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- PDB-6crn: Structure of the USP15 deubiquitinase domain in complex with a hi... -

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Basic information

Entry
Database: PDB / ID: 6crn
TitleStructure of the USP15 deubiquitinase domain in complex with a high-affinity first-generation Ubv
Components
  • Ubiquitin carboxyl-terminal hydrolase 15
  • Ubiquitin variant 15.2
KeywordsSIGNALING PROTEIN / deubiuqitination / Ubv / high-affinity / inhibition
Function / homology
Function and homology information


regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator / negative regulation of antifungal innate immune response / protein K27-linked deubiquitination / positive regulation of RIG-I signaling pathway / ubiquitin-modified histone reader activity / monoubiquitinated protein deubiquitination / transforming growth factor beta receptor binding / deubiquitinase activity / K48-linked deubiquitinase activity / protein deubiquitination ...regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator / negative regulation of antifungal innate immune response / protein K27-linked deubiquitination / positive regulation of RIG-I signaling pathway / ubiquitin-modified histone reader activity / monoubiquitinated protein deubiquitination / transforming growth factor beta receptor binding / deubiquitinase activity / K48-linked deubiquitinase activity / protein deubiquitination / transcription elongation-coupled chromatin remodeling / SMAD binding / BMP signaling pathway / transforming growth factor beta receptor signaling pathway / Downregulation of TGF-beta receptor signaling / negative regulation of transforming growth factor beta receptor signaling pathway / UCH proteinases / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / nuclear body / Ub-specific processing proteases / cysteine-type endopeptidase activity / mitochondrion / proteolysis / nucleoplasm / identical protein binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Ubiquitin-like domain, USP-type / Ubiquitin-like domain / Peptidase C19, ubiquitin-specific peptidase, DUSP domain / DUSP-like superfamily / DUSP domain / DUSP domain profile. / Domain in ubiquitin-specific proteases. / Ubiquitin carboxyl-terminal hydrolase, C-terminal / Ubiquitin-specific protease C-terminal / : ...Ubiquitin-like domain, USP-type / Ubiquitin-like domain / Peptidase C19, ubiquitin-specific peptidase, DUSP domain / DUSP-like superfamily / DUSP domain / DUSP domain profile. / Domain in ubiquitin-specific proteases. / Ubiquitin carboxyl-terminal hydrolase, C-terminal / Ubiquitin-specific protease C-terminal / : / RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta / Ubiquitin specific protease (USP) domain signature 2. / Ubiquitin specific protease (USP) domain signature 1. / Ubiquitin specific protease, conserved site / Peptidase C19, ubiquitin carboxyl-terminal hydrolase / Ubiquitin carboxyl-terminal hydrolase / Ubiquitin specific protease domain / Ubiquitin specific protease (USP) domain profile. / Cysteine proteinases / Cathepsin B; Chain A / Papain-like cysteine peptidase superfamily / Ubiquitin-like domain superfamily / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
Ubiquitin carboxyl-terminal hydrolase 15
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsSinger, A.U. / Teyra, J. / Boehmelt, G. / Lenter, M. / Sicheri, F. / Sidhu, S.S.
CitationJournal: Structure / Year: 2019
Title: Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15.
Authors: Teyra, J. / Singer, A.U. / Schmitges, F.W. / Jaynes, P. / Kit Leng Lui, S. / Polyak, M.J. / Fodil, N. / Krieger, J.R. / Tong, J. / Schwerdtfeger, C. / Brasher, B.B. / Ceccarelli, D.F.J. / ...Authors: Teyra, J. / Singer, A.U. / Schmitges, F.W. / Jaynes, P. / Kit Leng Lui, S. / Polyak, M.J. / Fodil, N. / Krieger, J.R. / Tong, J. / Schwerdtfeger, C. / Brasher, B.B. / Ceccarelli, D.F.J. / Moffat, J. / Sicheri, F. / Moran, M.F. / Gros, P. / Eichhorn, P.J.A. / Lenter, M. / Boehmelt, G. / Sidhu, S.S.
History
DepositionMar 19, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 23, 2019Provider: repository / Type: Initial release
Revision 1.1Feb 13, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Apr 17, 2019Group: Data collection / Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first
Revision 1.3Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ubiquitin carboxyl-terminal hydrolase 15
B: Ubiquitin carboxyl-terminal hydrolase 15
C: Ubiquitin carboxyl-terminal hydrolase 15
D: Ubiquitin carboxyl-terminal hydrolase 15
E: Ubiquitin variant 15.2
F: Ubiquitin variant 15.2
G: Ubiquitin variant 15.2
H: Ubiquitin variant 15.2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,39712
Polymers200,1358
Non-polymers2624
Water5,711317
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)41.770, 98.942, 122.043
Angle α, β, γ (deg.)66.49, 88.31, 78.10
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
Ubiquitin carboxyl-terminal hydrolase 15 / Deubiquitinating enzyme 15 / Ubiquitin thioesterase 15 / Ubiquitin-specific-processing protease 15 ...Deubiquitinating enzyme 15 / Ubiquitin thioesterase 15 / Ubiquitin-specific-processing protease 15 / Unph-2 / Unph4


