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- PDB-6fsq: Structure of A3_bGFPD, an artificial bi-domain protein based on t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fsq | ||||||
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Title | Structure of A3_bGFPD, an artificial bi-domain protein based on two different alphaRep domains : A3 and a GFP binding domain (bGFPD) | ||||||
![]() | alphaRep A3_bGFPD | ||||||
![]() | BIOSYNTHETIC PROTEIN / alphaRep / artificial protein / chimera / bidomain | ||||||
Function / homology | MALONATE ION![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li de la Sierra-Gallay, I. / Leger, C. | ||||||
![]() | ![]() Title: Ligand-induced conformational switch in an artificial bidomain protein scaffold. Authors: Leger, C. / Di Meo, T. / Aumont-Nicaise, M. / Velours, C. / Durand, D. / Li de la Sierra-Gallay, I. / van Tilbeurgh, H. / Hildebrandt, N. / Desmadril, M. / Urvoas, A. / Valerio-Lepiniec, M. / Minard, P. #1: ![]() Title: Bi-domain artificial protein alphaRep display inducible cooperativity Authors: Leger, C. / Gallay, I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83 KB | Display | ![]() |
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PDB format | ![]() | 62.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 440.2 KB | Display | ![]() |
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Full document | ![]() | 443.3 KB | Display | |
Data in XML | ![]() | 14.2 KB | Display | |
Data in CIF | ![]() | 18.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ft5C ![]() 6hwpC ![]() 3ltjS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 44460.203 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-MLI / |
#3: Chemical | ChemComp-NA / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.88 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 / Details: 1.6M Tri-sodium citrate |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 11, 2015 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.79→47.869 Å / Num. obs: 10938 / % possible obs: 99.6 % / Redundancy: 12.759 % / Biso Wilson estimate: 48.68 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.206 / Rrim(I) all: 0.214 / Χ2: 0.898 / Net I/σ(I): 12.95 / Num. measured all: 139553 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3LTJ Resolution: 2.79→44.51 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.889 / SU B: 20.923 / SU ML: 0.38 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.401 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 129.75 Å2 / Biso mean: 61.873 Å2 / Biso min: 31.89 Å2
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Refinement step | Cycle: final / Resolution: 2.79→44.51 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.792→2.865 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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