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- PDB-6c4c: Crystal structure of 3-nitropropionate modified isocitrate lyase ... -

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Basic information

Entry
Database: PDB / ID: 6c4c
TitleCrystal structure of 3-nitropropionate modified isocitrate lyase from Mycobacterium tuberculosis with glyoxylate and pyruvate
ComponentsIsocitrate lyase 1
KeywordsLYASE
Function / homology
Function and homology information


methylisocitrate lyase / isocitrate lyase / methylisocitrate lyase activity / isocitrate lyase activity / glyoxylate cycle / tricarboxylic acid cycle / metal ion binding
Similarity search - Function
Isocitrate lyase / Isocitrate lyase family / Isocitrate lyase/phosphorylmutase, conserved site / Isocitrate lyase signature. / Phosphoenolpyruvate-binding domains / Pyruvate kinase-like domain superfamily / Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily / TIM Barrel / Alpha-Beta Barrel / Alpha Beta
Similarity search - Domain/homology
3-NITROPROPANOIC ACID / GLYOXYLIC ACID / PYRUVIC ACID / Isocitrate lyase 1
Similarity search - Component
Biological speciesMycobacterium tuberculosis (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å
AuthorsKreitler, D.F. / Ray, S. / Murkin, A.S. / Gulick, A.M.
Funding support United States, 2items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)CHE1255136 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM116957 United States
CitationJournal: ACS Chem. Biol. / Year: 2018
Title: The Nitro Group as a Masked Electrophile in Covalent Enzyme Inhibition.
Authors: Ray, S. / Kreitler, D.F. / Gulick, A.M. / Murkin, A.S.
History
DepositionJan 11, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 6, 2018Provider: repository / Type: Initial release
Revision 1.1Jun 27, 2018Group: Data collection / Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 2.0Mar 16, 2022Group: Atomic model / Author supporting evidence ...Atomic model / Author supporting evidence / Database references / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / database_2 / pdbx_audit_support / pdbx_struct_conn_angle / struct_conn / struct_conn_type
Item: _atom_site.auth_atom_id / _atom_site.label_atom_id ..._atom_site.auth_atom_id / _atom_site.label_atom_id / _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_audit_support.funding_organization / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr1_symmetry / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.ptnr3_symmetry / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr1_symmetry / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry / _struct_conn_type.id
Revision 2.1Oct 4, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 3.0Nov 15, 2023Group: Atomic model / Data collection / Derived calculations
Category: atom_site / chem_comp_atom ...atom_site / chem_comp_atom / chem_comp_bond / pdbx_struct_conn_angle / struct_conn
Item: _atom_site.auth_atom_id / _atom_site.label_atom_id ..._atom_site.auth_atom_id / _atom_site.label_atom_id / _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_1 / _chem_comp_bond.atom_id_2 / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _struct_conn.ptnr1_label_atom_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Isocitrate lyase 1
B: Isocitrate lyase 1
C: Isocitrate lyase 1
D: Isocitrate lyase 1
E: Isocitrate lyase 1
F: Isocitrate lyase 1
G: Isocitrate lyase 1
H: Isocitrate lyase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)392,72633
Polymers391,5558
Non-polymers1,17025
Water28,7701597
1
A: Isocitrate lyase 1
B: Isocitrate lyase 1
C: Isocitrate lyase 1
D: Isocitrate lyase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)196,29616
Polymers195,7784
Non-polymers51912
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area33740 Å2
ΔGint-222 kcal/mol
Surface area49230 Å2
MethodPISA
2
E: Isocitrate lyase 1
F: Isocitrate lyase 1
G: Isocitrate lyase 1
H: Isocitrate lyase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)196,42917
Polymers195,7784
Non-polymers65213
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area34230 Å2
ΔGint-198 kcal/mol
Surface area49210 Å2
MethodPISA
Unit cell
Length a, b, c (Å)143.760, 87.130, 152.990
Angle α, β, γ (deg.)90.000, 116.550, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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Protein , 1 types, 8 molecules ABCDEFGH

#1: Protein
Isocitrate lyase 1 / ICL1 / Isocitrase / Isocitratase / Methylisocitrate lyase / MICA