Mass: 40959.340 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: USP15, KIAA0529 / Plasmid: pHH0103
Details (production host): N-terminal GST with a His tag and a TEV cleavage site
Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9Y4E8, ubiquitinyl hydrolase 1
#2: Protein
Ubiquitin variant 15.2 / Ubv 15.2


Mass: 9074.376 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: This is a Ubv (ubiquitin variant) selected by phage display to bind the USP15 USP domain with high affinity
Source: (gene. exp.) Homo sapiens (human) / Gene: Ubb / Plasmid: modified pET53
Details (production host): N-terminal His tag, TEV cleavage site
Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: Zn
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 317 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.25 Å3/Da / Density % sol: 45.32 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop
Details: 24% PDG3350, 200 mM Potassium Citrate tribasic, 100 mM MES 6.5, cryoprotected in 21% PEG3350, 100 mM Potassium Citrate tribasic, 100 mM MES 6.5, 25% glycerol

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 15, 2016 / Details: Mirrors
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 2.11→111.72 Å / Num. obs: 92086 / % possible obs: 91.2 % / Redundancy: 2.1 % / CC1/2: 0.993 / Rmerge(I) obs: 0.08 / Net I/σ(I): 7.6
Reflection shellResolution: 2.11→2.14 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.815 / Mean I/σ(I) obs: 0.8 / Num. unique obs: 612 / CC1/2: 0.419 / % possible all: 63.5

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Processing

Software
NameVersionClassification
PHENIX(1.13_2998: ???)refinement
MOSFLMdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB 2Y6E and a SwissModeller model of the 15.13 Ubvc

