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- PDB-5jd3: Crystal structure of LAE5, an alpha/beta hydrolase enzyme from th... -

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Basic information

Entry
Database: PDB / ID: 5jd3
TitleCrystal structure of LAE5, an alpha/beta hydrolase enzyme from the metagenome of Lake Arreo, Spain
ComponentsLAE5
KeywordsHYDROLASE / alpha/beta hydrolase / esterase / metagenome
Function / homologySGNH hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / DI(HYDROXYETHYL)ETHER
Function and homology information
Biological speciesuncultured bacterium (environmental samples)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å
AuthorsStogios, P.J. / Xu, X. / Nocek, B. / Cui, H. / Yim, V. / Martinez-Martinez, M. / Alcaide, M. / Ferrer, M. / Savchenko, A.
CitationJournal: To Be Published
Title: To be published
Authors: Martinez-Martinez, M.
History
DepositionApr 15, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 4, 2016Provider: repository / Type: Initial release
Revision 1.1Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_prerelease_seq / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_oper_list.symmetry_operation

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: LAE5
B: LAE5
C: LAE5
D: LAE5
E: LAE5
F: LAE5
G: LAE5
H: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)214,78215
Polymers214,3318
Non-polymers4507
Water27,9411551
1
A: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,0294
Polymers26,7911
Non-polymers2383
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)26,8982
Polymers26,7911
Non-polymers1061
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)26,8272
Polymers26,7911
Non-polymers351
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: LAE5


Theoretical massNumber of molelcules
Total (without water)26,7911
Polymers26,7911
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)26,8272
Polymers26,7911
Non-polymers351
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: LAE5


Theoretical massNumber of molelcules
Total (without water)26,7911
Polymers26,7911
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: LAE5


Theoretical massNumber of molelcules
Total (without water)26,7911
Polymers26,7911
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)26,8272
Polymers26,7911
Non-polymers351
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
9
A: LAE5
D: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)53,8215
Polymers53,5832
Non-polymers2383
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2780 Å2
ΔGint-20 kcal/mol
Surface area17360 Å2
MethodPISA
10
B: LAE5
C: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)53,7244
Polymers53,5832
Non-polymers1422
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2890 Å2
ΔGint-27 kcal/mol
Surface area17520 Å2
MethodPISA
11
E: LAE5
H: LAE5
hetero molecules


Theoretical massNumber of molelcules
Total (without water)53,6544
Polymers53,5832
Non-polymers712
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2530 Å2
ΔGint-27 kcal/mol
Surface area17650 Å2
MethodPISA
12
F: LAE5

G: LAE5


Theoretical massNumber of molelcules
Total (without water)53,5832
Polymers53,5832
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation1_554x,y,z-11
Buried area2270 Å2
ΔGint-11 kcal/mol
Surface area17540 Å2
MethodPISA
Unit cell
Length a, b, c (Å)66.591, 129.452, 129.848
Angle α, β, γ (deg.)90.00, 97.49, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
LAE5


Mass: 26791.436 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) uncultured bacterium (environmental samples)
Plasmid: p15Tv lic / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
#2: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#3: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C4H10O3
#4: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1551 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.59 Å3/Da / Density % sol: 52.49 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 0.1 M Tris pH 8.5, 0.2 M ammonium sulfate, 25% (w/v) PEG3350, 1/70 chymotrypsin

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97918 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 11, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.3→29.725 Å / Num. obs: 93738 / % possible obs: 96.9 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.125 / Net I/σ(I): 9.86
Reflection shellResolution: 2.3→2.34 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.543 / % possible all: 93.4

