pyrimidine nucleoside catabolic process / beta-ureidopropionase / CMP catabolic process / UMP catabolic process / dCMP catabolic process / beta-alanine biosynthetic process via 3-ureidopropionate / dUMP catabolic process / beta-ureidopropionase activity / Pyrimidine catabolism / liver development ...pyrimidine nucleoside catabolic process / beta-ureidopropionase / CMP catabolic process / UMP catabolic process / dCMP catabolic process / beta-alanine biosynthetic process via 3-ureidopropionate / dUMP catabolic process / beta-ureidopropionase activity / Pyrimidine catabolism / liver development / protein homooligomerization / protein homotetramerization / in utero embryonic development / protein homodimerization activity / extracellular exosome / cytosol Similarity search - Function
Evidence: gel filtration, the native enzyme occurs in several oligomeric states, the T299C variant exclusively as homodimer, as determined by gel filtration, native PAGE, static light scattering, and ...Evidence: gel filtration, the native enzyme occurs in several oligomeric states, the T299C variant exclusively as homodimer, as determined by gel filtration, native PAGE, static light scattering, and the crystal structure
Mass: 43883.691 Da / Num. of mol.: 1 / Mutation: T299C Source method: isolated from a genetically manipulated source Details: the first 6 amino acids are a remainder of a cleaved His-tag C233 is oxidized to cysteine sulfinic acid T299 is mutated to cysteine Source: (gene. exp.) Homo sapiens (human) / Gene: UPB1, BUP1 / Plasmid: pET151/D-TOPO / Production host: Escherichia coli BL21 (bacteria) / Variant (production host): AI / References: UniProt: Q9UBR1, beta-ureidopropionase
Resolution: 2.08→28.1 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.948 / SU B: 6.925 / SU ML: 0.095 / Cross valid method: THROUGHOUT / ESU R: 0.159 / ESU R Free: 0.137 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.1991
1453
5.1 %
RANDOM
Rwork
0.17539
-
-
-
obs
0.17656
26915
99.93 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å