2y6e


Resolution: 2.5→111.72 Å / SU ML: 0.29 / Cross valid method: FREE R-VALUE / σ(F): 1.99 / Phase error: 25.72 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2355 2878 5.12 %
Rwork0.188 --
obs0.1904 56174 92.78 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.5→111.72 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13091 0 4 317 13412
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00813451
X-RAY DIFFRACTIONf_angle_d1.25318217
X-RAY DIFFRACTIONf_dihedral_angle_d8.94110113
X-RAY DIFFRACTIONf_chiral_restr0.0791978
X-RAY DIFFRACTIONf_plane_restr0.0072358
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.20860.0396-0.05170.2087-0.10090.3124-0.0198-0.1114-0.06610.1660.00620.0432-0.0166-0.0205-00.15050.01710.00230.20080.06180.114377.34-161.8295-10.8572
20.17230.13420.29290.17580.08970.0552-0.3661-0.0807-0.206-0.27270.2911-0.3421-0.1993-0.176-00.06650.0443-0.10860.17910.02780.077174.1554-151.6502-25.3594
30.18840.28280.00550.23410.12260.15690.0398-0.0125-0.0962-0.0487-0.0577-0.05470.0352-0.0432-00.1205-0.00880.02090.1757-0.01850.207792.2587-184.5464-51.255
40.03-0.1153-0.19550.3-0.08570.33480.0961-0.13740.1246-0.0729-0.12130.0286-0.1488-0.0459-00.20080.0258-0.01730.12840.02190.180495.3748-165.0343-55.5362
5-0.0283-0.046-0.02490.0811-0.02710.05180.0720.10460.113-0.1662-0.32330.0079-0.01320.006-00.1230.0003-0.06290.1744-0.10.292291.4101-106.9936-66.5225
60.041-0.0744-0.07950.1351-0.01110.07450.09890.04820.2815-0.0277-0.3159-0.07770.17660.082500.1703-0.0571-0.01130.1329-0.09460.238892.9553-108.7043-56.9097
7-0.1130.00680.0941-0.0595-0.12660.1027-0.03860.969-0.34190.2256-0.75990.39060.3852-0.4326-00.3379-0.27670.1423-0.36340.06170.195193.8341-128.8158-47.3313
80.02750.0469-0.00880.04610.0640.0271-0.08560.50130.4995-0.1176-0.5588-0.46430.71910.240800.1077-0.122-0.10810.1181-0.26880.032696.2921-125.6322-68.9821
90.006-0.01120.0027-0.00420.00310.00750.0251-0.04090.0694-0.00260.05710.0335-0.1392-0.126600.19330.0631-0.04380.3034-0.0050.320567.3843-117.404-94.7592
100.0165-0.0911-0.01320.0661-0.05230.06590.1957-0.00790.0486-0.2955-0.18760.0657-0.1526-0.283500.19380.0935-0.10830.04560.5642-0.923574.6715-126.7211-102.5272
110.00220.0013-0.0080.0050.01030.0046-0.01620.22110.0239-0.04680.0525-0.14450.11330.0164-00.380.09050.06330.21850.00480.164789.8362-148.9249-102.221
12-0.0180.0058-0.06870.00140.0079-0.0417-0.21330.13230.09820.0486-0.37620.16040.3157-0.013500.2737-0.25320.4689-0.24780.4759-0.427676.6781-148.1158-88.6287
130.00320.00890.00240.0089-0.00430.0038-0.0482-0.0155-0.00060.0749-0.10390.0344-0.05440.003700.498-0.06980.46330.5749-0.55930.446472.4757-161.9224-96.1636
140.0192-0.0151-0.00710.0921-0.07910.0173-0.0934-0.0196-0.08230.14160.09350.01370.1316-0.0098-00.16390.00140.01830.19260.01830.15771.833-141.7351-85.0688
150.0242-0.04150.01620.09020.04310.0946-0.12840.05690.03060.09060.1075-0.05540.0622-0.108300.07950.0195-0.010.1898-0.0090.147873.3847-128.7381-83.9617
160.0026-0.0042-0.0009-0.00180.00180.001-0.0423-0.05070.01590.0562-0.0607-0.02770.006-0.0194-00.6045-0.1025-0.09150.37450.05910.304196.2208-116.5001-28.5096
170.0004-0.00020.00040.0031-0.00120.00110.0030.00230.0036-0.00490.00750.0005-0.00340.010900.99350.066-0.05240.94460.24481.0817111.9438-118.6802-31.9919