Mass: 48944.398 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman) (bacteria)
Strain: ATCC 35801 / TMC 107 / Erdman / Gene: icl1, ERDMAN_0512, Q643_00485 / Production host: Escherichia coli (E. coli)
References: UniProt: H8EVV4, isocitrate lyase, methylisocitrate lyase

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Non-polymers , 5 types, 1622 molecules

#2: Chemical ChemComp-GLV / GLYOXYLIC ACID / GLYOXALATE / GLYOXYLATE


Mass: 74.035 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C2H2O3
#3: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Mg
#4: Chemical
ChemComp-PYR / PYRUVIC ACID


Mass: 88.062 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H4O3
#5: Chemical ChemComp-3NP / 3-NITROPROPANOIC ACID


Mass: 119.076 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H5NO4
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1597 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.18 Å3/Da / Density % sol: 43.57 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8
Details: 50 mM EPPS, 100 mM MgCl2, 17.5%(w/v) PEG4000 (well solution); 10 mg/mL MtICL in 50 mM Tris pH 8.0, 100 mM NaCl, 5 mM MgSO4, 3 mM 3-nitropropionate, and 1 mM DTT (protein solution); 2 uL ...Details: 50 mM EPPS, 100 mM MgCl2, 17.5%(w/v) PEG4000 (well solution); 10 mg/mL MtICL in 50 mM Tris pH 8.0, 100 mM NaCl, 5 mM MgSO4, 3 mM 3-nitropropionate, and 1 mM DTT (protein solution); 2 uL total drop volume, 1:1 well solution:protein solution

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.97946 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 29, 2017
RadiationMonochromator: 0.97946 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97946 Å / Relative weight: 1
ReflectionResolution: 2.2→47.393 Å / Num. obs: 169258 / % possible obs: 98 % / Redundancy: 3.7 % / Biso Wilson estimate: 33.86 Å2 / CC1/2: 0.987 / Rmerge(I) obs: 0.198 / Rrim(I) all: 0.2316 / Net I/σ(I): 5.86
Reflection shellResolution: 2.2→2.279 Å / Redundancy: 3.8 % / Rmerge(I) obs: 1.63 / Mean I/σ(I) obs: 0.77 / Num. unique obs: 16914 / CC1/2: 0.377 / Rrim(I) all: 1.903 / % possible all: 99

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Processing

Software
NameVersionClassification
PHENIX1.10.1_2155refinement
XDSdata reduction
XSCALEdata scaling
PDB_EXTRACT3.24data extraction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6C4A

