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Yorodumi- PDB-6dj3: Crystal structure of CNNM2 cyclic nucleotide-binding homology domain -
+Open data
-Basic information
Entry | Database: PDB / ID: 6dj3 | ||||||
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Title | Crystal structure of CNNM2 cyclic nucleotide-binding homology domain | ||||||
Components | Metal transporter CNNM2 | ||||||
Keywords | TRANSPORT PROTEIN / CNNM2 / cyclic nucleotide-binding homology domain / Mg2+ transporter | ||||||
Function / homology | Function and homology information magnesium ion homeostasis / magnesium ion transmembrane transporter activity / basolateral plasma membrane / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Chen, Y.S. / Gehring, K. | ||||||
Citation | Journal: J. Biol. Chem. / Year: 2018 Title: The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+efflux activity. Authors: Chen, Y.S. / Kozlov, G. / Fakih, R. / Funato, Y. / Miki, H. / Gehring, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6dj3.cif.gz | 182.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6dj3.ent.gz | 148.3 KB | Display | PDB format |
PDBx/mmJSON format | 6dj3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dj/6dj3 ftp://data.pdbj.org/pub/pdb/validation_reports/dj/6dj3 | HTTPS FTP |
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-Related structure data
Related structure data | 6dfdSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 22727.084 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CNNM2, ACDP2 / Plasmid: pGEX-6P-1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q9H8M5 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.29 Å3/Da / Density % sol: 62.56 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 / Details: 0.1 M Bis-Tris pH 6.0, 0.5 M Na Citrate pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CLSI / Beamline: 08ID-1 / Wavelength: 1.0332 Å |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: Jan 22, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. obs: 14399 / % possible obs: 96.5 % / Redundancy: 8.7 % / Rsym value: 0.093 / Net I/σ(I): 19.8 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 5.4 % / Mean I/σ(I) obs: 0.8 / Num. unique obs: 318 / CC1/2: 0.609 / % possible all: 70.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6DFD Resolution: 2.6→47.9 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 2.6→47.9 Å
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