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
HKL-3000data reduction
HKL-3000data scaling
PHENIXmodel building
Cootmodel building
MrBUMPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3RJT
Resolution: 2.3→29.725 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 24.89
RfactorNum. reflection% reflectionSelection details
Rfree0.2359 4375 5.03 %Random
Rwork0.1838 ---
obs0.1864 86908 89.43 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.3→29.725 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13716 0 23 1551 15290
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00414050
X-RAY DIFFRACTIONf_angle_d0.68419029
X-RAY DIFFRACTIONf_dihedral_angle_d13.6855139
X-RAY DIFFRACTIONf_chiral_restr0.032033
X-RAY DIFFRACTIONf_plane_restr0.0032525
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.32250.28061240.22412070X-RAY DIFFRACTION68
2.3225-2.34980.29081150.22832394X-RAY DIFFRACTION78
2.3498-2.37850.30021170.22052413X-RAY DIFFRACTION77
2.3785-2.40860.25341120.21972448X-RAY DIFFRACTION81
2.4086-2.44020.25951400.22672426X-RAY DIFFRACTION80
2.4402-2.47360.29961430.23222434X-RAY DIFFRACTION79
2.4736-2.5090.31481250.22822456X-RAY DIFFRACTION80
2.509-2.54640.27831240.21762487X-RAY DIFFRACTION80
2.5464-2.58620.30181210.22252444X-RAY DIFFRACTION81
2.5862-2.62850.25061270.22462502X-RAY DIFFRACTION81
2.6285-2.67380.28551350.20992518X-RAY DIFFRACTION82
2.6738-2.72240.27271320.23062552X-RAY DIFFRACTION84
2.7224-2.77470.26121530.22662610X-RAY DIFFRACTION84
2.7747-2.83130.25371420.222716X-RAY DIFFRACTION89
2.8313-2.89290.31581570.23062787X-RAY DIFFRACTION90
2.8929-2.96010.2921540.22482844X-RAY DIFFRACTION93
2.9601-3.0340.2491640.20552918X-RAY DIFFRACTION94
3.034-3.1160.26491570.19172910X-RAY DIFFRACTION96
3.116-3.20760.24491900.18652956X-RAY DIFFRACTION97
3.2076-3.3110.2241570.18193062X-RAY DIFFRACTION99
3.311-3.42920.25421570.18673003X-RAY DIFFRACTION98
3.4292-3.56620.24781670.18333023X-RAY DIFFRACTION98
3.5662-3.72830.19341540.15613073X-RAY DIFFRACTION99
3.7283-3.92440.20231420.15533069X-RAY DIFFRACTION99
3.9244-4.16970.20181730.15373043X-RAY DIFFRACTION99
4.1697-4.49060.2111420.14143035X-RAY DIFFRACTION99
4.4906-4.94070.19781840.14263037X-RAY DIFFRACTION99
4.9407-5.65130.19261450.16133094X-RAY DIFFRACTION99
5.6513-7.1040.20771480.17383088X-RAY DIFFRACTION99
7.104-29.72750.21351740.1773121X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
13.42262.1264-2.3593.4438-3.33923.7321-0.069-0.1968-0.1175-0.0816-0.13910.05960.32420.40960.17260.28780.03110.02140.19170.01760.2959-4.0073-34.5853-5.6899
22.487-2.2322.0084.7686-1.7393.71290.0514-0.04-0.11770.0356-0.04610.25240.1336-0.2082-0.00990.20620.01540.04650.188-0.0330.1828-4.9723-33.1604-5.239
34.35944.6266-5.17337.4741-6.88087.6036-0.03260.18-0.14140.41020.0633-0.1613-0.4829-0.24040.00630.17480.0506-0.01870.1932-0.01750.1871-5.6838-17.9437-10.9884
45.29852.7594-1.07283.9639-0.97671.6462-0.20940.11080.0713-0.11840.09410.1838-0.1814-0.14650.12110.34990.06880.03510.1743-0.02450.1863-8.1899-15.4656-5.1547