6c4a


Resolution: 2.2→47.393 Å / SU ML: 0.36 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 28.11 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2475 1999 1.18 %
Rwork0.192 167118 -
obs0.1926 169117 98.44 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 160.68 Å2 / Biso mean: 47.5217 Å2 / Biso min: 17.7 Å2
Refinement stepCycle: final / Resolution: 2.2→47.393 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms26436 0 103 1597 28136
Biso mean--39.61 35.68 -
Num. residues----3421
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00327118
X-RAY DIFFRACTIONf_angle_d0.49636892
X-RAY DIFFRACTIONf_chiral_restr0.0384051
X-RAY DIFFRACTIONf_plane_restr0.0034893
X-RAY DIFFRACTIONf_dihedral_angle_d12.25816010
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.2-2.2550.40121380.3424119161205499
2.255-2.3160.33371480.3146119501209899
2.316-2.38410.33581410.2924119421208399
2.3841-2.46110.30791420.2792118921203499
2.4611-2.5490.32881460.2618120281217499
2.549-2.65110.30061410.2406118461198798
2.6511-2.77170.31181390.2359117281186797
2.7717-2.91780.30641410.2233117601190197
2.9178-3.10060.29971450.21391206512210100
3.1006-3.340.2121460.1907120221216899
3.34-3.6760.25381390.1675119581209799
3.676-4.20760.18591400.1413117441188496
4.2076-5.30.16871460.1316121031224999
5.3-47.40370.20661470.1468121641231198
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.73180.0168-0.07970.8079-0.05351.13150.03270.0121-0.13260.04280.0005-0.03380.14990.0346-0.03590.23630.0062-0.03160.22210.01140.270174.6535-31.5195196.1562
21.1862-0.0196-0.17541.0499-0.19291.96530.01730.1920.0197-0.21740.0630.0945-0.0054-0.0273-0.08450.30360.0135-0.0150.2498-0.01520.276775.7117-29.1304175.1861
30.46090.1046-0.21361.14390.1911.13530.0503-0.05590.0680.0844-0.05050.29990.0324-0.20770.00260.2536-0.01170.010.29420.01880.360653.5047-20.5764201.1131
41.4868-1.4037-1.92046.65832.43488.98780.0763-0.33761.32460.19980.4842-0.4937-0.22550.4691-0.57530.6845-0.06250.19040.3099-0.04660.63478.329221.7802208.5914
50.59950.2506-0.0820.95470.1860.94440.0897-0.11080.26880.2739-0.03270.2049-0.2871-0.1291-0.05580.42570.03370.08830.2784-0.03220.407461.62251.4514212.6612
60.8325-0.6149-0.32392.42271.14451.40480.01760.02480.08630.1802-0.09750.4398-0.0292-0.2490.07680.28790.05060.03890.33630.04970.435348.0924-12.1208197.3638
77.11781.5721-1.81742.9002-0.47292.5473-0.15980.6879-0.4902-0.40110.1455-0.0175-0.1527-0.12080.01650.43980.0156-0.01750.37240.01830.303869.03-8.1548176.0866
89.03723.8419-5.0212.3258-2.85326.04751.02710.18011.8030.2013-0.05910.675-0.90390.0254-0.92880.79010.02140.11330.3490.08020.681377.968516.5052183.5197
90.9687-0.18850.67720.8932-0.20590.9764-0.0307-0.02760.26420.05980.0249-0.0639-0.28330.17780.02030.3466-0.08020.050.3385-0.00230.293192.49521.4224193.882
100.4598-0.5680.01391.27321.49775.5723-0.0773-0.0690.1795-0.23730.1266-0.0373-0.60420.3911-0.06360.3309-0.06990.02160.28060.00790.331589.50171.3227200.53
110.6623-0.4841-0.11951.610.5421.54510.05030.08810.1463-0.14590.022-0.1889-0.24450.364-0.06810.3452-0.07770.03880.36910.02220.294499.8292-7.0001178.7376
120.62050.4763-0.47811.0164-1.20373.20.0717-0.1590.05570.1006-0.0799-0.2501-0.3120.49130.00860.327-0.09040.00150.487-0.02770.4173109.7523-5.813203.8595
136.4432-2.187-1.59681.63780.84281.4913-0.2808-0.89930.05550.50150.17670.0626-0.01220.17180.10020.6703-0.15430.02240.6119-0.08680.411588.61033.4323225.5549