18-0.0017-0.0033-0.0019-0.0022-0.00490.0042-0.0313-0.0734-0.05250.0006-0.1486-0.035-0.0199-0.063600.5535-0.0494-0.08110.455-0.00290.386496.4227-111.7546-28.1896
190.00260.0046-00.00390.0019-0.0008-0.036-0.0683-0.0477-0.028-0.0773-0.0149-0.0487-0.004-00.6422-0.1186-0.11160.38140.01610.4431103.0655-106.6213-28.6832
200.0010.0012-0.00060.0011-0.00020.0012-0.0081-0.0098-0.008-0.0176-0.0067-0.0062-0.0020.019900.617-0.1334-0.12890.42430.15420.7443110.3749-103.6375-32.5267
21-0.00170.00280.00020.0022-0.00090.003-0.03680.01270.00410.0372-0.0525-0.0338-0.03470.0202-00.5862-0.17630.04460.37520.09650.4498102.0076-111.8291-41.0332
220.0039-0.00160.00590.00190.00640.0118-0.00730.05510.01120.0935-0.08540.02790.0914-0.0600.546-0.0763-0.05770.28110.05730.375193.4377-110.7305-37.1148
230.0035-0.0003-0.0030.00050.0021-0.00060.02250.00320.0080.00180.0191-0.03210.00240.0513-00.4794-0.2291-0.05970.4551-0.04580.5262104.9914-114.941-36.574
24-0.0004-0.00280.00330.0068-0.0032-0.0013-0.0312-0.012-0.0235-0.0652-0.0974-0.08640.06220.026300.52010.05260.03860.2470.09930.3713102.8088-175.0527-82.4514
25-0.0023-0.00140.00330.00070.0055-0.00860.02140.09360.0509-0.0219-0.01690.00440.1441-0.1798-00.73570.09680.05480.3903-0.00360.48396.65-180.792-84.2462
26-0.0016-0.0027-0.00150.00240.00120.00160.0250.03350.00570.03110.0224-0.04880.05820.021700.83310.18120.28820.26290.1260.2593103.3962-185.9432-83.7652
27-0.0008-0.00370.00330.0023-0.00160.0026-0.1233-0.1197-0.023-0.0154-0.1197-0.1275-0.0248-0.065700.69770.29880.14890.38360.18970.5307105.1661-183.7488-74.4695
28-0.0021-0.0006-0.00820.00440.0080.0074-0.0137-0.1116-0.0285-0.0841-0.08280.0285-0.0923-0.059700.46220.08680.03550.22860.04490.18793.5139-181.3098-75.6602
290.00010.00050.00310.00130.00310.00130.04620.0111-0.03010.03080.0344-0.06040.0076-0.007800.68660.0819-0.08960.40930.11160.5245106.8445-179.7235-74.8834
300.00370.0002-0.001-0.00090.00160.00180.0232-0.0202-0.0207-0.04020.04970.01160.0356-0.028-00.7712-0.1254-0.0110.7104-0.15710.841274.3599-159.7803-111.3448
310.0019-0.00760.00210.0387-0.01460.0074-0.004-0.01240.0046-0.00160.00610.00610.00810.011101.0503-0.0461-0.13581.1979-0.03481.125660.0158-160.1687-106.1245
32-0.00130.0032-0.00270.00460.00630.00260.04910.035-0.03150.0243-0.01430.00380.0117-0.0006-01.0621-0.096-0.03570.689-0.0610.754974.8349-157.9481-115.8956
33-0.00030.00060.0050.0001-0.0010.0022-0.07320.0587-0.00540.0018-0.04120.0291-0.0052-0.009200.9414-0.0817-0.28970.7928-0.25690.714667.6268-155.8303-120.3505
340.00070.00030.00170.01340.0008-0.00020.02110.0066-0.0024-0.01010.01450.01260.0016-0.0168-01.4555-0.0804-0.26641.4095-0.00851.477960.6047-152.2594-119.6148
35-0.0001-0.00260.0020.00150.0009-0.00110.01290.0050.00990.02280.0090.02870.0013-0.0044-01.00310.3212-0.17170.814-0.19650.941869.3867-147.0351-107.8215
360.0014-0.00180.0120.00530.0077-0.0014-0.01270.02570.02210.00670.00380.07710.0394-0.0301-00.74870.09870.01360.4578-0.08920.520574.5716-150.6972-111.1926
370.001-0.0014-0.001-0.00130.00340.0015-0.00340.01490.00280.00010.01220.0036-0.0121-0.0366-00.8480.10570.11220.8734-0.23880.707974.8136-132.7942-0.7452
380.0043-0.00330.0009-0.00040.0068-0.00030.04890.0070.01270.01860.07080.0141-0.014-0.02200.9490.18580.15810.8172-0.18880.889768.5452-132.1681-0.1223
