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Open data
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Basic information
Entry | Database: PDB / ID: 6gb1 | ||||||
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Title | Crystal structure of the GLP1 receptor ECD with Peptide 11 | ||||||
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![]() | PEPTIDE BINDING PROTEIN / Glucagon-like Peptide 1 Protein-Peptide complex Incretins Diabetes drug discovery | ||||||
Function / homology | ![]() glucagon-like peptide 1 receptor activity / glucagon receptor activity / hormone secretion / positive regulation of blood pressure / post-translational protein targeting to membrane, translocation / response to psychosocial stress / regulation of heart contraction / peptide hormone binding / activation of adenylate cyclase activity / negative regulation of blood pressure ...glucagon-like peptide 1 receptor activity / glucagon receptor activity / hormone secretion / positive regulation of blood pressure / post-translational protein targeting to membrane, translocation / response to psychosocial stress / regulation of heart contraction / peptide hormone binding / activation of adenylate cyclase activity / negative regulation of blood pressure / cAMP-mediated signaling / adenylate cyclase-activating G protein-coupled receptor signaling pathway / Glucagon-type ligand receptors / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / transmembrane signaling receptor activity / positive regulation of cytosolic calcium ion concentration / G alpha (s) signalling events / learning or memory / cell surface receptor signaling pathway / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schreuder, H.A. / Liesum, A. | ||||||
![]() | ![]() Title: Dual Glucagon-like Peptide 1 (GLP-1)/Glucagon Receptor Agonists Specifically Optimized for Multidose Formulations. Authors: Evers, A. / Bossart, M. / Pfeiffer-Marek, S. / Elvert, R. / Schreuder, H. / Kurz, M. / Stengelin, S. / Lorenz, M. / Herling, A. / Konkar, A. / Lukasczyk, U. / Pfenninger, A. / Lorenz, K. / ...Authors: Evers, A. / Bossart, M. / Pfeiffer-Marek, S. / Elvert, R. / Schreuder, H. / Kurz, M. / Stengelin, S. / Lorenz, M. / Herling, A. / Konkar, A. / Lukasczyk, U. / Pfenninger, A. / Lorenz, K. / Haack, T. / Kadereit, D. / Wagner, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 44.3 KB | Display | ![]() |
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PDB format | ![]() | 30.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.9 KB | Display | ![]() |
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Full document | ![]() | 462.8 KB | Display | |
Data in XML | ![]() | 8.3 KB | Display | |
Data in CIF | ![]() | 10.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6gdzC ![]() 6ge2C ![]() 3c59S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 14833.366 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Protein/peptide | Mass: 3243.687 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: N-terminally truncated Dual Glucagon-like Peptide 1 (GLP-1)/Glucagon Receptor Agonist Source: (synth.) synthetic construct (others) | ||||
#3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.42 Å3/Da / Density % sol: 64.02 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: A 6-fold excess of peptide 11 was dissolved in a solution of 12 mg/ml GLP1R-ECD in 10 mM Tris buffer pH 7.5, 100 mM NaSulfate and 2% glycerol. 100 nl of this protein solution plus 100 nl ...Details: A 6-fold excess of peptide 11 was dissolved in a solution of 12 mg/ml GLP1R-ECD in 10 mM Tris buffer pH 7.5, 100 mM NaSulfate and 2% glycerol. 100 nl of this protein solution plus 100 nl reservoir solution were equilibrated against reservoir solution consisting of 10 mM CoCl2, 9.4 % (v/v) 1,6-hexanediol and 100 mM NaAcetate, pH 4.8. Crystals appeared after about one week. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 9, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99992 Å / Relative weight: 1 |
Reflection | Resolution: 2.73→51.57 Å / Num. obs: 6336 / % possible obs: 100 % / Redundancy: 12 % / Biso Wilson estimate: 95.53 Å2 / Rmerge(I) obs: 0.075 / Rsym value: 0.075 / Net I/σ(I): 22 |
Reflection shell | Resolution: 2.73→2.87 Å / Redundancy: 12.8 % / Rmerge(I) obs: 1.096 / Mean I/σ(I) obs: 2.3 / Rsym value: 1.096 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3c59 Resolution: 2.73→51.57 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.907 / SU R Cruickshank DPI: 0.478 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.615 / SU Rfree Blow DPI: 0.32 / SU Rfree Cruickshank DPI: 0.308
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Displacement parameters | Biso mean: 83.65 Å2
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Refine analyze | Luzzati coordinate error obs: 0.35 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.73→51.57 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.73→3.05 Å / Total num. of bins used: 5
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