140.83650.2657-0.29210.62940.10581.29040.0278-0.2328-0.17710.1555-0.0378-0.03560.0980.25730.01430.29460.0043-0.02840.34520.05370.279285.6393-26.429217.1266
151.51360.3430.05750.77030.41991.19480.1081-0.42560.15420.3336-0.0809-0.0592-0.19340.1626-0.02790.5425-0.08690.00360.55220.01940.316585.6403-16.3161235.1151
160.46940.3315-0.06850.82910.0770.50760.0088-0.069-0.05360.0451-0.0121-0.2138-0.10720.4005-0.00430.2938-0.0148-0.04830.49340.00230.347105.8854-16.303203.0892
173.07490.85030.9331.855-1.10012.51480.1735-0.3336-0.07730.16450.0424-0.36240.02510.5284-0.250.3710.0688-0.05120.4832-0.06520.386866.971812.2825157.7334
180.7611-0.3238-0.30630.80120.21211.63020.02170.05840.07510.08010.0554-0.0648-0.29490.1221-0.07140.3359-0.01250.03630.2078-0.00170.265147.793223.0477146.9673
190.98780.0147-0.83240.9103-0.04840.8912-0.0071-0.22320.07570.3010.06250.1358-0.09630.1824-0.06170.53520.00910.03170.3181-0.02390.318744.036819.9088169.9429
205.6686-2.2327-0.55143.31981.15711.30450.152-0.54410.53160.33390.1699-0.093-0.44380.1577-0.3110.6695-0.06240.11570.3282-0.01650.318947.543635.2757168.0944
211.33190.34270.38042.37810.01731.9380.15-0.02230.2666-0.00250.09560.0514-0.6930.1583-0.22970.6556-0.03390.12060.2837-0.03590.362244.107938.7142159.7668
220.6593-0.2713-0.15592.05-1.26254.48570.11770.20760.3452-0.04140.09320.1065-0.7409-0.5054-0.23750.58710.12930.09280.35470.09380.436429.377136.4995134.051
231.5639-0.2268-0.67830.27530.82082.2818-0.03230.26630.1787-0.14040.1147-0.0686-0.13480.0906-0.09360.50080.00030.06490.34060.02270.311947.280125.2132120.0931
242.203-0.4310.95660.72440.90152.6121-0.05280.4756-0.3094-0.25770.13450.42710.5742-0.7529-0.13240.5165-0.263-0.06170.7098-0.01580.570112.6086-2.8009126.3764
250.9276-0.04-0.46570.63560.39172.57820.0660.31530.0887-0.05630.01160.241-0.1951-0.4582-0.07240.29490.0251-0.00530.32440.05610.30726.484916.4909131.5325
261.80910.641-0.74331.2815-0.19041.5327-0.04080.56460.052-0.37910.11310.15680.0218-0.4399-0.07710.5001-0.0346-0.06290.55830.03590.310727.660314.1346110.466
271.09780.33320.45010.8070.43152.5510.03050.27590.2616-0.02090.05810.2345-0.5004-0.3207-0.07530.51990.14450.07910.40970.14070.397527.907233.7032130.0406
281.55811.1458-1.25650.7996-0.96635.18660.13620.06370.20220.351-0.04920.2811-0.413-0.2581-0.08960.41950.11110.09990.27530.01460.349623.706224.3446160.2725
294.00890.0405-1.15973.0770.5844.1083-0.02210.0665-0.01860.23380.05410.59080.0912-0.8005-0.07250.3311-0.00080.05960.47040.02160.47839.80564.3948155.2404
301.19930.1343-0.21540.99340.06141.5275-0.15780.1205-0.21390.05670.0650.23380.4247-0.21130.09380.4314-0.03920.08280.2454-0.01250.325229.9331-7.8109148.4773
310.80790.24960.11630.8560.07911.5055-0.0964-0.0804-0.11060.33330.06380.02530.1320.03270.03370.58390.0420.12530.2850.01270.391731.3074-1.1653170.9669
321.3017-0.7101-0.11852.23570.40770.908-0.1187-0.1748-0.29930.35660.01980.23740.4321-0.04580.10940.7668-0.01840.16010.30440.01970.444330.4296-19.536166.4281
331.05320.4584-0.591.35481.03592.9926-0.2573-0.0031-0.5291-0.08640.09290.01090.72080.15640.15630.78210.07570.16120.2790.00740.486449.3281-22.8789140.0659
341.2658-0.54160.38082.7383-2.01052.67210.0160.3073-0.2266-0.5299-0.0780.10190.895-0.18080.06970.705-0.11930.05520.437-0.12320.396733.8965-14.2271122.6378
353.9027-0.4384-0.82461.5739-1.62783.13990.11190.16030.2421-0.0977-0.1534-0.2384-0.02120.3902-0.01480.3844-0.0290.07330.35510.01070.320867.473514.8424128.131
