Crystallization and Preliminary X-Ray Diffraction Studies of Beta-Cryptogein, a Toxic Elicitin Secreted by the Phytopathogenic Fungus Phytophthora Cryptogea Authors: Guilloteau, J.P. / Nespoulous, C. / Huet, J.C. / Beauvais, F. / Pernollet, J.C. / Brunie, S. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 28.7 KB | Display | ![]() |
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PDB format | ![]() | 21.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.9 KB | Display | ![]() |
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Full document | ![]() | 440.8 KB | Display | |
Data in XML | ![]() | 3.6 KB | Display | |
Data in CIF | ![]() | 5.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 10492.951 Da / Num. of mol.: 1 / Mutation: T1G, A2T, K13H Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Description: N-TERMINAL ARGININE INCORPORATION NON-BIOLOGICAL SEQUENCE Cell line: PICHIA PASTORIS / Gene: CRY (MUTATED LYS 13 HIS) / Plasmid: PHILS1 / Gene (production host): CRY (MUTATED LYS 13 HIS) / Production host: ![]() |
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#2: Chemical | ChemComp-ERG / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 47 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.5 / Details: pH 4.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 1, 1996 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→10 Å / Num. obs: 5370 / % possible obs: 96.1 % / Observed criterion σ(I): 2 / Redundancy: 6.1 % / Biso Wilson estimate: 20.9 Å2 / Rsym value: 0.046 |
Reflection | *PLUS Num. measured all: 33143 / Rmerge(I) obs: 0.046 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: THE REFINED 2.2 ANGSTROMS RESOLUTION STRUCTURE OF THE WILD-TYPE BETA-CRYPTOGEIN (1BEO). THE ERGOSTEROL MOLECULE WAS CONSTRUCTED USING, AS A TEMPLATE, THE COORDINATES OF THE ...Starting model: THE REFINED 2.2 ANGSTROMS RESOLUTION STRUCTURE OF THE WILD-TYPE BETA-CRYPTOGEIN (1BEO). THE ERGOSTEROL MOLECULE WAS CONSTRUCTED USING, AS A TEMPLATE, THE COORDINATES OF THE CHOLESTERYL LINOLEATE MOLECULE COMPLEXED TO THE CHOLESTEROL ESTERASE (1CLE). Resolution: 2.15→8 Å / Rfactor Rfree error: 0.015 / Data cutoff high absF: 100000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 3 / Details: RESOLUTION-DEPENDENT WEIGHTING SCHEME USED.
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Displacement parameters | Biso mean: 18.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.15→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.15→2.28 Å / Rfactor Rfree error: 0.052 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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