A: Synaptonemal complex protein 1 hetero molecules
A: Synaptonemal complex protein 1 hetero molecules
A: Synaptonemal complex protein 1 hetero molecules
A: Synaptonemal complex protein 1 hetero molecules
defined by author&software
Evidence: light scattering, Head-to-head assembly occurs in the context of the wider molecule through cooperativity; confirmed through MALS and SAXS with point mutagenesis.
Mass: 18.015 Da / Num. of mol.: 32 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.55 Å3/Da / Density % sol: 51.75 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.2 Details: 140 mM NaCl, 70 mM Na/K phosphate pH 6.2, 35% (v/v) PEG200; soaked in 40% (v/v) PEG200 and 100 mM NaI
Method to determine structure: SAD / Resolution: 1.91→41.44 Å / SU ML: 0.27 / Cross valid method: FREE R-VALUE / σ(F): 2.02 / Phase error: 25.34 Details: Refined against data corrected for anisotropy (FP_ISOB/SIGFP_ISOB) using the UCLA diffraction anisotropy server.
Rfactor
Num. reflection
% reflection
Rfree
0.2392
324
4.8 %
Rwork
0.2272
-
-
obs
0.2278
6754
87.97 %
Solvent computation
Shrinkage radii: 0.7 Å / VDW probe radii: 1 Å
Displacement parameters
Biso mean: 58.4 Å2
Refinement step
Cycle: LAST / Resolution: 1.91→41.44 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
624
0
12
32
668
Refine LS restraints
Refine-ID
Type
Dev ideal
Number
X-RAY DIFFRACTION
f_bond_d
0.009
648
X-RAY DIFFRACTION
f_angle_d
1.022
857
X-RAY DIFFRACTION
f_dihedral_angle_d
10.579
273
X-RAY DIFFRACTION
f_chiral_restr
0.053
92
X-RAY DIFFRACTION
f_plane_restr
0.004
108
LS refinement shell
Resolution (Å)
Rfactor Rfree
Num. reflection Rfree
Rfactor Rwork
Num. reflection Rwork
Refine-ID
% reflection obs (%)
1.9118-2.0316
0.3309
28
0.3174
437
X-RAY DIFFRACTION
38
2.0316-2.1884
0.2647
61
0.2753
1078
X-RAY DIFFRACTION
91
2.1884-2.4086
0.2572
56
0.2278
1188
X-RAY DIFFRACTION
100
2.4086-2.7571
0.225
52
0.2613
1206
X-RAY DIFFRACTION
100
2.7571-3.4734
0.264
64
0.2476
1232
X-RAY DIFFRACTION
100
3.4734-41.4522
0.2158
63
0.1945
1289
X-RAY DIFFRACTION
98
+
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