360.31080.48290.10790.6725-0.12911.8112-0.09340.0111-0.1351-0.10690.0088-0.12980.47240.25480.07960.42420.08450.07340.2673-0.02320.320754.0563-3.4897137.6562
370.59260.21420.21631.05010.22461.9236-0.13120.2356-0.2191-0.26320.0181-0.02410.48440.07110.1160.59890.01040.10530.381-0.05240.349453.7514-5.4685115.881
381.2551-0.0007-0.37370.8086-0.39640.6525-0.14710.1607-0.3192-0.1687-0.0343-0.08830.50710.04620.1790.81990.05360.14270.3019-0.07810.479549.418-22.7981139.3775
391.66440.1054-0.41192.05470.67392.3078-0.0947-0.2013-0.27530.481-0.0354-0.00510.72360.37250.08260.54670.10880.00140.3530.04990.328452.6156-6.5507164.2776
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 0 through 174 )A0 - 174
2X-RAY DIFFRACTION2chain 'A' and (resid 175 through 325 )A175 - 325
3X-RAY DIFFRACTION3chain 'A' and (resid 326 through 427 )A326 - 427
4X-RAY DIFFRACTION4chain 'B' and (resid 1 through 19 )B1 - 19
5X-RAY DIFFRACTION5chain 'B' and (resid 20 through 319 )B20 - 319
6X-RAY DIFFRACTION6chain 'B' and (resid 320 through 386 )B320 - 386
7X-RAY DIFFRACTION7chain 'B' and (resid 387 through 427 )B387 - 427
8X-RAY DIFFRACTION8chain 'C' and (resid 1 through 19 )C1 - 19
9X-RAY DIFFRACTION9chain 'C' and (resid 20 through 115 )C20 - 115
10X-RAY DIFFRACTION10chain 'C' and (resid 116 through 173 )C116 - 173
11X-RAY DIFFRACTION11chain 'C' and (resid 174 through 304 )C174 - 304
12X-RAY DIFFRACTION12chain 'C' and (resid 305 through 386 )C305 - 386
13X-RAY DIFFRACTION13chain 'C' and (resid 387 through 427 )C387 - 427
14X-RAY DIFFRACTION14chain 'D' and (resid 1 through 173 )D1 - 173
15X-RAY DIFFRACTION15chain 'D' and (resid 174 through 340 )D174 - 340
16X-RAY DIFFRACTION16chain 'D' and (resid 341 through 428 )D341 - 428
17X-RAY DIFFRACTION17chain 'E' and (resid 1 through 46 )E1 - 46
18X-RAY DIFFRACTION18chain 'E' and (resid 47 through 174 )E47 - 174
19X-RAY DIFFRACTION19chain 'E' and (resid 175 through 278 )E175 - 278
20X-RAY DIFFRACTION20chain 'E' and (resid 279 through 304 )E279 - 304
21X-RAY DIFFRACTION21chain 'E' and (resid 305 through 348 )E305 - 348
22X-RAY DIFFRACTION22chain 'E' and (resid 349 through 386 )E349 - 386
23X-RAY DIFFRACTION23chain 'E' and (resid 387 through 428 )E387 - 428
24X-RAY DIFFRACTION24chain 'F' and (resid 1 through 46 )F1 - 46
25X-RAY DIFFRACTION25chain 'F' and (resid 47 through 201 )F47 - 201
26X-RAY DIFFRACTION26chain 'F' and (resid 202 through 302 )F202 - 302
27X-RAY DIFFRACTION27chain 'F' and (resid 303 through 386 )F303 - 386
28X-RAY DIFFRACTION28chain 'F' and (resid 387 through 428 )F387 - 428
29X-RAY DIFFRACTION29chain 'G' and (resid 1 through 46 )G1 - 46
30X-RAY DIFFRACTION30chain 'G' and (resid 47 through 174 )G47 - 174
31X-RAY DIFFRACTION31chain 'G' and (resid 175 through 278 )G175 - 278
32X-RAY DIFFRACTION32chain 'G' and (resid 279 through 348 )G279 - 348
33X-RAY DIFFRACTION33chain 'G' and (resid 349 through 386 )G349 - 386
34X-RAY DIFFRACTION34chain 'G' and (resid 387 through 427 )G387 - 427
35X-RAY DIFFRACTION35chain 'H' and (resid 1 through 46 )H1 - 46
36X-RAY DIFFRACTION36chain 'H' and (resid 47 through 173 )H47 - 173
37X-RAY DIFFRACTION37chain 'H' and (resid 174 through 319 )H174 - 319
38X-RAY DIFFRACTION38chain 'H' and (resid 320 through 386 )H320 - 386
39X-RAY DIFFRACTION39chain 'H' and (resid 387 through 428 )H387 - 428

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