negative regulation of protein deubiquitination / ISG15 transferase activity / ISG15-protein conjugation / regulation of viral entry into host cell / protein K27-linked ubiquitination / suppression of viral release by host / Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins / Aberrant regulation of mitotic exit in cancer due to RB1 defects ...negative regulation of protein deubiquitination / ISG15 transferase activity / ISG15-protein conjugation / regulation of viral entry into host cell / protein K27-linked ubiquitination / suppression of viral release by host / Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins / Aberrant regulation of mitotic exit in cancer due to RB1 defects / (E3-independent) E2 ubiquitin-conjugating enzyme / regulation of type I interferon production / cellular response to chemical stress / Phosphorylation of the APC/C / negative regulation of viral transcription / protein K6-linked ubiquitination / STING mediated induction of host immune responses / stress granule disassembly / E2 ubiquitin-conjugating enzyme / negative regulation of NF-kappaB transcription factor activity / pyroptotic inflammatory response / response to type II interferon / protein monoubiquitination / ubiquitin conjugating enzyme activity / Regulation of APC/C activators between G1/S and early anaphase / protein K63-linked ubiquitination / Transcriptional Regulation by VENTX / autophagosome / proteasomal protein catabolic process / protein autoubiquitination / protein K48-linked ubiquitination / positive regulation of autophagy / positive regulation of cell cycle / ubiquitin ligase complex / APC/C:Cdc20 mediated degradation of Cyclin B / APC-Cdc20 mediated degradation of Nek2A / Regulation of innate immune responses to cytosolic DNA / negative regulation of innate immune response / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin / Assembly of the pre-replicative complex / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / P-body / protein destabilization / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / RING-type E3 ubiquitin transferase / CDK-mediated phosphorylation and removal of Cdc6 / ISG15 antiviral mechanism / positive regulation of DNA-binding transcription factor activity / cytoplasmic stress granule / protein polyubiquitination / Separation of Sister Chromatids / ubiquitin-protein transferase activity / regulation of protein localization / Interferon gamma signaling / KEAP1-NFE2L2 pathway / ubiquitin protein ligase activity / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of protein binding / E3 ubiquitin ligases ubiquitinate target proteins / Senescence-Associated Secretory Phenotype (SASP) / cytoplasmic vesicle / ubiquitin-dependent protein catabolic process / positive regulation of canonical NF-kappaB signal transduction / transcription coactivator activity / positive regulation of viral entry into host cell / protein ubiquitination / ribonucleoprotein complex / cell cycle / innate immune response / protein kinase binding / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / zinc ion binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function
Method to determine structure: MAD / Resolution: 2.82→47.9 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.887 / SU B: 25.399 / SU ML: 0.455 / Cross valid method: THROUGHOUT / ESU R Free: 0.453 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.29407
2549
5 %
RANDOM
Rwork
0.25283
-
-
-
obs
0.25492
48491
91.19 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å