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TitleDe novo design of self-assembling helical protein filaments.
Journal, issue, pagesScience, Vol. 362, Issue 6415, Page 705-709, Year 2018
Publish dateNov 9, 2018
AuthorsHao Shen / Jorge A Fallas / Eric Lynch / William Sheffler / Bradley Parry / Nicholas Jannetty / Justin Decarreau / Michael Wagenbach / Juan Jesus Vicente / Jiajun Chen / Lei Wang / Quinton Dowling / Gustav Oberdorfer / Lance Stewart / Linda Wordeman / James De Yoreo / Christine Jacobs-Wagner / Justin Kollman / David Baker /
PubMed AbstractWe describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale ...We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale filaments with a wide range of geometries in vivo and in vitro. Cryo-electron microscopy structures of six designs are close to the computational design models. The filament building blocks are idealized repeat proteins, and thus the diameter of the filaments can be systematically tuned by varying the number of repeat units. The assembly and disassembly of the filaments can be controlled by engineered anchor and capping units built from monomers lacking one of the interaction surfaces. The ability to generate dynamic, highly ordered structures that span micrometers from protein monomers opens up possibilities for the fabrication of new multiscale metamaterials.
External linksScience / PubMed:30409885 / PubMed Central
MethodsEM (helical sym.)
Resolution3.4 - 7.8 Å
Structure data

9016

6e9r

EMDB-9016, PDB-6e9r:
DHF46 filament
Method: EM (helical sym.) / Resolution: 5.9 Å

9017

6e9t

EMDB-9017, PDB-6e9t:
DHF58 filament
Method: EM (helical sym.) / Resolution: 5.4 Å

9018

6e9v

EMDB-9018, PDB-6e9v:
DHF79 filament
Method: EM (helical sym.) / Resolution: 6.9 Å

9019

6e9x

EMDB-9019, PDB-6e9x:
DHF91 filament
Method: EM (helical sym.) / Resolution: 7.8 Å

9020

6e9y

EMDB-9020, PDB-6e9y:
DHF38 filament
Method: EM (helical sym.) / Resolution: 4.3 Å

9021

6e9z

EMDB-9021, PDB-6e9z:
DHF119 filament
Method: EM (helical sym.) / Resolution: 3.4 Å

Source
  • synthetic construct (others)
KeywordsPROTEIN FIBRIL / Protein design / filament

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