温度: 277.15 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.57 詳細: Crystals of L89S-FeBMb were set up and grown at 4 C on hanging drops containing 0.1M MES pH 6.57, 0.2M NaOAC.3H2O, 30% PEG 6K as well buffer. Drops contained an equal volume of 1 mM L89S- ...詳細: Crystals of L89S-FeBMb were set up and grown at 4 C on hanging drops containing 0.1M MES pH 6.57, 0.2M NaOAC.3H2O, 30% PEG 6K as well buffer. Drops contained an equal volume of 1 mM L89S-FeBMb in 20 mM TRIS.H2SO4 pH 8 and the well buffer. Prior to mounting, the L89S-FeBMb crystals were soaked in buffer containing 0.1M MES pH 6.0, 0.2M NaOAC.3H2O, 30% PEG 6K for 30 min and then frozen in a cryoprotectant solution of 50 mM MES pH 6.0, and 30% PEG 400