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- PDB-6e9t: DHF58 filament -

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Basic information

Entry
Database: PDB / ID: 6e9t
TitleDHF58 filament
ComponentsDHF58 filament
KeywordsPROTEIN FIBRIL / Protein design / filament
Specimen sourcesynthetic construct (others)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / 5.4 Å resolution
AuthorsLynch, E.M. / Shen, H. / Fallas, J.A. / Kollman, J.M. / Baker, D.
CitationJournal: Science / Year: 2018
Title: De novo design of self-assembling helical protein filaments.
Authors: Hao Shen / Jorge A Fallas / Eric Lynch / William Sheffler / Bradley Parry / Nicholas Jannetty / Justin Decarreau / Michael Wagenbach / Juan Jesus Vicente / Jiajun Chen / Lei Wang / Quinton Dowling / Gustav Oberdorfer / Lance Stewart / Linda Wordeman / James De Yoreo / Christine Jacobs-Wagner / Justin Kollman / David Baker
Abstract: We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale ...We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale filaments with a wide range of geometries in vivo and in vitro. Cryo-electron microscopy structures of six designs are close to the computational design models. The filament building blocks are idealized repeat proteins, and thus the diameter of the filaments can be systematically tuned by varying the number of repeat units. The assembly and disassembly of the filaments can be controlled by engineered anchor and capping units built from monomers lacking one of the interaction surfaces. The ability to generate dynamic, highly ordered structures that span micrometers from protein monomers opens up possibilities for the fabrication of new multiscale metamaterials.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Aug 1, 2018 / Release: Nov 21, 2018

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Assembly

Deposited unit
B: DHF58 filament
A: DHF58 filament
N: DHF58 filament
C: DHF58 filament
O: DHF58 filament
D: DHF58 filament
P: DHF58 filament
E: DHF58 filament
Q: DHF58 filament
F: DHF58 filament
R: DHF58 filament
G: DHF58 filament
S: DHF58 filament
H: DHF58 filament
T: DHF58 filament
I: DHF58 filament
U: DHF58 filament
J: DHF58 filament
V: DHF58 filament
K: DHF58 filament
W: DHF58 filament
L: DHF58 filament
X: DHF58 filament
M: DHF58 filament


Theoretical massNumber of molelcules
Total (without water)606,56124
Polyers606,56124
Non-polymers00
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (Å2)40130
ΔGint (kcal/M)-84
Surface area (Å2)247300
Helical symmetryCircular symmetry: 2 / Dyad axis: no / N subunits divisor: 1 / Number of operations: 12 / Rise per n subunits: 9.10835 Å / Rotation per n subunits: 40.93287 deg.

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Components

#1: Protein/peptide ...
DHF58 filament


Mass: 25273.379 Da / Num. of mol.: 24 / Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / Reconstruction method: helical reconstruction

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Sample preparation

ComponentName: DHF58 filament / Type: COMPLEX / Entity ID: 1 / Source: RECOMBINANT
Source (natural)Organism: synthetic construct (others)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: unspecified
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 45 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

EM software
IDNameCategory
2Leginonimage acquisition
10SPIDERinitial Euler assignment
11SPIDERfinal Euler assignment
13SPIDER3D reconstruction
CTF correctionType: PHASE FLIPPING ONLY
Helical symmertyAngular rotation/subunit: 40.93287 deg. / Axial rise/subunit: 9.10835 Å / Axial symmetry: C2
3D reconstructionResolution: 5.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 76994 / Symmetry type: HELICAL
Atomic model buildingRef protocol: RIGID BODY FIT

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