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- PDB-1bx2: CRYSTAL STRUCTURE OF HLA-DR2 (DRA*0101,DRB1*1501) COMPLEXED WITH ... -
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Basic information
Entry | Database: PDB / ID: 1bx2 | ||||||
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Title | CRYSTAL STRUCTURE OF HLA-DR2 (DRA*0101,DRB1*1501) COMPLEXED WITH A PEPTIDE FROM HUMAN MYELIN BASIC PROTEIN | ||||||
![]() | (PROTEIN (HLA-DR2)) x 3 | ||||||
![]() | IMMUNE SYSTEM / HLA-DR2 / MYELIN BASIC PROTEIN / MULTIPLE SCLEROSIS / AUTOIMMUNITY | ||||||
Function / homology | ![]() compact myelin / structural constituent of myelin sheath / positive regulation of metalloendopeptidase activity / internode region of axon / axon ensheathment / negative regulation of heterotypic cell-cell adhesion / regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II ...compact myelin / structural constituent of myelin sheath / positive regulation of metalloendopeptidase activity / internode region of axon / axon ensheathment / negative regulation of heterotypic cell-cell adhesion / regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / positive regulation of T cell mediated immune response to tumor cell / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / MHC class II receptor activity / EGR2 and SOX10-mediated initiation of Schwann cell myelination / positive regulation of CD4-positive, alpha-beta T cell activation / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / membrane organization / positive regulation of memory T cell differentiation / positive regulation of monocyte differentiation / CD4 receptor binding / inflammatory response to antigenic stimulus / positive regulation of kinase activity / positive regulation of chemokine (C-X-C motif) ligand 2 production / transport vesicle membrane / intermediate filament / polysaccharide binding / T-helper 1 type immune response / maintenance of blood-brain barrier / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / positive regulation of insulin secretion involved in cellular response to glucose stimulus / humoral immune response / macrophage differentiation / negative regulation of type II interferon production / Generation of second messenger molecules / immunological synapse / PD-1 signaling / epidermis development / T cell receptor binding / detection of bacterium / negative regulation of T cell proliferation / negative regulation of inflammatory response to antigenic stimulus / substantia nigra development / MHC class II antigen presentation / myelination / trans-Golgi network membrane / central nervous system development / cell periphery / lumenal side of endoplasmic reticulum membrane / protein tetramerization / sensory perception of sound / clathrin-coated endocytic vesicle membrane / ER to Golgi transport vesicle membrane / structural constituent of cytoskeleton / response to toxic substance / cognition / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / positive regulation of T cell mediated cytotoxicity / peptide antigen binding / antigen processing and presentation of exogenous peptide antigen via MHC class II / endocytic vesicle membrane / positive regulation of interleukin-6 production / Interferon gamma signaling / positive regulation of immune response / MAPK cascade / Downstream TCR signaling / positive regulation of T cell activation / myelin sheath / MHC class II protein complex binding / late endosome membrane / T cell receptor signaling pathway / early endosome membrane / chemical synaptic transmission / positive regulation of canonical NF-kappaB signal transduction / adaptive immune response / protease binding / positive regulation of MAPK cascade / positive regulation of viral entry into host cell / positive regulation of ERK1 and ERK2 cascade / lysosome / calmodulin binding / positive regulation of protein phosphorylation / immune response / lysosomal membrane / external side of plasma membrane / Golgi membrane / neuronal cell body / lipid binding / synapse / positive regulation of DNA-templated transcription / cell surface / signal transduction / protein-containing complex / extracellular space / extracellular exosome / membrane / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Smith, K.J. / Pyrdol, J. / Gauthier, L. / Wiley, D.C. / Wucherpfennig, K. | ||||||
![]() | ![]() Title: Crystal structure of HLA-DR2 (DRA*0101, DRB1*1501) complexed with a peptide from human myelin basic protein. Authors: Smith, K.J. / Pyrdol, J. / Gauthier, L. / Wiley, D.C. / Wucherpfennig, K.W. #1: ![]() Title: Molecular Mimicry in T Cell Mediated Autoimmunity:Viral Peptides Activate Human T Cell Clones Specific for Myelin Basic Protein Authors: Wucherpfennig, K. / Strominger, J.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 166.6 KB | Display | ![]() |
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PDB format | ![]() | 132.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 425.8 KB | Display | ![]() |
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Full document | ![]() | 448 KB | Display | |
Data in XML | ![]() | 18.7 KB | Display | |
Data in CIF | ![]() | 27.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1dlhS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20971.670 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR DOMAINS ALPHA 1, ALPHA 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein | Mass: 22316.930 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR DOMAINS BETA 1, BETA 2 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Protein/peptide | Mass: 1800.086 Da / Num. of mol.: 2 / Fragment: PEPTIDE FROM HUMAN MYELIN BASIC PROTEIN / Source method: isolated from a natural source Details: MYELIN BASIC PROTEIN PEPTIDE WAS COVALENTLY LINKED TO THE N-TERMINUS OF THE HLA-DR2 BETA CHAIN Source: (natural) ![]() #4: Sugar | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 62 % | |||||||||||||||||||||||||
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Crystal grow | pH: 3.5 / Details: 15-18% PEG6000 100MM GLYCINE PH3.5 | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7.5 / Method: unknown | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC / Detector: CCD / Date: Mar 18, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→20 Å / Num. obs: 42589 / % possible obs: 88.3 % / Observed criterion σ(I): -3 / Redundancy: 6 % / Biso Wilson estimate: 37.6 Å2 / Rmerge(I) obs: 0.088 / Net I/σ(I): 23 |
Reflection shell | Resolution: 2.49→2.6 Å / Rmerge(I) obs: 0.27 / Mean I/σ(I) obs: 3.9 / % possible all: 83.1 |
Reflection | *PLUS Num. measured all: 263333 |
Reflection shell | *PLUS % possible obs: 83.1 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1DLH Resolution: 2.6→20 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 1000000 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 35.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.6→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.72 Å / Rfactor Rfree error: 0.025 / Total num. of bins used: 8
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Xplor file |
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