390.00960.0042-0.00210.0056-0.00180.0014-0.01320.01470.01550.0044-0.0081-0.0077-0.0127-0.004800.98290.16330.09420.8942-0.2650.685479.3625-139.60667.1959
40-0.0006-0.0003-0.0028-0.0014-0.00190.00120.0445-0.0240.0425-0.00160.05460.0444-0.0043-0.0065-00.96590.39180.25890.9961-0.27780.836365.9158-137.988.037
41-0.00040.0011-0.0005-0.00020.00060.00060.009-0.02060.0129-0.0357-0.0076-0.00440.0002-0.004500.793-0.00180.10570.9627-0.20340.903769.769-145.251-4.8093
420.0045-0.0037-0.00630.00150.00740.00870.0516-0.10460.0531-0.0434-0.02120.0099-0.0553-0.0613-00.6279-0.00460.10640.4954-0.06240.633477.444-142.59850.0389
430.0004-0.00060.00010.00150.0004-0.0022-0.00340.00650.009-0.0032-0.0084-0.0047-0.01-0.0182-00.6396-0.02850.10650.7235-0.10530.942264.4262-139.9266-3.1556
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 280 through 429 )
2X-RAY DIFFRACTION2chain 'A' and (resid 430 through 626 )
3X-RAY DIFFRACTION3chain 'B' and (resid 277 through 414 )
4X-RAY DIFFRACTION4chain 'B' and (resid 415 through 623 )
5X-RAY DIFFRACTION5chain 'C' and (resid 277 through 336 )
6X-RAY DIFFRACTION6chain 'C' and (resid 337 through 414 )
7X-RAY DIFFRACTION7chain 'C' and (resid 415 through 526 )
8X-RAY DIFFRACTION8chain 'C' and (resid 527 through 623 )
9X-RAY DIFFRACTION9chain 'D' and (resid 280 through 299 )
10X-RAY DIFFRACTION10chain 'D' and (resid 300 through 396 )
11X-RAY DIFFRACTION11chain 'D' and (resid 397 through 429 )
12X-RAY DIFFRACTION12chain 'D' and (resid 430 through 485 )
13X-RAY DIFFRACTION13chain 'D' and (resid 486 through 508 )
14X-RAY DIFFRACTION14chain 'D' and (resid 509 through 554 )
15X-RAY DIFFRACTION15chain 'D' and (resid 555 through 626 )
16X-RAY DIFFRACTION16chain 'E' and (resid -2 through 6 )
17X-RAY DIFFRACTION17chain 'E' and (resid 7 through 11 )
18X-RAY DIFFRACTION18chain 'E' and (resid 12 through 22 )
19X-RAY DIFFRACTION19chain 'E' and (resid 23 through 34 )
20X-RAY DIFFRACTION20chain 'E' and (resid 35 through 40 )
21X-RAY DIFFRACTION21chain 'E' and (resid 41 through 49 )
22X-RAY DIFFRACTION22chain 'E' and (resid 50 through 64 )
23X-RAY DIFFRACTION23chain 'E' and (resid 65 through 71 )
24X-RAY DIFFRACTION24chain 'F' and (resid -1 through 11 )
25X-RAY DIFFRACTION25chain 'F' and (resid 12 through 22 )
26X-RAY DIFFRACTION26chain 'F' and (resid 23 through 34 )
27X-RAY DIFFRACTION27chain 'F' and (resid 35 through 50 )
28X-RAY DIFFRACTION28chain 'F' and (resid 51 through 65 )
29X-RAY DIFFRACTION29chain 'F' and (resid 66 through 72 )
30X-RAY DIFFRACTION30chain 'G' and (resid -1 through 6 )
31X-RAY DIFFRACTION31chain 'G' and (resid 7 through 11 )
32X-RAY DIFFRACTION32chain 'G' and (resid 12 through 22 )
33X-RAY DIFFRACTION33chain 'G' and (resid 23 through 34 )
34X-RAY DIFFRACTION34chain 'G' and (resid 35 through 41 )
35X-RAY DIFFRACTION35chain 'G' and (resid 42 through 50 )
36X-RAY DIFFRACTION36chain 'G' and (resid 51 through 70 )
37X-RAY DIFFRACTION37chain 'H' and (resid -1 through 6 )
38X-RAY DIFFRACTION38chain 'H' and (resid 7 through 17 )
39X-RAY DIFFRACTION39chain 'H' and (resid 18 through 22 )
40X-RAY DIFFRACTION40chain 'H' and (resid 23 through 39 )
41X-RAY DIFFRACTION41chain 'H' and (resid 42 through 50 )
42X-RAY DIFFRACTION42chain 'H' and (resid 51 through 65 )
43X-RAY DIFFRACTION43chain 'H' and (resid 66 through 72 )

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