58.78823.3795-5.72295.8956-4.72845.11660.24610.2579-0.42861.44220.0128-0.0015-0.8002-0.4292-0.40420.458-0.03240.06570.1929-0.06140.3112-11.7066-13.58741.0589
61.09880.5905-0.38830.8009-0.84011.0425-0.0437-0.16170.0387-0.0076-0.0392-0.03120.12640.04990.08780.33680.0005-0.00010.2168-0.01830.20485.4037-21.29141.3089
72.2358-1.97491.81816.8223-5.26557.72370.31450.1408-0.2898-0.5735-0.29520.39650.37990.5452-0.04520.21730.0217-0.00960.3183-0.05840.29941.7176-4.1228.5011
89.92923.47183.16783.9348-2.90447.2435-0.0149-1.08710.10261.12360.11570.2107-0.2263-0.0638-0.04970.4110.08150.06280.3137-0.00490.3468-3.3477.985733.7058
94.00421.8524-1.86943.8858-0.98673.93220.0243-0.1362-0.10420.32610.1145-0.1916-0.00780.1685-0.13040.20120.0452-0.00420.1792-0.04670.16692.708-0.793429.6845
104.9712-4.33442.97037.7118-7.28427.366-0.03970.05720.2929-0.0357-0.1509-0.4284-0.06590.46220.19060.2656-0.03390.02390.2282-0.08810.16079.3225.277812.4338
111.9189-0.13460.65510.0134-0.10923.16570.11050.2771-0.1857-0.0359-0.0387-0.04810.14270.2426-0.07770.34310.02310.03020.2322-0.04090.2520.4675-5.071511.2408
122.0213-1.7982-0.22741.8750.04162.88560.1094-0.0209-0.260.11190.01120.3326-0.21060.0975-0.14330.2453-0.02910.04410.197-0.03420.239-10.5318-2.700917.9566
134.84792.53482.72624.41393.65837.0560.1679-0.0233-0.10760.30240.057-0.18070.2818-0.2377-0.17840.18320.02870.04160.20630.04390.1407-28.1892-0.918127.2739
144.6076-1.5041-2.95932.73672.00485.8854-0.02880.27480.0055-0.07210.06650.09870.1339-0.2776-0.05510.2223-0.0217-0.0020.17070.04310.2134-26.5341.037726.0491
150.49530.83861.55341.77042.35556.4525-0.07830.1510.00610.0884-0.0110.2165-0.1819-0.19070.10960.2490.00960.05950.3430.01780.2829-33.58523.074538.5697
164.24821.07754.03482.33071.69855.177-0.0674-0.3512-0.07570.1075-0.07260.3568-0.062-0.63840.15210.2714-0.03690.0640.27020.05110.2107-36.9532-2.54740.5972
178.05071.98585.76471.31852.34085.75680.2467-0.0739-0.51060.1613-0.00660.08440.5693-0.2906-0.24830.4168-0.06920.06320.39110.06140.2997-31.7319-8.324547.5119
182.52492.25132.83582.86622.38645.69720.1134-0.1044-0.11880.1322-0.00460.03840.2998-0.053-0.11720.19360.02620.04230.18620.02240.1937-17.6765-6.627239.098
195.3062-2.1274-5.11872.74833.19245.67950.47050.49761.07081.0563-0.1017-0.7176-0.9947-1.9824-0.40760.4251-0.0195-0.04720.46250.0790.34725.0589-28.457310.7256
206.3769-0.86281.690.42721.06325.8067-0.1990.37990.4817-0.2397-0.1655-0.193-0.7456-0.02770.37170.3466-0.00140.06210.23860.05180.363423.5778-34.4461-7.0895
215.5416-0.9105-1.90246.8290.15071.2327-0.0211-0.18570.21670.49410.024-0.2529-0.12960.0529-0.02230.24470.026-0.05250.17450.03090.161318.2289-28.79621.9837
224.849-0.5461.72876.69494.07978.362-0.06960.23640.0974-0.50930.1737-0.6443-0.52130.7704-0.13210.2528-0.01950.10120.31270.08090.349933.5477-39.9918-4.2239
233.30494.24271.97157.78024.56323.9472-0.05670.1919-0.2015-0.01390.1745-0.56560.01190.4488-0.16020.26910.0335-0.02090.27240.06740.278530.8948-50.06-0.2312
240.580.4273-0.251.6014-0.21710.78370.0498-0.203-0.03460.1718-0.0769-0.12030.015-0.0040.020.21490.0002-0.0070.23760.00980.2116.7208-48.47025.3953
