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- PDB-6e66: Crystal structure of bacterial N-acetylglucosamine transferase NleB -
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Open data
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Basic information
Entry | Database: PDB / ID: 6.0E+66 | ||||||
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Title | Crystal structure of bacterial N-acetylglucosamine transferase NleB | ||||||
![]() | NleB | ||||||
![]() | TRANSFERASE / NleB / Bacterial effector / Arginine GlcNAcylation | ||||||
Function / homology | ![]() protein-arginine N-acetylglucosaminyltransferase activity / symbiont-mediated suppression of host defense-related programmed cell death / symbiont-mediated suppression of host NF-kappaB cascade / Transferases; Glycosyltransferases; Hexosyltransferases / manganese ion binding / toxin activity / host cell cytoplasm / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yao, Q. / Zheng, Y.Q. / Shao, F. | ||||||
![]() | ![]() Title: Structural and Functional Insights into Host Death Domains Inactivation by the Bacterial Arginine GlcNAcyltransferase Effector. Authors: Ding, J. / Pan, X. / Du, L. / Yao, Q. / Xue, J. / Yao, H. / Wang, D.C. / Li, S. / Shao, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.9 KB | Display | ![]() |
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PDB format | ![]() | 53.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.9 KB | Display | ![]() |
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Full document | ![]() | 442.3 KB | Display | |
Data in XML | ![]() | 13 KB | Display | |
Data in CIF | ![]() | 17.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38175.699 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-EDO / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.82 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.2 ammonium sulfate, 0.1 M sodium cacodylate trihydrate at pH 6.5 and 30% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 77.2 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 12, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→20 Å / Num. obs: 19964 / % possible obs: 97.94 % / Observed criterion σ(I): 3 / Redundancy: 6.5 % / Rmerge(I) obs: 0.214 / Net I/σ(I): 21.4 |
Reflection shell | Resolution: 2.1→2.14 Å / Rmerge(I) obs: 0.439 / Num. unique obs: 1873 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→19.744 Å
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Refine LS restraints |
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LS refinement shell |
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