+Open data
-Basic information
Entry | Database: PDB / ID: 6cr1 | ||||||
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Title | adalimumab EFab | ||||||
Components |
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Keywords | IMMUNE SYSTEM / EFab / IgE | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / NITRATE ION / DI(HYDROXYETHYL)ETHER Function and homology information | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.521 Å | ||||||
Authors | Arndt, J.W. | ||||||
Citation | Journal: MAbs / Year: 2018 Title: EFab domain substitution as a solution to the light-chain pairing problem of bispecific antibodies. Authors: Cooke, H.A. / Arndt, J. / Quan, C. / Shapiro, R.I. / Wen, D. / Foley, S. / Vecchi, M.M. / Preyer, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6cr1.cif.gz | 174.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6cr1.ent.gz | 136.8 KB | Display | PDB format |
PDBx/mmJSON format | 6cr1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cr/6cr1 ftp://data.pdbj.org/pub/pdb/validation_reports/cr/6cr1 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Antibody | Mass: 22934.590 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster) | ||
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#2: Antibody | Mass: 25354.104 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster) | ||
#3: Chemical | ChemComp-PEG / | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60.34 % |
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Crystal grow | Temperature: 273 K / Method: vapor diffusion, sitting drop / pH: 7.2 / Details: 15% PEG 3350 and 0.25M potassium nitrate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.9793 Å |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Feb 15, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.52→30 Å / Num. obs: 96198 / % possible obs: 99.6 % / Redundancy: 7.4 % / Rmerge(I) obs: 0.115 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 1.52→1.54 Å / CC1/2: 0.684 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4NYL, 2WQR Resolution: 1.521→24.898 Å / SU ML: 0.16 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 16.17
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.521→24.898 Å
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Refine LS restraints |
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LS refinement shell |
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