254.47142.28932.90044.89231.62677.93070.1339-0.1143-0.02130.4465-0.1228-0.2046-0.1483-0.2452-0.01120.22830.04140.04490.17060.01850.2211-3.324324.226262.6897
266.6243-5.28025.33615.2141-3.12385.6358-1.5667-0.08751.17990.35610.03770.3253-1.4728-0.39251.19260.77140.09370.01020.4155-0.0840.5891-3.573632.759952.7846
272.7426-1.4901-1.26192.22830.6812.81150.15490.04870.18040.0949-0.0505-0.2982-0.29930.0477-0.13350.2681-0.03330.01290.21350.03350.2843-1.675225.004261.347
285.90694.13595.17815.94034.39244.7514-0.12120.3589-0.3497-0.41440.2716-0.6041-0.10650.4956-0.210.30830.01340.10230.23040.03860.22733.15695.540555.2337
291.77380.07310.05091.4410.9030.68970.1043-0.2282-0.14360.2146-0.0995-0.10110.1447-0.116-0.01130.3781-0.01180.03260.26170.04480.2337-7.71187.874164.4107
302.67930.93661.79444.08510.07165.375-0.2891-0.16990.02310.13670.03610.1551-0.5726-0.24910.22780.21090.01760.03680.20410.03150.2665-13.84717.972564.8989
315.05613.4291-2.32982.5149-2.39436.26440.01710.14660.42880.20030.31070.2045-0.35080.2817-0.34040.3391-0.0490.07050.2996-0.0430.237928.6419-4.1212-30.7502
328.38413.2686-4.39678.147-3.67842.8955-0.59651.1187-0.0359-1.40730.7580.20310.9651-0.0624-0.09260.5263-0.09140.05090.3616-0.08010.421225.7606-16.9055-35.4339
331.3444-1.00871.9332.8311-1.19524.15610.02670.15180.0677-0.1785-0.0525-0.2687-0.17980.3610.0470.2476-0.04050.04750.2283-0.01650.235629.844-7.349-31.0755
342.63253.2926-2.55386.0781-5.66135.44320.26210.0448-0.08260.0452-0.0465-0.0313-0.07070.5084-0.03640.28220.02080.01920.2347-0.05620.219925.9031-13.7999-9.6012
354.03420.8268-1.17751.8973-1.19072.25990.0486-0.27540.26720.26980.0066-0.2125-0.14770.2824-0.06930.3186-0.0214-0.03710.2519-0.06310.252530.0352-1.7494-14.1279
364.02081.4883-2.45991.5875-0.28133.73010.13370.0240.1033-0.03720.02680.0576-0.22010.0934-0.18280.24260.0144-0.03040.20720.00480.212314.1692-1.6327-22.144
378.0015-2.787-2.38497.29581.99046.90630.38390.08230.62890.0796-0.1003-0.6375-0.9524-0.9212-0.18770.34360.04960.04850.25710.03020.334-20.81361.618594.9797
386.00020.42292.71496.2535-1.50713.8723-0.2219-0.69020.0640.54960.3747-0.59750.6688-0.3783-0.15170.46210.09470.02530.3417-0.01170.2647-15.4785-15.287593.5832
390.56390.24810.1711.36730.53592.09960.014-0.05770.01640.07640.00970.14360.0419-0.2792-0.01310.24170.01730.01910.23480.04050.222-17.6335-9.989987.8649
404.6773-5.1772-4.64745.67575.14724.6044-0.3565-0.1568-0.77830.0981-0.08160.87120.5186-0.53720.45850.4993-0.0614-0.02190.4323-0.00650.3937-26.0875-11.327874.3647
410.7149-0.04110.07021.2173-0.22181.0155-0.0020.14590.1269-0.0790.01870.017-0.0828-0.0259-0.01720.3042-0.0230.01820.23180.01710.2207-9.12-4.918677.0083
428.1109-0.8671-0.94675.6556-0.06325.46280.6636-0.04580.55010.565-0.4655-0.0444-1.9224-0.0686-0.01860.4513-0.0216-0.01310.29920.06030.2574-10.19684.983190.1863
434.96852.1753-1.31696.4759-3.15511.5706-0.0422-0.0583-0.2211-0.1269-0.21320.2390.38250.04530.23210.3413-0.00820.04380.2515-0.0450.2652-31.056124.980455.9182
