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- PDB-6ftp: Crystal form 1 of Alpha1-antichymotrypsin variant DBS-II-allo: an... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ftp | ||||||
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Title | Crystal form 1 of Alpha1-antichymotrypsin variant DBS-II-allo: an allosterically modulated drug-binding serpin for doxorubicin | ||||||
![]() | (Alpha-1-antichymotrypsin) x 2 | ||||||
![]() | TRANSPORT PROTEIN / Serpin / alpha1-Antichymotrypsin / Doxorubicin-binding protein / allosterically triggered drug release | ||||||
Function / homology | ![]() maintenance of gastrointestinal epithelium / regulation of lipid metabolic process / platelet alpha granule lumen / acute-phase response / serine-type endopeptidase inhibitor activity / azurophil granule lumen / Platelet degranulation / secretory granule lumen / collagen-containing extracellular matrix / blood microparticle ...maintenance of gastrointestinal epithelium / regulation of lipid metabolic process / platelet alpha granule lumen / acute-phase response / serine-type endopeptidase inhibitor activity / azurophil granule lumen / Platelet degranulation / secretory granule lumen / collagen-containing extracellular matrix / blood microparticle / inflammatory response / Neutrophil degranulation / DNA binding / extracellular space / extracellular exosome / extracellular region / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schmidt, K. / Muller, Y.A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Design of an allosterically modulated doxycycline and doxorubicin drug-binding protein. Authors: Schmidt, K. / Gardill, B.R. / Kern, A. / Kirchweger, P. / Borsch, M. / Muller, Y.A. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.7 KB | Display | ![]() |
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PDB format | ![]() | 74.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5om2C ![]() 5om3C ![]() 5om5C ![]() 5om6C ![]() 5om7C ![]() 5om8SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 41986.516 Da / Num. of mol.: 1 Mutation: L24R W194F W215Y E242Q K244N L269S P270Q K274S W276F R277F D278E A349R V355L K356E I357V T358L L359F L360Q Source method: isolated from a genetically manipulated source Details: All N-terminal residues that are present in the sample sequence but not in the PDB file could not be modelled due to missing electron density. The protein is splitted into chain A and chain ...Details: All N-terminal residues that are present in the sample sequence but not in the PDB file could not be modelled due to missing electron density. The protein is splitted into chain A and chain B, as it belongs to the serine proteinase inhibitor (serpin) family and is proteolytically cleaved C-terminal of Gln360. The residues Gln360 and Arg376, which is the first residue visible in the electron density, are 70 angstrom apart because of the serpin-typical conformational change upon proteolytical cleavage. The sequence following the P1-P1' scissile bond, namely residues GPL..KQA, are part of chain B. Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Protein/peptide | Mass: 4720.580 Da / Num. of mol.: 1 / Mutation: S361G A362P P382D T383N D384F Q386W N387S Source method: isolated from a genetically manipulated source Details: The protein is splitted into chain A and chain B, as it belongs to the serine proteinase inhibitor (serpin) family and is proteolytically cleaved C-terminal of Gln360. The residues Gln360 ...Details: The protein is splitted into chain A and chain B, as it belongs to the serine proteinase inhibitor (serpin) family and is proteolytically cleaved C-terminal of Gln360. The residues Gln360 and Arg376, which is the first residue visible in the electron density, are 70 angstrom apart because of the serpin-typical conformational change upon proteolytical cleavage. The sequence following the P1-P1' scissile bond, namely residues GPL..KQA, are part of chain B. The residues 361-366 (GPLVET) are missing in the PDB file because of missing electron density. Source: (gene. exp.) ![]() ![]() ![]() | ||||
#3: Chemical | #4: Chemical | ChemComp-DM2 / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.44 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M sodium thiocyanate, 20 % w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: May 12, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9184 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→42.12 Å / Num. obs: 37572 / % possible obs: 99.9 % / Redundancy: 9.9 % / CC1/2: 1 / Rrim(I) all: 0.061 / Rsym value: 0.058 / Net I/σ(I): 25.31 |
Reflection shell | Resolution: 1.8→1.91 Å / Redundancy: 10.1 % / Mean I/σ(I) obs: 2.76 / Num. unique obs: 5991 / CC1/2: 0.854 / Rrim(I) all: 0.878 / Rsym value: 0.834 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5OM8 Resolution: 1.8→42.12 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 23.34
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→42.12 Å
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Refine LS restraints |
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LS refinement shell |
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