441.7818-1.52820.04315.0964-0.39632.1608-0.1076-0.1327-0.08910.00390.07030.51580.3274-0.3643-0.00660.256-0.05040.07530.2523-0.02110.239-33.311327.825457.3576
454.57376.0141-6.46929.396-7.64539.68660.04160.71760.3268-0.17310.38860.46310.0009-0.9929-0.48080.2960.00830.01820.3291-0.00790.2723-33.013251.78850.0899
465.72892.5374-0.274.472-0.1921.4910.0807-0.16390.22350.2335-0.0890.7471-0.093-0.15980.01290.34420.07230.13490.271-0.02610.3216-35.297542.346962.0259
474.35254.4142-3.8956.3158-2.18755.0758-0.2263-0.6710.09182.27540.2946-0.1039-1.3755-0.3363-0.25530.57280.05030.11280.5088-0.03840.5031-38.971144.445166.9231
483.61522.0357-1.47672.5112-0.01242.31890.1415-0.18850.02980.3181-0.13330.1449-0.01010.0287-0.04070.40340.03380.05860.2781-0.00270.2017-22.033336.915265.6648
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 0:31)
2X-RAY DIFFRACTION2(chain A and resid 32:81)
3X-RAY DIFFRACTION3(chain A and resid 82:105)
4X-RAY DIFFRACTION4(chain A and resid 106:153)
5X-RAY DIFFRACTION5(chain A and resid 154:165)
6X-RAY DIFFRACTION6(chain A and resid 166:217)
7X-RAY DIFFRACTION7(chain B and resid 0:18)
8X-RAY DIFFRACTION8(chain B and resid 19:36)
9X-RAY DIFFRACTION9(chain B and resid 37:87)
10X-RAY DIFFRACTION10(chain B and resid 88:128)
11X-RAY DIFFRACTION11(chain B and resid 129:183)
12X-RAY DIFFRACTION12(chain B and resid 184:217)
13X-RAY DIFFRACTION13(chain C and resid 0:23)
14X-RAY DIFFRACTION14(chain C and resid 24:74)
15X-RAY DIFFRACTION15(chain C and resid 75:104)
16X-RAY DIFFRACTION16(chain C and resid 105:141)
17X-RAY DIFFRACTION17(chain C and resid 142:168)
18X-RAY DIFFRACTION18(chain C and resid 169:217)
19X-RAY DIFFRACTION19(chain D and resid 0:8)
20X-RAY DIFFRACTION20(chain D and resid 9:31)
21X-RAY DIFFRACTION21(chain D and resid 32:59)
22X-RAY DIFFRACTION22(chain D and resid 60:83)
23X-RAY DIFFRACTION23(chain D and resid 84:131)
24X-RAY DIFFRACTION24(chain D and resid 132:217)
25X-RAY DIFFRACTION25(chain E and resid 0:22)
26X-RAY DIFFRACTION26(chain E and resid 23:31)
27X-RAY DIFFRACTION27(chain E and resid 32:88)
28X-RAY DIFFRACTION28(chain E and resid 89:124)
29X-RAY DIFFRACTION29(chain E and resid 125:192)
30X-RAY DIFFRACTION30(chain E and resid 193:217)
31X-RAY DIFFRACTION31(chain F and resid 0:19)
32X-RAY DIFFRACTION32(chain F and resid 20:35)
33X-RAY DIFFRACTION33(chain F and resid 36:86)
34X-RAY DIFFRACTION34(chain F and resid 87:107)
35X-RAY DIFFRACTION35(chain F and resid 108:170)
36X-RAY DIFFRACTION36(chain F and resid 171:217)
37X-RAY DIFFRACTION37(chain G and resid 1:10)
38X-RAY DIFFRACTION38(chain G and resid 11:31)
39X-RAY DIFFRACTION39(chain G and resid 32:103)
40X-RAY DIFFRACTION40(chain G and resid 104:128)
41X-RAY DIFFRACTION41(chain G and resid 129:206)
42X-RAY DIFFRACTION42(chain G and resid 207:217)
43X-RAY DIFFRACTION43(chain H and resid 0:32)
44X-RAY DIFFRACTION44(chain H and resid 33:94)
45X-RAY DIFFRACTION45(chain H and resid 95:112)
46X-RAY DIFFRACTION46(chain H and resid 113:153)
47X-RAY DIFFRACTION47(chain H and resid 154:165)
48X-RAY DIFFRACTION48(chain H and resid 166:217)

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